使用电聚合亚甲基蓝和多壁碳纳米管修饰的丝网印刷碳电极对白细胞介素-6 进行比率电化学检测

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-09-25 DOI:10.3390/bios14100457
Zhuo Liu, Fengyu Liu, Chaofan Wang, Hongjuan Li, Yongqian Xu, Shiguo Sun
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引用次数: 0

摘要

在此,我们报告了一种基于比率的电化学生物传感器,用于检测白细胞介素-6(IL-6)。我们在丝网印刷碳电极表面电聚合亚甲基蓝(MB);引入内部参考信号探针;修饰电极表面的羧基多壁碳纳米管以增加电化学活性面积;最后通过希夫碱反应将氨基修饰的 IL-6 合体连接到电极表面,并加入牛血清白蛋白(BSA)以掩蔽非特异性吸附。在样品中加入 IL-6 后,IMB 的信号几乎保持不变,而 I[Fe(CN)6]3-/4- 的信号则随着 IL-6 浓度的增加而降低。因此,一种线性范围为 0.001~1000.0 纳克/毫升、检测限低至 0.54 皮克/毫升的新型比率电化学传感器研制成功。该传感器具有高重复性、稳定性、灵敏度和实用性。它为构建比例电化学传感器和检测 IL-6 提供了一种新方法。
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Ratiometric Electrochemical Detection of Interleukin-6 Using Electropolymerized Methylene Blue and a Multi-Walled Carbon-Nanotube-Modified Screen-Printed Carbon Electrode.

Herein, we report a ratio-based electrochemical biosensor for the detection of interleukin-6 (IL-6). We electropolymerized methylene blue (MB) on the surface of screen-printed carbon electrodes; introduced an internal reference signal probe; modified the carboxylate multi-walled carbon nanotubes on the electrode surface to increase the electrochemically active area; and finally linked the amino-modified IL-6 aptamer to the electrode surface through the Schiff base reaction, with bovine serum albumin (BSA) added to mask non-specific adsorption. After adding IL-6 to the samples, the signal of IMB remained almost unchanged, while the signal of I[Fe(CN)6]3-/4- decreased with increasing IL-6 concentration. Thus, a novel ratiometric electrochemical sensor with a linear range of 0.001~1000.0 ng/mL and a low detection limit of 0.54 pg/mL was successfully developed. The sensor had high repeatability, stability, sensitivity, and practicability. It provides a new method for constructing proportional electrochemical sensors and detecting IL-6.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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