MiR-375:它可能是 1 型糖尿病患者及其兄弟姐妹代谢变化和炎症的一般生物标志物。

IF 5.4 2区 医学 Q1 Medicine Journal of Endocrinological Investigation Pub Date : 2024-10-25 DOI:10.1007/s40618-024-02474-4
Eman A Mostafa, Nagwa Abdallah Ismail, Abeer M Nour El Din Abd El Baky, Tarek F ElShaer, Ingy Ashmawy, Aliaa Ahmed Wahby, Mai Magdy Abdel Wahed, Shereen Hamdy Abd El Aziz
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引用次数: 0

摘要

目的:1 型糖尿病(T1D)是一种慢性自身免疫性疾病,会导致胰岛β细胞丧失和胰岛素分泌不足。微小核糖核酸(miRNAs)与免疫系统功能有关,有助于 T1D 的病理生理学,miRNA-375 在人类胰腺中显著表达,其循环水平可能与β细胞的改变相对应。胰岛细胞抗体(ICA)和谷氨酸脱羧酶抗体(GADA)在自身免疫发病机制中发挥作用,是 T1D 的预测标志物。本研究旨在检测 T1D 患者及其兄弟姐妹与健康对照组相比血清中 miRNA-375、ICA 和 GADA 水平的变化,并将其与 T1D 生化指标相关联:研究对象包括 66 名 T1D 患者(32 名男性和 34 名女性;年龄范围为 3-18 岁)、22 名患者的兄弟姐妹(13 名男性和 9 名女性;年龄范围为 4-17 岁)和 23 名健康对照者(7 名男性和 16 名女性;年龄范围为 4-17 岁)。MiRNA-375 的水平采用定量反转录聚合酶链反应(RT-qPCR)法测定,而 ICA 和 GADA 的水平则采用酶联免疫吸附试验(ELISA)法测定。数据分析采用 SPSS-17 软件:结果:与健康对照组相比,T1D 患者体内的 MiR-375 水平下调,其兄弟姐妹体内的 MiR-375 水平进一步下降。此外,miR-375 与 HbA1c 水平呈反相关,但与胰岛素总剂量、病程或自身抗体(GADA 和 ICA)无相关性:我们的研究表明,miR-375 在 T1D 儿童及其兄弟姐妹中明显下调,这表明它可能成为β细胞功能和血糖控制的生物标志物。
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MiR-375: it could be a general biomarker of metabolic changes and inflammation in type 1 diabetes patients and their siblings.

Purpose: Type 1 diabetes (T1D) is a chronic autoimmune illness that results in loss of pancreatic beta cells and insulin insufficiency. MicroRNAs (miRNAs) are linked to immune system functions contributing to the pathophysiology of T1D, miRNA-375 is significantly expressed in the human pancreas and its circulatory levels might correspond to beta cell alterations. Pancreatic islet cell antibodies (ICA) and Glutamic acid decarboxylase antibodies (GADA) have roles in autoimmune pathogenesis and are predictive markers of T1D. The aim of this work was to detect serum level changes of miRNA-375, ICA, and GADA in T1D patients, and their siblings compared to healthy controls and correlate them with T1D biochemical parameters.

Methods: The study included 66 T1D patients (32 males and 34 females; age range 3-18 years), 22 patients' siblings (13 males and 9 females; age range 4-17 years), and 23 healthy controls (7 males and 16 females; age range 4-17 years). MiRNA-375 levels were measured using quantitative reverse transcription polymerase chain reaction (RT-qPCR), while ICA and GADA levels were measured using enzyme-linked immunosorbent assay (ELISA). Data analysis was done utilizing SPSS-17 software.

Results: MiR-375 levels were downregulated in T1D patients and further decreased in their siblings when compared to healthy controls. Furthermore, miR-375 exhibited inverse correlations with HbA1c levels but no correlations with Total Insulin Dose, disease duration, or autoantibodies (GADA & ICA).

Conclusion: Our study indicates that miR-375 is significantly downregulated in children with T1D and their siblings, suggesting its potential role as a biomarker for beta-cell function and glycemic control.

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来源期刊
Journal of Endocrinological Investigation
Journal of Endocrinological Investigation ENDOCRINOLOGY & METABOLISM-
CiteScore
8.10
自引率
7.40%
发文量
242
期刊介绍: The Journal of Endocrinological Investigation is a well-established, e-only endocrine journal founded 36 years ago in 1978. It is the official journal of the Italian Society of Endocrinology (SIE), established in 1964. Other Italian societies in the endocrinology and metabolism field are affiliated to the journal: Italian Society of Andrology and Sexual Medicine, Italian Society of Obesity, Italian Society of Pediatric Endocrinology and Diabetology, Clinical Endocrinologists’ Association, Thyroid Association, Endocrine Surgical Units Association, Italian Society of Pharmacology.
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