Alina Uribe-García , Estefany I. Medina-Reyes , Carlos A. Flores-Reyes , Alejandro A. Zagal-Salinas , Octavio Ispanixtlahuatl-Meraz , Eduardo Delgado-Armenta , Miguel Santibáñez-Andrade , Cesar M. Flores , Yesennia Sánchez-Pérez , Claudia M. García-Cuéllar , Yolanda I. Chirino
{"title":"食品级二氧化钛诱导结肠细胞内质网应激：正常细胞与结直肠癌细胞的比较","authors":"Alina Uribe-García , Estefany I. Medina-Reyes , Carlos A. Flores-Reyes , Alejandro A. Zagal-Salinas , Octavio Ispanixtlahuatl-Meraz , Eduardo Delgado-Armenta , Miguel Santibáñez-Andrade , Cesar M. Flores , Yesennia Sánchez-Pérez , Claudia M. García-Cuéllar , Yolanda I. Chirino","doi":"10.1016/j.tiv.2024.105957","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Food-grade titanium dioxide (E171) has been under scrutiny in the last decade since its possible adverse effects; however, the cellular mechanisms underlying E171 toxicity have not been thoroughly described.</div></div><div><h3>Aim</h3><div>We aimed to compare the effects of E171 on endoplasmic reticulum (ER) homeostasis in normal and cancer colon cells.</div></div><div><h3>Experimental design</h3><div>We exposed normal, carcinoma, and adenocarcinoma cells to 0.1, 1, 10, 50 and 100 μg/cm<sup>2</sup> of E171 for 24, 48 and 72 h, and we evaluated ER stress, cell viability, titanium uptake, intracellular calcium concentration, and gene expression related to unfolded protein response (UPR) and chaperone pathways.</div></div><div><h3>Results</h3><div>Cell viability decreased only after 72 h of exposure to 100 μg/cm<sup>2</sup> of E171. Adenocarcinoma cells internalized higher titanium amounts than normal and carcinoma cells, but the effects in ER distribution, intracellular calcium concentration, and gene expression were similar among the three cell lines. The expression of UPR and chaperone pathways were downregulated at the lowest concentrations but upregulated at the highest concentrations in the three cell lines.</div></div><div><h3>Conclusion</h3><div>E171 induces ER stress through alterations in ER distribution, intracellular calcium, and UPR and chaperone protein pathways.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"102 ","pages":"Article 105957"},"PeriodicalIF":2.6000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Food grade titanium dioxide induced endoplasmic reticulum stress in colon cells: Comparison between normal and colorectal carcinoma cells\",\"authors\":\"Alina Uribe-García , Estefany I. Medina-Reyes , Carlos A. Flores-Reyes , Alejandro A. Zagal-Salinas , Octavio Ispanixtlahuatl-Meraz , Eduardo Delgado-Armenta , Miguel Santibáñez-Andrade , Cesar M. Flores , Yesennia Sánchez-Pérez , Claudia M. García-Cuéllar , Yolanda I. Chirino\",\"doi\":\"10.1016/j.tiv.2024.105957\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Food-grade titanium dioxide (E171) has been under scrutiny in the last decade since its possible adverse effects; however, the cellular mechanisms underlying E171 toxicity have not been thoroughly described.</div></div><div><h3>Aim</h3><div>We aimed to compare the effects of E171 on endoplasmic reticulum (ER) homeostasis in normal and cancer colon cells.</div></div><div><h3>Experimental design</h3><div>We exposed normal, carcinoma, and adenocarcinoma cells to 0.1, 1, 10, 50 and 100 μg/cm<sup>2</sup> of E171 for 24, 48 and 72 h, and we evaluated ER stress, cell viability, titanium uptake, intracellular calcium concentration, and gene expression related to unfolded protein response (UPR) and chaperone pathways.</div></div><div><h3>Results</h3><div>Cell viability decreased only after 72 h of exposure to 100 μg/cm<sup>2</sup> of E171. Adenocarcinoma cells internalized higher titanium amounts than normal and carcinoma cells, but the effects in ER distribution, intracellular calcium concentration, and gene expression were similar among the three cell lines. The expression of UPR and chaperone pathways were downregulated at the lowest concentrations but upregulated at the highest concentrations in the three cell lines.</div></div><div><h3>Conclusion</h3><div>E171 induces ER stress through alterations in ER distribution, intracellular calcium, and UPR and chaperone protein pathways.</div></div>\",\"PeriodicalId\":54423,\"journal\":{\"name\":\"Toxicology in Vitro\",\"volume\":\"102 \",\"pages\":\"Article 105957\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-10-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxicology in Vitro\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0887233324001875\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicology in Vitro","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0887233324001875","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"TOXICOLOGY","Score":null,"Total":0}
Food grade titanium dioxide induced endoplasmic reticulum stress in colon cells: Comparison between normal and colorectal carcinoma cells
Background
Food-grade titanium dioxide (E171) has been under scrutiny in the last decade since its possible adverse effects; however, the cellular mechanisms underlying E171 toxicity have not been thoroughly described.
Aim
We aimed to compare the effects of E171 on endoplasmic reticulum (ER) homeostasis in normal and cancer colon cells.
Experimental design
We exposed normal, carcinoma, and adenocarcinoma cells to 0.1, 1, 10, 50 and 100 μg/cm2 of E171 for 24, 48 and 72 h, and we evaluated ER stress, cell viability, titanium uptake, intracellular calcium concentration, and gene expression related to unfolded protein response (UPR) and chaperone pathways.
Results
Cell viability decreased only after 72 h of exposure to 100 μg/cm2 of E171. Adenocarcinoma cells internalized higher titanium amounts than normal and carcinoma cells, but the effects in ER distribution, intracellular calcium concentration, and gene expression were similar among the three cell lines. The expression of UPR and chaperone pathways were downregulated at the lowest concentrations but upregulated at the highest concentrations in the three cell lines.
Conclusion
E171 induces ER stress through alterations in ER distribution, intracellular calcium, and UPR and chaperone protein pathways.
期刊介绍：
Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.
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