富精氨酸表面活性剂类肽纳米管与脂质体的相互作用

IF 5.5 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2024-10-29 DOI:10.1021/acs.biomac.4c01072
Valeria Castelletto, Jani Seitsonen, Lucas R de Mello, Ian W Hamley
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引用次数: 0

摘要

研究了在不同 pH 值的水溶液中,带有三个阳离子精氨酸残基的表面活性剂样肽(SLP)R3L12 与模型脂质体之间的相互作用。使用小角 X 射线散射和低温透射电子显微镜探测了阴离子脂质 DPPG [1,2-二棕榈酰-sn-甘油-3-磷-rac-(1-甘油)] 或齐聚糖脂质 DPPE [1,2-二棕榈酰-sn-甘油-3-磷乙醇胺] 模型脂质体的结构。在 R3L12 的存在下,DPPG 的单纤毛膜囊泡发生了明显的重组,尤其是在低 pH 值条件下,DPPE 的多纤毛膜囊泡也在这些条件下发生了重组。通过钙蓝蛋白荧光探测,SLP 可促进囊泡中包裹的货物释放,阴离子 DPPG 囊泡的释放量明显更高。探测膜流动性(脂链有序性)的劳尔登荧光实验表明,R3L12 会破坏脂质凝胶相的稳定性,尤其是阴离子 DPPG。这种纳米管形成的 SLP 模型有望成为囊泡包裹货物的 pH 值敏感释放系统。
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Interaction of Arginine-Rich Surfactant-like Peptide Nanotubes with Liposomes.

The interaction of the surfactant-like peptide (SLP) R3L12 bearing three cationic arginine residues with model liposomes is investigated in aqueous solution at various pH values, under conditions for which the SLP self-assembles into nanotubes. The structure of liposomes of model anionic lipid DPPG [1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol)], or zwitterionic lipid DPPE [1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine] is probed using small-angle X-ray scattering and cryogenic-transmission electron microscopy. The unilamellar vesicles of DPPG are significantly restructured in the presence of R3L12, especially at low pH, and multilamellar vesicles of DPPE are also restructured under these conditions. The SLP promotes the release of cargo encapsulated in the vesicles as probed by calcein fluorescence, with notably higher release for anionic DPPG vesicles. Laurdan fluorescence experiments to probe membrane fluidity (lipid chain ordering) show that R3L12 destabilizes the lipid gel phase, especially for anionic DPPG. This model nanotube-forming SLP has promise as a pH-sensitive release system for vesicle-encapsulated cargo.

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来源期刊
Biomacromolecules
Biomacromolecules 化学-高分子科学
CiteScore
10.60
自引率
4.80%
发文量
417
审稿时长
1.6 months
期刊介绍: Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.
期刊最新文献
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