拟南芥 DREB26/ERF12 及其近缘种调控干旱胁迫条件下的角蜡生物合成。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-28 DOI:10.1111/tpj.17100
Kaoru Urano, Yoshimi Oshima, Toshiki Ishikawa, Takuma Kajino, Shingo Sakamoto, Mayuko Sato, Kiminori Toyooka, Miki Fujita, Maki Kawai-Yamada, Teruaki Taji, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki
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引用次数: 0

摘要

陆生植物为了适应陆地环境,在气生器官表面进化出疏水的角质层,以确保在陆地环境中生存。角质层主要由脂类组成,即角质素和角质内蜡,表皮蜡沉积在植物表面。角质层的组成和渗透性对植物抵御干旱胁迫的能力有很大影响。然而,干旱胁迫下角质层蜡生物合成的调控机制尚未完全阐明。在这里,我们发现了三个参与调控植物表面透水性的 AP2/ERF 转录因子(DREB26/ERF12、ERF13 和 ERF14)。透射电子显微镜显示,过表达 DREB26(DREB26OX)的植株叶片角质层较厚,而表达 SRDX 抑制结构域融合 DREB26(DREB26SR)的转基因植株叶片角质层较薄。参与角质层蜡形成的基因在 DREB26OX 中上调,而在 DREB26SR 中下调。作为蜡的主要成分的超长链(VLC)烷烃的含量在 DREB26OX 叶片中增加,而在 DREB26SR 叶片中减少。在脱水胁迫下,DREB26OX 的水分损失减少,而 DREB26SR 的水分损失增加。erf12/13/14三重突变体表现出生长延迟、叶片含水量降低以及干旱诱导的VLC烷烃积累减少。综上所述,我们的研究结果表明,DREB26/ERF12及其闭合家族成员ERF13和ERF14在干旱胁迫下的角质蜡生物合成中发挥着重要作用。本文讨论了干旱胁迫条件下参与调控角蜡生物合成的复杂转录级联。
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Arabidopsis DREB26/ERF12 and its close relatives regulate cuticular wax biosynthesis under drought stress condition.

Land plants have evolved a hydrophobic cuticle on the surface of aerial organs as an adaptation to ensure survival in terrestrial environments. Cuticle is mainly composed of lipids, namely cutin and intracuticular wax, with epicuticular wax deposited on plant surface. The composition and permeability of cuticle have a large influence on its ability to protect plants against drought stress. However, the regulatory mechanisms underlying cuticular wax biosynthesis in response to drought stress have not been fully elucidated. Here, we identified three AP2/ERF transcription factors (DREB26/ERF12, ERF13 and ERF14) involved in the regulation of water permeability of the plant surface. Transmission electron microscopy revealed thicker cuticle on the leaves of DREB26-overexpressing (DREB26OX) plants, and thinner cuticle on the leaves of transgenic plants expressing SRDX repression domain-fused DREB26 (DREB26SR). Genes involved in cuticular wax formation were upregulated in DREB26OX and downregulated in DREB26SR. The levels of very-long chain (VLC) alkanes, which are a major wax component, increased in DREB26OX leaves and decreased in DREB26SR leaves. Under dehydration stress, water loss was reduced in DREB26OX and increased in DREB26SR. The erf12/13/14 triple mutant showed delayed growth, decreased leaf water content, and reduced drought-inducible VLC alkane accumulation. Taken together, our results indicate that the DREB26/ERF12 and its closed family members, ERF13 and ERF14, play an important role in cuticular wax biosynthesis in response to drought stress. The complex transcriptional cascade involved in the regulation of cuticular wax biosynthesis under drought stress conditions is discussed.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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