NaF 和 NaBr 诱导的 DNA/金纳米粒子共轭体系具有优异的性能:稳定性更好、修饰时间更短、负载能力更强。

IF 10.7 1区 生物学 Q1 BIOPHYSICS Biosensors and Bioelectronics Pub Date : 2024-10-24 DOI:10.1016/j.bios.2024.116876
Hai-Bo Wang, Liang Zhang, Tian-Yu Hu, Xue-Qing Yuan, Sheng-Wei Huang, Jin-Quan Li, Zi-Tao Zhong, Yuan-Di Zhao
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引用次数: 0

摘要

金纳米粒子(AuNP)的功能化是生化和生物医学应用的关键步骤。传统的盐老化方法需要逐步加入 NaCl 和过量的硫醇化 DNA,这主要是由于 DNA/AuNP 混合物对 NaCl 的耐受性较差。在这里,我们发现 NaF 能够提高不同碱基 DNA 序列(poly A/T/C/G)修饰 AuNP 的稳定性,并允许一次性加入 200 mM 的高浓度,从而将总修饰时间大大缩短至 0.5-1 h。除了 NaF 和 NaBr 的优点外,针对富含 G 的 DNA 序列,通过引入寡 A/T 间距,改性效果也得到了提高。此外,该方法在装载能力或序列成分方面均优于另外两种方法(基于 pH 3 的方法和盐老化方法)。
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Excellent properties of NaF and NaBr induced DNA/gold nanoparticle conjugation system: Better stability, shorter modified time, and higher loading capacity.

The functionalization of gold nanoparticle (AuNP) is the key procedure for the biochemical and biomedical application. The conventional salt-aging method requires the stepwise additions of NaCl and excessive thiolated DNA, mainly due to the poor tolerance of the DNA/AuNP mixture toward NaCl. Herein, we found that NaF is capable of improving the stability for the modification of AuNP with different bases of DNA sequences (poly A/T/C/G), and allows for adding up with a high concentration of 200 mM at one time, which greatly reduces the total modification time to 0.5-1 h. Intriguingly, the introduction of NaBr effectively increases the DNA loading capacity. Besides the advantages of NaF and NaBr, the modification performance is improved via the introduction of the oligo A/T spacer for the G-rich DNA sequences. Furthermore, this method shows the superiority to another two methods (pH 3-based and salt-aging) in terms of the loading capacity or sequence components.

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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
期刊最新文献
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