八子布神通过调节 PI3K-AKT 和细胞凋亡途径减轻 SAMP6 小鼠的骨质疏松症。

Zhe Xu, Zeyu Zhang, Huifang Zhou, Shan Lin, Boyang Gong, Zhaodong Li, Shuwu Zhao, Yunlong Hou, Yanfei Peng, Yuhong Bian
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引用次数: 0

摘要

骨质疏松症(Osteoporosis,OP)是一种全身性骨骼疾病,以低骨量、骨组织退化和骨微结构紊乱为特征。中成药八味布参已被证实能有效减轻骨质疏松症,但其药理机制仍不清楚。本研究采用衰老加速小鼠易感 6(SAMP6)模型探讨骨稳态,并用巴子布参进行胃内治疗 9 周。体内实验表明,八子布神不仅能提高骨矿密度和骨矿含量,还能增加 RUNX2 和 OSX 的含量。此外,通过对 SAMP6 小鼠骨间充质干细胞(BMSCs)的原代培养,验证了巴子布神对成骨细胞和脂肪细胞分化平衡以及 ROS 和衰老水平的影响。最后,我们通过网络药理学和实验验证研究了巴子布神减轻OP的药理机制,发现巴子布神可以通过刺激PI3K-Akt和抑制细胞凋亡来显著协调骨稳态和减轻OP的进展。综上所述,我们的研究首次揭示了八味布神通过调控PI3K-AKT和细胞凋亡通路来协调骨稳态,从而减轻OP病情的证据。
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Bazi Bushen attenuates osteoporosis in SAMP6 mice by regulating PI3K-AKT and apoptosis pathways

Osteoporosis (OP), a systemic skeletal disease, is characterized by low bone mass, bone tissue degradation and bone microarchitecture disturbance. Bazi Bushen, a Chinese patented medicine, has been demonstrated to be effective in attenuating OP, but the pharmacological mechanism remains predominantly unclear. In this study, the senescence-accelerated mouse prone 6 (SAMP6) model was used to explore bone homeostasis and treated intragastrically for 9 weeks with Bazi Bushen. In vivo experiments showed that Bazi Bushen treatment not only upregulated the levels of bone mineral density and bone mineral content but also increased the content of RUNX2 and OSX. Furthermore, the primary culture of bone mesenchymal stem cells (BMSCs) in SAMP6 mice was used to verify the effects of Bazi Bushen on the balance of differentiation between osteoblasts and adipocytes, as well as ROS and aging levels. Finally, the pharmacological mechanism of Bazi Bushen in attenuating OP was investigated through network pharmacology and experimental verification, and we found that Bazi Bushen could significantly orchestrate bone homeostasis and attenuate the progression of OP by stimulating PI3K-Akt and inhibiting apoptosis. In summary, our work sheds light on the first evidence that Bazi Bushen attenuates OP by regulating PI3K-AKT and apoptosis pathways to orchestrate bone homeostasis.

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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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