Taynara Simão Matos, Flávia da Silva Zandonadi, Alex Aparecido Rosini Silva, Stephanie Dias Soares, Adriana de Souza Lima, Glaucia Maria Pastore, Andreia de Melo Porcari, Alessandra Sussulini
{"title":"利用代谢组学根据制备方式和植物品种区分青藤样本","authors":"Taynara Simão Matos, Flávia da Silva Zandonadi, Alex Aparecido Rosini Silva, Stephanie Dias Soares, Adriana de Souza Lima, Glaucia Maria Pastore, Andreia de Melo Porcari, Alessandra Sussulini","doi":"10.1080/02791072.2024.2420059","DOIUrl":null,"url":null,"abstract":"<p><p>Ayahuasca is a brew traditionally prepared with a mixture of <i>Psychotria viridis</i> leaves and <i>Banisteriopsis caapi</i> vine and has demonstrated therapeutic properties for depression. Knowledge of the brew composition is important to improve the therapeutic potential and decrease side effects if ayahuasca becomes an option for refractory depression treatment. Ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) was applied to analyze 126 ayahuasca samples collected from different <i>ayahuasqueiro</i> groups and geographic origins. We were able to observe a differentiation in the metabolite composition of ayahuasca samples prepared by diverse <i>ayahuasqueiro</i> groups. These samples presented different antioxidant effects based on FRAP and ORAC assays. Exploratory statistical analysis demonstrated a trend of separating the samples according to the religious group. The most important identified compounds for differentiation of the brew prepared by distinct religious groups are glycosylated and/or phenolic compounds. The comparison based on the mode of ayahuasca preparation presented more variability than the comparison based on the botanical variety of <i>B. caapi</i> used. We conclude that ayahuasca samples prepared with \"caupuri\" or \"tucunacá\" separately exhibited differences in the analysis of L-glutamate and the metabolism of arginine and proline. This suggests that a possible variation in this pathway could explain the occurrence of swollen stem nodes in \"caupuri,\" one of the <i>B. caapi</i> varieties.</p>","PeriodicalId":16902,"journal":{"name":"Journal of psychoactive drugs","volume":" ","pages":"1-10"},"PeriodicalIF":2.1000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differentiation of Ayahuasca Samples According to Preparation Mode and Botanical Varieties Using Metabolomics.\",\"authors\":\"Taynara Simão Matos, Flávia da Silva Zandonadi, Alex Aparecido Rosini Silva, Stephanie Dias Soares, Adriana de Souza Lima, Glaucia Maria Pastore, Andreia de Melo Porcari, Alessandra Sussulini\",\"doi\":\"10.1080/02791072.2024.2420059\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Ayahuasca is a brew traditionally prepared with a mixture of <i>Psychotria viridis</i> leaves and <i>Banisteriopsis caapi</i> vine and has demonstrated therapeutic properties for depression. Knowledge of the brew composition is important to improve the therapeutic potential and decrease side effects if ayahuasca becomes an option for refractory depression treatment. Ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) was applied to analyze 126 ayahuasca samples collected from different <i>ayahuasqueiro</i> groups and geographic origins. We were able to observe a differentiation in the metabolite composition of ayahuasca samples prepared by diverse <i>ayahuasqueiro</i> groups. These samples presented different antioxidant effects based on FRAP and ORAC assays. Exploratory statistical analysis demonstrated a trend of separating the samples according to the religious group. The most important identified compounds for differentiation of the brew prepared by distinct religious groups are glycosylated and/or phenolic compounds. The comparison based on the mode of ayahuasca preparation presented more variability than the comparison based on the botanical variety of <i>B. caapi</i> used. We conclude that ayahuasca samples prepared with \\\"caupuri\\\" or \\\"tucunacá\\\" separately exhibited differences in the analysis of L-glutamate and the metabolism of arginine and proline. This suggests that a possible variation in this pathway could explain the occurrence of swollen stem nodes in \\\"caupuri,\\\" one of the <i>B. caapi</i> varieties.</p>\",\"PeriodicalId\":16902,\"journal\":{\"name\":\"Journal of psychoactive drugs\",\"volume\":\" \",\"pages\":\"1-10\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-10-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of psychoactive drugs\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/02791072.2024.2420059\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PSYCHOLOGY, CLINICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of psychoactive drugs","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02791072.2024.2420059","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PSYCHOLOGY, CLINICAL","Score":null,"Total":0}
Differentiation of Ayahuasca Samples According to Preparation Mode and Botanical Varieties Using Metabolomics.
Ayahuasca is a brew traditionally prepared with a mixture of Psychotria viridis leaves and Banisteriopsis caapi vine and has demonstrated therapeutic properties for depression. Knowledge of the brew composition is important to improve the therapeutic potential and decrease side effects if ayahuasca becomes an option for refractory depression treatment. Ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) was applied to analyze 126 ayahuasca samples collected from different ayahuasqueiro groups and geographic origins. We were able to observe a differentiation in the metabolite composition of ayahuasca samples prepared by diverse ayahuasqueiro groups. These samples presented different antioxidant effects based on FRAP and ORAC assays. Exploratory statistical analysis demonstrated a trend of separating the samples according to the religious group. The most important identified compounds for differentiation of the brew prepared by distinct religious groups are glycosylated and/or phenolic compounds. The comparison based on the mode of ayahuasca preparation presented more variability than the comparison based on the botanical variety of B. caapi used. We conclude that ayahuasca samples prepared with "caupuri" or "tucunacá" separately exhibited differences in the analysis of L-glutamate and the metabolism of arginine and proline. This suggests that a possible variation in this pathway could explain the occurrence of swollen stem nodes in "caupuri," one of the B. caapi varieties.