Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Maryam Taghipour-Shahbandi, Amir Reza Sabzian, Ali Reza Yousefi, Mahboubeh Khajehnabi, Woo Kyun Kim
{"title":"探究日本鹌鹑（Coturnix japonica）整个生育期与营养吸收相关的基因和肠道组织学。","authors":"Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Maryam Taghipour-Shahbandi, Amir Reza Sabzian, Ali Reza Yousefi, Mahboubeh Khajehnabi, Woo Kyun Kim","doi":"10.1002/vms3.70056","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.</p><p><strong>Objectives: </strong>This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.</p><p><strong>Methods: </strong>A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.</p><p><strong>Results: </strong>Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).</p><p><strong>Conclusions: </strong>In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519991/pdf/","citationCount":"0","resultStr":"{\"title\":\"Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan.\",\"authors\":\"Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Maryam Taghipour-Shahbandi, Amir Reza Sabzian, Ali Reza Yousefi, Mahboubeh Khajehnabi, Woo Kyun Kim\",\"doi\":\"10.1002/vms3.70056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.</p><p><strong>Objectives: </strong>This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.</p><p><strong>Methods: </strong>A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.</p><p><strong>Results: </strong>Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).</p><p><strong>Conclusions: </strong>In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.</p>\",\"PeriodicalId\":23543,\"journal\":{\"name\":\"Veterinary Medicine and Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519991/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Medicine and Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1002/vms3.70056\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.70056","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan.
Background: Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.
Objectives: This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.
Methods: A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.
Results: Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).
Conclusions: In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.
期刊介绍：
Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science.
Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.
We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell.
Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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