伊朗西北部省份牲畜中法氏囊病菌的形态和分子特征。

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Veterinary Medicine and Science Pub Date : 2024-11-01 DOI:10.1002/vms3.70097
Hossein Galavani, Saber Raeghi, Mehdi Karamian, Negin Torabi, Ali Haniloo
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引用次数: 0

摘要

背景:法氏囊属肝吸虫是人类和牲畜法氏囊病的病原体。准确鉴定法氏囊属对了解法氏囊病的流行病学至关重要。本研究旨在确定伊朗西北部省份牲畜中法氏囊虫的形态和分子特征:方法:2021 年 9 月至 2022 年 8 月期间,在伊朗西北部省份(西阿塞拜疆、东阿塞拜疆、阿尔达比勒和赞詹)的四个当地屠宰场,从不同的确定宿主(牛、绵羊、山羊和水牛)身上采集了 500 只成年法氏囊虫。所有样本均通过形态测定标准进行了检验;然后,利用基于 ITS1 区域的 PCR-RFLP 方法对 49 个样本进行了鉴定,并利用 cox1 标记对 23 个分离物序列进行了分析。PCR-RFLP方法比较了形态测量结果,cox1基因序列用于确认PCR-RFLP结果和系统进化分析:结果:两个物种的体长、体宽、头锥长、头锥宽、体面积、腹吸盘与身体后端的距离差异显著(P < 0.05)。根据形态计量标准,139 个样本(27.8%)被鉴定为巨型法氏囊虫,361 个样本(72.2%)被鉴定为肝脏法氏囊虫。同样,ITS1 区域的 PCR-RFLP 分析也证实了形态测定的结果。没有检测到杂交型法氏囊。cox1 的部分序列显示,肝包虫有 13 个可变位点,其中有 8 个单倍型,而巨大包虫有 12 个可变位点,其中有 5 个单倍型:结论:根据本研究的结果,PCR-RFLP 方法可用于确认 Fasciola 物种的形态学方法,但不足以研究其遗传多样性。此外,本研究的 cox1 序列结果表明,伊朗北部省份的 F. hepatica 和 F. gigantica 具有不同的遗传结构和单倍型。
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Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran.

Background: Liver flukes from the genus Fasciola are the causative agents for human and livestock fascioliasis. Accurate identification of Fasciola spp. is essential to understanding the epidemiology of fascioliasis. This study aimed to determine the morphometric and molecular characterization of Fasciola spp. in livestock from Northwestern provinces of Iran.

Methods: Five hundred adult Fasciola flukes were obtained from different definitive hosts (cattle, sheep, goats, and buffaloes) in four local abattoirs in the northwestern provinces of Iran (West-Azerbaijan, East-Azerbaijan, Ardabil, and Zanjan) from September 2021 to August 2022. All samples were examined by morphometric criteria; then, 49 samples were identified using PCR-RFLP based on ITS1 region and 23 sequence of isolates analyzed by cox1 marker. PCR-RFLP methods compared morphometric results, and cox1 gene sequences were used to confirm PCR-RFLP results and phylogenetic analysis.

Results: The differences between the body length, body width, cephalic cone length, cephalic cone width, body area, and distance between the ventral sucker and posterior end of the body in two species were significant (p < 0.05). Based on the morphometric criteria, 139 samples (27.8%) were identified as Fasciola gigantica and 361 (72.2%) as Fasciola hepatica. Similarly, PCR-RFLP analysis of ITS1 region confirmed morphometric results. No hybrid forms of Fasciola were detected. Partial sequences of cox1 showed 13 variable sites with eight haplotypes in F. hepatica and 12 variable sites with five haplotypes in F. gigantica.

Conclusion: Based on the results of this study, the PCR-RFLP method can be used to confirm the morphological method of Fasciola species, but it is insufficient to study their genetic diversity. Also, sequences of cox1 results of the present study showed that F. hepatica and F. gigantica species in the Northern provinces of Iran have different genetic structures and haplotypes.

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来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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