Xueping Zou, Daniel C. Spencer, Junyu Chen and Hywel Morgan
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引用次数: 0
摘要
我们开发了一种变形性细胞仪,可同时测量粘弹性剪切流中单细胞的光学和电学形状变化。单细胞的光学变形性是通过一个低成本的 CMOS 摄像头测量的,该摄像头由高功率 LED 照亮,并由通过的细胞产生的电阻抗信号触发。同时,使用电极阵列测量沿不同轴线的形状变化,从而确定细胞的电变形性。这是通过将捕捉到的光信号和电信号相关联而实现的,无需复杂的同步。通过测量经细胞松弛素 D、latrunculin B 和戊二醛处理的 HL-60 细胞的变形性,对该系统进行了表征。结果表明,光学和电学方法之间具有极佳的相关性。
A combined electro-optical deformability micro-cytometer†
We have developed a deformability cytometer that simultaneously measures the optical and electrical shape change of single cells in a viscoelastic shear flow. The optical deformability of single cells is measured using a low-cost CMOS camera illuminated with a high-power LED triggered from an electrical impedance signal created by a passing cell. Simultaneously the electrical deformability of the cell is determined using electrode arrays that measure shape changes along different axes. This is achieved by correlating the optical and electrical signals captured without complicated synchronisation. The system was characterised by measuring the deformability of HL-60 cells treated with cytochalasin D, latrunculin B and glutaraldehyde. Results demonstrate excellent correlation between the optical and electrical methods.
期刊介绍:
An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.