转录因子 TaNF-YB4 在小麦中的过表达可提高植物活力和产量

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2024-10-10 DOI:10.1016/j.cpb.2024.100394
{"title":"转录因子 TaNF-YB4 在小麦中的过表达可提高植物活力和产量","authors":"","doi":"10.1016/j.cpb.2024.100394","DOIUrl":null,"url":null,"abstract":"<div><div>Addressing food security is a priority in developing countries. This study aimed to improve wheat yield by overexpressing the <em>TaNF-YB4</em> transcription factor, which is involved in carbon assimilation and stress tolerance. An expression cassette for <em>TaNF-YB4</em> was developed in a modified wheat transformation vector (pSB219) and examined through transient expression in <em>Nicotiana tabacum</em>, followed by <em>Agrobacterium</em>-mediated transformation of wheat variety FSD-2008. T<sub>0</sub> transgenic plants were propagated to obtain T<sub>3</sub> generation PCR-positive plants. Transgene expression was assessed in PCR-verified T<sub>2</sub> plants using RT-PCR and qRT-PCR at six weeks post-germination. qRT-PCR analysis using the ΔΔCT method indicated higher <em>TaNF-YB4</em> expression in transgenic lines than in the wild-type control plants. Improved agronomic and phenotypic traits were observed with a 6–36 % increase in 1000-grain weight in the selected transgenic lines. Root architecture assessments demonstrated enhanced root length, surface area, and projected area in transgenic lines compared with wild-type plants. Additionally, notable variances in total chlorophyll, protein, and sugar content levels were observed between the transgenic lines and control plants, demonstrating statistical significance with a p-value ≤ 0.05. This study indicates that low-level constitutive expression of <em>TaNF-YB4</em> can enhance wheat yield, presenting a viable strategy for improving wheat productivity.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The transcription factor TaNF-YB4 overexpression in wheat increases plant vigor and yield\",\"authors\":\"\",\"doi\":\"10.1016/j.cpb.2024.100394\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Addressing food security is a priority in developing countries. This study aimed to improve wheat yield by overexpressing the <em>TaNF-YB4</em> transcription factor, which is involved in carbon assimilation and stress tolerance. An expression cassette for <em>TaNF-YB4</em> was developed in a modified wheat transformation vector (pSB219) and examined through transient expression in <em>Nicotiana tabacum</em>, followed by <em>Agrobacterium</em>-mediated transformation of wheat variety FSD-2008. T<sub>0</sub> transgenic plants were propagated to obtain T<sub>3</sub> generation PCR-positive plants. Transgene expression was assessed in PCR-verified T<sub>2</sub> plants using RT-PCR and qRT-PCR at six weeks post-germination. qRT-PCR analysis using the ΔΔCT method indicated higher <em>TaNF-YB4</em> expression in transgenic lines than in the wild-type control plants. Improved agronomic and phenotypic traits were observed with a 6–36 % increase in 1000-grain weight in the selected transgenic lines. Root architecture assessments demonstrated enhanced root length, surface area, and projected area in transgenic lines compared with wild-type plants. Additionally, notable variances in total chlorophyll, protein, and sugar content levels were observed between the transgenic lines and control plants, demonstrating statistical significance with a p-value ≤ 0.05. This study indicates that low-level constitutive expression of <em>TaNF-YB4</em> can enhance wheat yield, presenting a viable strategy for improving wheat productivity.</div></div>\",\"PeriodicalId\":38090,\"journal\":{\"name\":\"Current Plant Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Plant Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000768\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000768","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

解决粮食安全问题是发展中国家的当务之急。本研究旨在通过过表达参与碳同化和抗逆的转录因子 TaNF-YB4 来提高小麦产量。在改良的小麦转化载体(pSB219)中开发了 TaNF-YB4 的表达盒,并通过在烟草中的瞬时表达进行了检验,然后用农杆菌介导转化了小麦品种 FSD-2008。T0 转基因植株通过繁殖获得 T3 代 PCR 阳性植株。利用 ΔΔCT 方法进行的 qRT-PCR 分析表明,转基因品系中 TaNF-YB4 的表达高于野生型对照植株。所选转基因品系的农艺性状和表型特征得到了改善,千粒重增加了 6-36%。根系结构评估表明,与野生型植物相比,转基因品系的根系长度、表面积和投影面积都有所增加。此外,还观察到转基因品系与对照植株在总叶绿素、蛋白质和糖含量水平上存在显著差异,p 值≤ 0.05,具有统计学意义。这项研究表明,TaNF-YB4 的低水平组成型表达可提高小麦产量,为提高小麦产量提供了一种可行的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The transcription factor TaNF-YB4 overexpression in wheat increases plant vigor and yield
Addressing food security is a priority in developing countries. This study aimed to improve wheat yield by overexpressing the TaNF-YB4 transcription factor, which is involved in carbon assimilation and stress tolerance. An expression cassette for TaNF-YB4 was developed in a modified wheat transformation vector (pSB219) and examined through transient expression in Nicotiana tabacum, followed by Agrobacterium-mediated transformation of wheat variety FSD-2008. T0 transgenic plants were propagated to obtain T3 generation PCR-positive plants. Transgene expression was assessed in PCR-verified T2 plants using RT-PCR and qRT-PCR at six weeks post-germination. qRT-PCR analysis using the ΔΔCT method indicated higher TaNF-YB4 expression in transgenic lines than in the wild-type control plants. Improved agronomic and phenotypic traits were observed with a 6–36 % increase in 1000-grain weight in the selected transgenic lines. Root architecture assessments demonstrated enhanced root length, surface area, and projected area in transgenic lines compared with wild-type plants. Additionally, notable variances in total chlorophyll, protein, and sugar content levels were observed between the transgenic lines and control plants, demonstrating statistical significance with a p-value ≤ 0.05. This study indicates that low-level constitutive expression of TaNF-YB4 can enhance wheat yield, presenting a viable strategy for improving wheat productivity.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
期刊最新文献
Effect of biostimulants on the chemical profile of food crops under normal and abiotic stress conditions Sustainable nitrogen solutions: Cyanobacteria-powered plant biotechnology for conservation and metabolite production Metabolomic analyses during chayote (Sechium edule var. virens levis) seed germination under the influence of growth regulators Arabidopsis B-BOX DOMAIN PROTEIN14/15/16 form a feedback loop with ELONGATED HYPOCOTYL 5 and PHYTOCHROME-INTERACTING FACTORs to regulate hypocotyl elongation Genome-wide identification of TCP transcription factors and functional role of UrTCP4 in regulating terpenoid indole alkaloids biosynthesis in Uncaria rhynchophylla
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1