马尾藻角叉菜褐藻糖低聚糖:针对 MAPK 和 KEAP1-NRF2 信号通路的纯化、表征和抗氧化作用

IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2024-10-19 DOI:10.1016/j.algal.2024.103756
Shengyu Fan , Ying Xu , Lei Wang , Xin Gao , Soon-Mi Shim , Xiaoting Fu
{"title":"马尾藻角叉菜褐藻糖低聚糖:针对 MAPK 和 KEAP1-NRF2 信号通路的纯化、表征和抗氧化作用","authors":"Shengyu Fan ,&nbsp;Ying Xu ,&nbsp;Lei Wang ,&nbsp;Xin Gao ,&nbsp;Soon-Mi Shim ,&nbsp;Xiaoting Fu","doi":"10.1016/j.algal.2024.103756","DOIUrl":null,"url":null,"abstract":"<div><div>The current research looked into the inhibitory effect of a purified component of <em>Sargassum hornery</em> fucoidan oligosaccharide (F2) with a sulfate content of 16.35 %, which showed substantial protective effects on H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in RAW 264.7 cells and zebrafish. The chemical and structural characteristics were examined by GC, FTIR, MS, and NMR spectroscopy, revealing that F2 was composed of not only a mixed oligosaccharide with 1–4 degrees of polymerization but also had a main chain consisting of (1 → 3) linked α-L-Fuc<em>p</em> and (1 → 3,4) linked α-L-Fuc<em>p</em>, sulfated groups mainly at C2 position. It was found that F2 could activate the MAPK and Keap1-Nrf2 signaling pathways through the dissociation of the Keap1-Nrf2 complex in RAW 264.7 cells. Then Nrf2 entered the cell and bound to the antioxidant response element (ARE), resulting in activating the expression of downstream antioxidant genes. F2 was also found to have a strong protective effect on oxidative stress in zebrafish induced by H<sub>2</sub>O<sub>2</sub>, which can reduce the heart rate of zebrafish and prevent the generation of intracellular ROS and cell death. Therefore, <em>S. hornery</em> fucoidan oligosaccharide (F2) could be a potential ingredient for functional foods, cosmetics, and pharmaceuticals because of its antioxidant activity.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103756"},"PeriodicalIF":4.6000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sargassum hornery fucoidan oligosaccharide: Purification, characterization, and antioxidant effects targeting the MAPK and KEAP1-NRF2 signaling pathways\",\"authors\":\"Shengyu Fan ,&nbsp;Ying Xu ,&nbsp;Lei Wang ,&nbsp;Xin Gao ,&nbsp;Soon-Mi Shim ,&nbsp;Xiaoting Fu\",\"doi\":\"10.1016/j.algal.2024.103756\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The current research looked into the inhibitory effect of a purified component of <em>Sargassum hornery</em> fucoidan oligosaccharide (F2) with a sulfate content of 16.35 %, which showed substantial protective effects on H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in RAW 264.7 cells and zebrafish. The chemical and structural characteristics were examined by GC, FTIR, MS, and NMR spectroscopy, revealing that F2 was composed of not only a mixed oligosaccharide with 1–4 degrees of polymerization but also had a main chain consisting of (1 → 3) linked α-L-Fuc<em>p</em> and (1 → 3,4) linked α-L-Fuc<em>p</em>, sulfated groups mainly at C2 position. It was found that F2 could activate the MAPK and Keap1-Nrf2 signaling pathways through the dissociation of the Keap1-Nrf2 complex in RAW 264.7 cells. Then Nrf2 entered the cell and bound to the antioxidant response element (ARE), resulting in activating the expression of downstream antioxidant genes. F2 was also found to have a strong protective effect on oxidative stress in zebrafish induced by H<sub>2</sub>O<sub>2</sub>, which can reduce the heart rate of zebrafish and prevent the generation of intracellular ROS and cell death. Therefore, <em>S. hornery</em> fucoidan oligosaccharide (F2) could be a potential ingredient for functional foods, cosmetics, and pharmaceuticals because of its antioxidant activity.</div></div>\",\"PeriodicalId\":7855,\"journal\":{\"name\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"volume\":\"84 \",\"pages\":\"Article 103756\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-10-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2211926424003680\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2211926424003680","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目前的研究探讨了马尾藻角叉菜褐藻糖低聚糖(F2)纯化成分的抑制作用,其硫酸盐含量为 16.35%,对 H2O2 诱导的 RAW 264.7 细胞和斑马鱼氧化损伤具有显著的保护作用。通过气相色谱、傅立叶变换红外光谱、质谱和核磁共振光谱研究了其化学和结构特征,发现 F2 不仅由 1-4 度聚合的混合寡糖组成,而且其主链由 (1 → 3) 连接的 α-L-Fucp 和 (1 → 3,4) 连接的 α-L-Fucp 组成,硫酸基团主要位于 C2 位。研究发现,F2 可通过解离 RAW 264.7 细胞中的 Keap1-Nrf2 复合物激活 MAPK 和 Keap1-Nrf2 信号通路。然后,Nrf2进入细胞并与抗氧化反应元件(ARE)结合,从而激活下游抗氧化基因的表达。研究还发现,F2 对 H2O2 诱导的斑马鱼氧化应激有很强的保护作用,能降低斑马鱼的心率,防止细胞内 ROS 的产生和细胞死亡。因此,S. hornery褐藻糖胶低聚糖(F2)因其抗氧化活性可成为功能性食品、化妆品和药品的潜在成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Sargassum hornery fucoidan oligosaccharide: Purification, characterization, and antioxidant effects targeting the MAPK and KEAP1-NRF2 signaling pathways
The current research looked into the inhibitory effect of a purified component of Sargassum hornery fucoidan oligosaccharide (F2) with a sulfate content of 16.35 %, which showed substantial protective effects on H2O2-induced oxidative damage in RAW 264.7 cells and zebrafish. The chemical and structural characteristics were examined by GC, FTIR, MS, and NMR spectroscopy, revealing that F2 was composed of not only a mixed oligosaccharide with 1–4 degrees of polymerization but also had a main chain consisting of (1 → 3) linked α-L-Fucp and (1 → 3,4) linked α-L-Fucp, sulfated groups mainly at C2 position. It was found that F2 could activate the MAPK and Keap1-Nrf2 signaling pathways through the dissociation of the Keap1-Nrf2 complex in RAW 264.7 cells. Then Nrf2 entered the cell and bound to the antioxidant response element (ARE), resulting in activating the expression of downstream antioxidant genes. F2 was also found to have a strong protective effect on oxidative stress in zebrafish induced by H2O2, which can reduce the heart rate of zebrafish and prevent the generation of intracellular ROS and cell death. Therefore, S. hornery fucoidan oligosaccharide (F2) could be a potential ingredient for functional foods, cosmetics, and pharmaceuticals because of its antioxidant activity.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Algal Research-Biomass Biofuels and Bioproducts
Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
9.40
自引率
7.80%
发文量
332
期刊介绍: Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment
期刊最新文献
Deep sequencing analysis of chloroplast transcription and splicing in Euglena gracilis Genetic transformation of the freshwater diatom Cyclotella meneghiniana via bacterial conjugation Comparison of CRISPR/Cas9 and Cas12a for gene editing in Chlamydomonas reinhardtii Effect of temperature on the oxygen production capacity and growth of scenedesmus almeriensis A multi-dimensional comparative study on the performance of algae removal using various flotation
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1