{"title":"马尾藻角叉菜褐藻糖低聚糖:针对 MAPK 和 KEAP1-NRF2 信号通路的纯化、表征和抗氧化作用","authors":"","doi":"10.1016/j.algal.2024.103756","DOIUrl":null,"url":null,"abstract":"<div><div>The current research looked into the inhibitory effect of a purified component of <em>Sargassum hornery</em> fucoidan oligosaccharide (F2) with a sulfate content of 16.35 %, which showed substantial protective effects on H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in RAW 264.7 cells and zebrafish. The chemical and structural characteristics were examined by GC, FTIR, MS, and NMR spectroscopy, revealing that F2 was composed of not only a mixed oligosaccharide with 1–4 degrees of polymerization but also had a main chain consisting of (1 → 3) linked α-L-Fuc<em>p</em> and (1 → 3,4) linked α-L-Fuc<em>p</em>, sulfated groups mainly at C2 position. It was found that F2 could activate the MAPK and Keap1-Nrf2 signaling pathways through the dissociation of the Keap1-Nrf2 complex in RAW 264.7 cells. Then Nrf2 entered the cell and bound to the antioxidant response element (ARE), resulting in activating the expression of downstream antioxidant genes. F2 was also found to have a strong protective effect on oxidative stress in zebrafish induced by H<sub>2</sub>O<sub>2</sub>, which can reduce the heart rate of zebrafish and prevent the generation of intracellular ROS and cell death. Therefore, <em>S. hornery</em> fucoidan oligosaccharide (F2) could be a potential ingredient for functional foods, cosmetics, and pharmaceuticals because of its antioxidant activity.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sargassum hornery fucoidan oligosaccharide: Purification, characterization, and antioxidant effects targeting the MAPK and KEAP1-NRF2 signaling pathways\",\"authors\":\"\",\"doi\":\"10.1016/j.algal.2024.103756\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The current research looked into the inhibitory effect of a purified component of <em>Sargassum hornery</em> fucoidan oligosaccharide (F2) with a sulfate content of 16.35 %, which showed substantial protective effects on H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in RAW 264.7 cells and zebrafish. The chemical and structural characteristics were examined by GC, FTIR, MS, and NMR spectroscopy, revealing that F2 was composed of not only a mixed oligosaccharide with 1–4 degrees of polymerization but also had a main chain consisting of (1 → 3) linked α-L-Fuc<em>p</em> and (1 → 3,4) linked α-L-Fuc<em>p</em>, sulfated groups mainly at C2 position. It was found that F2 could activate the MAPK and Keap1-Nrf2 signaling pathways through the dissociation of the Keap1-Nrf2 complex in RAW 264.7 cells. Then Nrf2 entered the cell and bound to the antioxidant response element (ARE), resulting in activating the expression of downstream antioxidant genes. F2 was also found to have a strong protective effect on oxidative stress in zebrafish induced by H<sub>2</sub>O<sub>2</sub>, which can reduce the heart rate of zebrafish and prevent the generation of intracellular ROS and cell death. Therefore, <em>S. hornery</em> fucoidan oligosaccharide (F2) could be a potential ingredient for functional foods, cosmetics, and pharmaceuticals because of its antioxidant activity.</div></div>\",\"PeriodicalId\":7855,\"journal\":{\"name\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-10-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2211926424003680\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2211926424003680","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Sargassum hornery fucoidan oligosaccharide: Purification, characterization, and antioxidant effects targeting the MAPK and KEAP1-NRF2 signaling pathways
The current research looked into the inhibitory effect of a purified component of Sargassum hornery fucoidan oligosaccharide (F2) with a sulfate content of 16.35 %, which showed substantial protective effects on H2O2-induced oxidative damage in RAW 264.7 cells and zebrafish. The chemical and structural characteristics were examined by GC, FTIR, MS, and NMR spectroscopy, revealing that F2 was composed of not only a mixed oligosaccharide with 1–4 degrees of polymerization but also had a main chain consisting of (1 → 3) linked α-L-Fucp and (1 → 3,4) linked α-L-Fucp, sulfated groups mainly at C2 position. It was found that F2 could activate the MAPK and Keap1-Nrf2 signaling pathways through the dissociation of the Keap1-Nrf2 complex in RAW 264.7 cells. Then Nrf2 entered the cell and bound to the antioxidant response element (ARE), resulting in activating the expression of downstream antioxidant genes. F2 was also found to have a strong protective effect on oxidative stress in zebrafish induced by H2O2, which can reduce the heart rate of zebrafish and prevent the generation of intracellular ROS and cell death. Therefore, S. hornery fucoidan oligosaccharide (F2) could be a potential ingredient for functional foods, cosmetics, and pharmaceuticals because of its antioxidant activity.
期刊介绍:
Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment