Selective and sensitive electroanalytical biosensor based on Poly([Cu(H2O)2P2]I2)/GCE for the determination of esomeprazole in pharmaceutical formulations and biological fluid samples

The need to determine esomeprazole in pharmaceutical formulations and biological fluids with high accuracy arises to ensure proper dosage and follow-up on patient response. In the present investigation, Glassy Carbon Electrode (GCE) was modified by potentiodynamic electropolymerization of Diaquabis(1,10-phenanthroline copper (II) Iodide ([Cu(H₂O)₂P₂]I₂) on its surface with a fixed number of cycles. Cyclic voltammetry (CV) was used to characterize the voltammetric redox behavior of esomeprazole (ESO) on poly([Cu(H₂O)₂P₂]I₂)/GCE and the best reversible oxidative current response has been obtained. On the proposed electrode, the electrochemical activity of the ESO was enhanced at a peak intensity that was around 2.6 times higher than the bare one. The calibration curve for ESO at poly([Cu(H₂O)₂P₂]I₂)/GCE has a linear region for a concentration range of 0.01–200 µM with the detection limit and limit of quantifications of 28.90 and 95.53, respectively and the associated standard deviation was 0.59 %. he current sensor has also been effectively used to detect esomeprazole in clinical fluid samples and pharmaceutical samples in a sensitive and selective approach. The outstanding recovery percentage in the range of 95.97–105.26 % in the presence of 50–300 % potential interferents indicated an excellent accuracy and selectivity of the proposed sensor towards ESO in the presence of potential interferants. The proposed method is simple, quick, stable, and effective for real sensor applications for esomeprazole sensing in different samples.