利用凝胶电泳分离荧光标记的短肽，进行体外转化研究

IF 0.8 Q3 Engineering Nanotechnologies in Russia Pub Date : 2024-10-29 DOI:10.1134/S263516762460127X

O. A. Tolicheva, M. S. Bidzhieva, P. S. Kasatskiy, V. I. Marina, P. V. Sergiev, A. L. Konevega, A. Paleskava

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引用次数: 0

摘要

开发简单、易用、灵敏的细菌蛋白质合成研究方法是一项重要的基础和应用任务。最近，我们证明了在变性条件下使用尿素-聚丙烯酰胺凝胶电泳观察含 BODIPY 标记的苯丙氨酸和亮氨酸短肽的可能性。在这项工作中，我们扩大了测试序列的范围，包括含有缬氨酸、赖氨酸和修饰氨基酸εNH2-DOTA-赖氨酸的肽。所述方法也可用于研究翻译抑制的机制，如对抗生素乙胺霉素 A 和紫霉素特异性作用的阐述所示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Study

The development of simple, readily available, and sensitive methods for studying bacterial protein synthesis is an important fundamental and applied task. We recently demonstrated the possibility of visualizing short BODIPY-labeled phenylalanine- and leucine-containing peptides using urea-polyacrylamide gel electrophoresis under denaturing conditions. In this work, we expand the range of test sequences and included peptides with valine, lysine, and the modified amino acid εNH₂-DOTA-lysine. The described method can also be used to study the mechanism of translation inhibition, as shown by elaboration of the specific action of the antibiotics etamycin A and viomycin.

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来源期刊

Nanotechnologies in Russia NANOSCIENCE & NANOTECHNOLOGY-

CiteScore

1.20

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0.00%

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期刊介绍： Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.