提高日粮纳米配方槲皮素对蛋鸡产蛋性能的影响:鸡蛋质量、储存鸡蛋的氧化稳定性、肠道免疫和抗氧化剂相关基因的表达。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-10-29 DOI:10.1186/s12917-024-04327-x
Hanan S Al-Khalaifah, Doaa Ibrahim, Asmaa El-Sayed Kamel, Afaf Al-Nasser, Abdelwahab A Abdelwarith, Elshimaa M Roushdy, Nagwa I Sheraiba, Basant M Shafik, Sara M El-Badry, Elsayed M Younis, Maha Mamdouh, Engy Mohamed Mohamed Yassin, Simon J Davies, Asmaa T Y Kishawy
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引用次数: 0

摘要

背景:使用天然抗氧化剂进行营养干预可为改变母鸡的生产性能和保持鸡蛋在储存期间的氧化稳定性提供实用的解决方案。槲皮素是最丰富的黄酮类化合物,具有强大的抗氧化和免疫刺激活性。将槲皮素这种强效抗氧化剂和免疫刺激剂融入有效的纳米载体(QNPs)的概念提高了其生物利用率和稳定性,因此,我们首次评估了其对蛋鸡的生产性能、免疫力和鸡蛋在储存期间的质量的影响。将 400 只 12 周龄的褐线蛋鸡分成 4 个实验组:对照组饲喂基础日粮,其他 3 组饲喂添加了 100、200 和 300 毫克/千克 QNPs 的基础日粮,为期 60 周:结果:QNPs200 和 QNPs300 可提高产蛋率、蛋重百分比、蛋重和蛋黄重量,从而改善产蛋性能和蛋的质量。添加 QNPs300 能显著降低蛋鸡血清总胆固醇,同时减少蛋黄中的饱和脂肪酸和胆固醇,而增加多不饱和脂肪酸。在 45 天的储存期间,QNPs 提高了鸡蛋中的磷脂、总酚类和类黄酮、总抗氧化活性(T-AOC),同时降低了 MDA 含量。此外,饲喂 QNPs 的母鸡血清和肠道中的免疫反应增强,表现为溶菌酶活性、IgM、IgG 和吞噬指数升高,NO 下降,AVBD 6-12、IL-10、IgM 和 ATg 5-7-12 上调,IL-1β 和 TNF-α 下调,尤其是 QNPs200 和 300。在喂食较高剂量 QNPs 的组别中,肠道氧化还原平衡得到改善,H2O2 和 MDA 下降,同时过氧化氢酶、SOD、GSH-Px、HO-1 和 NQO1 上调:补充 QNPs 提供了一种新的营养策略,可提高母鸡的生产性能,为鸡蛋添加天然抗氧化剂,防止鸡蛋在储存过程中质量下降。
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Enhancing impact of dietary nano formulated quercetin on laying performance: egg quality, oxidative stability of stored eggs, intestinal immune and antioxidants related genes expression.

Background: Nutritional interventions with natural antioxidants can provide a pragmatic solution for modifying hens' performance and maintaining oxidative stability of eggs during storage. Quercetin is the most abundant flavonoids with potent antioxidant and immune stimulant activities. The concept of incorporating of quercetin, as potent antioxidant and immunostimulant, into effective nano-carriers (QNPs) has promoted their bioavailability and stability thus, their effectiveness for the first time were assessed on laying hens' performance and immunity, eggs quality during storage. Four hundred 12-weeks-old Hy-line brown laying hens were distributed to four experimental groups: control group fed basal diets, and other 3 groups fed basal diets fortified with 100, 200 and 300 mg/kg QNPs for 60 weeks.

Results: Laying performance and quality of laid eggs were improved as expressed by elevated laying rate, egg mass %, eggs weight and yolk weight in QNPs200 and 300. Fortification of QNPs300 remarkably decreased layers serum total cholesterol concurrently with decreased egg yolk saturated fatty acids and cholesterol while increased polyunsaturated fatty acids. Over- 45 days storage period, QNPs enhanced phospholipids, total phenolics and flavonoids, total antioxidant activity (T-AOC) simultaneous with decreased MDA content in eggs. Furthermore, enhanced immune response was detected in both in serum and intestine of QNPs fed hens as reflected by higher lysozymes activity, IgM, IgG and phagocytic index and demotion of NO together with AvBD 6-12, IL-10, IgM and ATg 5-7-12 upregulation and downregulation of IL-1β and TNF-α especially at QNPs200 and 300. Intestinal redox balance was modified via decreasing H2O2 and MDA simultaneous with upregulation of catalase, SOD, GSH-Px, HO-1 and NQO1 in groups fed higher doses of QNPs.

Conclusions: QNPs supplementation provides a new nutritional strategy towards increasing hen performance, fortification of eggs with natural antioxidants that prevents egg quality deterioration during storage.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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