微生物重组蛋白生产和功能肽标签方面的进展。

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-30 DOI:10.1093/bbb/zbae147

Teruyo Ojima-Kato

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引用次数: 0

摘要

在原核和真核细胞中生产重组蛋白质是科研和工业领域的一项基础技术。实现高效蛋白质合成是加速蛋白质发现、表征和实际应用的关键。本综述重点介绍重组蛋白质生产的最新进展以及提高蛋白质生产效率的策略，尤其是利用大肠杆菌和酿酒酵母生产蛋白质的策略。此外，本综述还总结了可用于蛋白质生产、修饰和纯化的各种功能肽标签的开发情况，包括我们研究小组开发的翻译增强肽标签。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Advances in recombinant protein production in microorganisms and functional peptide tags.

Recombinant protein production in prokaryotic and eukaryotic cells is a fundamental technology for both research and industry. Achieving efficient protein synthesis is key to accelerating the discovery, characterization, and practical application of proteins. This review focuses on recent advances in recombinant protein production and strategy for more efficient protein production especially using Escherichia coli and Saccharomyces cerevisiae. Additionally, this review summarizes the development of various functional peptide tags that can be employed for protein production, modification, and purification, including translation enhancing peptide tags developed by our research group.

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).