膳食碳水化合物可调节变异链球菌的黏附性和细菌蛋白质组。

IF 2.9 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Caries Research Pub Date : 2024-10-30 DOI:10.1159/000541821
Lina M Marin, Yizhi Xiao, Jin Seo, Daniel Queiroz, Walter L Siqueira
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引用次数: 0

摘要

导言:变异链球菌对牙齿表面的粘附以及随后的生物膜形成受碳水化合物来源的调节,但对细菌蛋白质组的相应影响以前还没有研究过。本研究旨在评估不同碳水化合物在两个时间点(早期附着(8 小时)和生物膜成熟(24 小时))对变异单胞菌存活率和细菌蛋白质组的影响。方法:将涂有腮腺唾液蛋白的羟基磷灰石(HAp)圆片与变异单胞菌 UA159 一起接种在不含葡萄糖的胰蛋白胨大豆肉汤中,并补充以下一种碳水化合物(n=12/处理/时间点):1%蔗糖 (S);0.525% 葡萄糖 + 0.525% 果糖 (G+F);10% 木糖醇 (X);10% 木糖醇 + 1%蔗糖 (X+S);或不添加任何物质的培养基作为阴性对照 (C)。接种后,HAp 盘在 37 °C 和 10% CO2 下培养 8 或 24 小时。每个培养期结束后,用平板计数法对 6 个 HAp 圆片/组的附着细菌进行定量,然后用剩余的 6 个 HAp 圆片/组提取细菌细胞壁蛋白。提取的蛋白质采用液相色谱-质谱法进行分析,然后按其生物过程进行分类。研究分三个独立的实验进行,在每个时间点测定附着在 HAp 盘上的细菌数量,并通过双向方差分析和 Bonferroni 检验进行分析(=5%):结果表明:木糖醇在8小时和24小时内明显抑制细菌的粘附和代谢;然而,当木糖醇与蔗糖结合时,细菌的粘附和代谢明显增强,在两个时间点上对变异单胞菌均无负面影响。细菌蛋白质组受碳水化合物来源的影响:结论:替代甜味剂木糖醇可降低变异沙雷氏菌生物膜的致龋性;但与可发酵糖类结合使用可能会抑制这种有益效果。
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Dietary carbohydrates modulate Streptococcus mutans adherence and bacterial proteome.

Introduction: Streptococcus mutans adherence to the tooth surface and subsequent biofilm development is modulated by the carbohydrate source, but the corresponding effect on bacterial proteome has not been previously studied. This study aimed to assess the effect of different carbohydrates on S. mutans viability and bacterial proteome at two-time points, early attachment (8 h) and biofilm maturation (24 h).

Methods: Hydroxyapatite (HAp) discs coated with parotid saliva proteins were inoculated with S. mutans UA159 in Tryptone Soy Broth without dextrose supplemented with one of the following carbohydrates (n=12/treatment/time point): 1% Sucrose (S); 0.525% Glucose + 0.525% Fructose (G+F); 10% Xylitol (X); 10% Xylitol + 1% Sucrose (X+S); or culture medium without supplementation as negative control (C). Once inoculated, HAp discs were incubated for 8 h or 24 h at 37 °C and 10% CO2. After each incubation period, adhered bacteria were quantified using the plate-counting method for 6 HAp discs/group, and the remaining 6 HAp discs/group were used to extract bacterial cell wall proteins. Extracted proteins were analyzed using liquid chromatography coupled with mass spectrometry and then classified by their biological process. The study was conducted in three independent assays and the number of bacteria adhered to the HAp discs was determined at each time point and analyzed by two-way ANOVA followed by Bonferroni test (=5%).

Results: The results suggest that xylitol significantly repressed bacterial adherence and metabolism at 8 h and 24 h; however, bacterial adherence and metabolism were significantly enhanced when xylitol was combined with sucrose, showing no negative effect on S. mutans at both time points. Bacterial proteome was modulated by the carbohydrate source.

Conclusion: The cariogenicity of S. mutans biofilms may be reduced by the alternative sweetener xylitol; however, the combination with fermentable sugars may inhibit such a beneficial effect.

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来源期刊
Caries Research
Caries Research 医学-牙科与口腔外科
CiteScore
6.30
自引率
7.10%
发文量
34
审稿时长
6-12 weeks
期刊介绍: ''Caries Research'' publishes epidemiological, clinical and laboratory studies in dental caries, erosion and related dental diseases. Some studies build on the considerable advances already made in caries prevention, e.g. through fluoride application. Some aim to improve understanding of the increasingly important problem of dental erosion and the associated tooth wear process. Others monitor the changing pattern of caries in different populations, explore improved methods of diagnosis or evaluate methods of prevention or treatment. The broad coverage of current research has given the journal an international reputation as an indispensable source for both basic scientists and clinicians engaged in understanding, investigating and preventing dental disease.
期刊最新文献
Dietary carbohydrates modulate Streptococcus mutans adherence and bacterial proteome. Effectiveness of a Sugar Consumption Prevention Program in the First Year of Life on the Occurrence of Early Childhood Caries: A Multicentric Randomized Trial in Brazil. Deep learning-based algorithm for staging secondary caries in bitewings. Hydroxyapatite in oral care products: in vitro effects on erosion/abrasion and analysis of formulation components. Dental caries among over 1.5 million records of schoolchildren in Singapore, 2007 - 2019: age-period-cohort effect analyses.
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