解读毛霉菌(Tv1)对番茄镰刀菌-线虫复合萎蔫病的生物防治潜力

IF 3.5 4区 生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-10-29 DOI:10.1002/jobm.202400595
Selva A Anbalagan, Sudha Appusamy, Parthiban V Kumaresan, Gopalakrishnan Chellappan, Swarnakumari Narayanan, Anandham Rangasamy, Kahkashan Perveen, Najat A Bukhari, Riyaz Sayyed
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引用次数: 0

摘要

该研究评估了毛霉菌作为生物控制剂管理番茄作物枯萎线虫复合体的情况,旨在减少产量损失。从受感染的植物样本中分离出 Fusarium sp.和 Meloidogyne sp.,并从分子和形态上确认为 Fusarium oxysporum f.sp. lycopersici 和 Meloidogyne incognita。利用 ITS 和 TEF1 引物对从印度泰米尔纳德邦农业大学植物病理学系获得的四种毛霉菌属进行了分子鉴定,分别为赤霉菌(Tv1)、赤霉菌(Tasd1)、哈茨真菌(Th1)和科宁氏毛霉菌(Tk1)。其中,Tv1 能有效抑制病原菌分离物的菌丝生长。此外,Tv1 的粗代谢物也有类似的效果。培养 24-72 h 后,M. incognita J2s 的死亡率为 90.48% 至 100%,卵孵化抑制率达到 90.20%。用 Tv1 分生孢子处理的番茄植株的芽长、根长、鲜重和干重都有所增加。在盆栽实验中,Tv1 处理可降低病害发生率 64.57%,与多菌灵处理(82.32%)相当。与接种后未处理的对照植物相比,Tv1 处理过的植物每 100 克根中的根瘿、卵块和 J2 更少。经多菌灵(1.98 ± 0.047)和 Tv1(3.06 ± 0.086)处理的植株的根结指数(RKI)明显低于对照植株(4.47 ± 0.109)。Tv1 对 M. incognita 的生物防治效率为 21.04%,Tv1 处理组的线虫繁殖系数(RF)降至 0.53。根据上述研究结果,Tv1 在土壤中施用可有效控制线虫数量和枯萎病。
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Deciphering the Biocontrol Potential of Trichoderma asperellum (Tv1) Against Fusarium-Nematode Wilt Complex in Tomato.

The study assessed Trichoderma spp. as a biocontrol agent for managing wilt-nematode complex in tomato crops, aiming to mitigate yield losses. Fusarium sp. and Meloidogyne sp. were isolated from infected plant samples and confirmed molecularly and morphologically as Fusarium oxysporum f.sp. lycopersici and Meloidogyne incognita. Four Trichoderma spp. procured from the Department of Plant Pathology, Tamil Nadu Agricultural University, India were identified molecularly as Trichoderma asperellum (Tv1), Trichoderma asperelloides (Tasd1), Trichoderma harzianum (Th1), and Trichoderma koningiopsis (Tk1) utilizing ITS and TEF1 primer pairs. Among them, Tv1 effectively inhibited the mycelial growth of pathogen isolates. Furthermore, crude metabolite of Tv1 exhibited similar effects. The mortality rate of M. incognita J2s ranged from 90.48% to 100% after 24-72 h of incubation and inhibition percentage of egg hatching reached 90.20%. The shoot length, root length, fresh weight, and dry weight of the tomato plants treated with Tv1 conidia were increased. In a pot experiment, Tv1 treatment reduced disease incidence by 64.57%, comparable to carbendazim treatment (82.32%). Tv1-treated plants had fewer root galls, egg masses, and J2s per 100 g of roots than the inoculated-untreated control plants. The root-knot index (RKI) was significantly lower in plants treated with carbafuran (1.98 ± 0.047) and Tv1 (3.06 ± 0.086) than in control (4.47 ± 0.109). The bio-control efficiency of Tv1 against M. incognita was 21.04%, and the nematodes' reproductive factor (RF) declined to 0.53 in Tv1 treatment group. Based on the findings above, it was established that Tv1 effectively controlled nematode populations and wilt disease when applied in soil.

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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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