传染性法氏囊病病毒 VP5 的单克隆抗体开发和抗原表位鉴定。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Veterinary journal Pub Date : 2024-10-29 DOI:10.1016/j.tvjl.2024.106254
Jinze Han , Xinxin Niu , Chengfei Ge , Ziwen Wu , Guodong Wang , Mengmeng Huang , Yulong Zhang , Runhang Liu , Mengmeng Xu , Hangbo Yu , Jingzhe Han , Suyan Wang , Yongzhen Liu , Yuntong Chen , Hongyu Cui , Yanping Zhang , Yulu Duan , Xiaomei Wang , Liuan Li , Yulong Gao , Xiaole Qi
{"title":"传染性法氏囊病病毒 VP5 的单克隆抗体开发和抗原表位鉴定。","authors":"Jinze Han ,&nbsp;Xinxin Niu ,&nbsp;Chengfei Ge ,&nbsp;Ziwen Wu ,&nbsp;Guodong Wang ,&nbsp;Mengmeng Huang ,&nbsp;Yulong Zhang ,&nbsp;Runhang Liu ,&nbsp;Mengmeng Xu ,&nbsp;Hangbo Yu ,&nbsp;Jingzhe Han ,&nbsp;Suyan Wang ,&nbsp;Yongzhen Liu ,&nbsp;Yuntong Chen ,&nbsp;Hongyu Cui ,&nbsp;Yanping Zhang ,&nbsp;Yulu Duan ,&nbsp;Xiaomei Wang ,&nbsp;Liuan Li ,&nbsp;Yulong Gao ,&nbsp;Xiaole Qi","doi":"10.1016/j.tvjl.2024.106254","DOIUrl":null,"url":null,"abstract":"<div><div>Infectious bursal disease (IBD) is an important immunosuppressive disease affecting chickens and is caused by infectious bursal disease virus (IBDV) infection. VP5 is a non-essential protein for IBDV replication but plays a critical role in IBDV pathogenesis. A deeper understanding of the biological functions of VP5 is lacking. This study utilized a prokaryotic system to express and purify soluble VP5 from the dominant epidemic strain of IBDV and developed a hybridoma cell line capable of secreting IBDV VP5 monoclonal antibody (MAb). The VP5 MAb demonstrated strong specificity for IBDV VP5 and could effectively discriminate between IBDV and its VP5-deleted strain. Furthermore, the antigen epitope of 137RRDLPKPE145 from IBDV VP5 was identified, which is the first detailed report of an IBDV VP5 antigen epitope. This antigen epitope, which is located at the C-terminus of VP5, is conserved across various IBDV serotype 1 strains. The findings of this study offer valuable insights for further exploration of gene function and differential detection of VP5.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"308 ","pages":"Article 106254"},"PeriodicalIF":2.3000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5\",\"authors\":\"Jinze Han ,&nbsp;Xinxin Niu ,&nbsp;Chengfei Ge ,&nbsp;Ziwen Wu ,&nbsp;Guodong Wang ,&nbsp;Mengmeng Huang ,&nbsp;Yulong Zhang ,&nbsp;Runhang Liu ,&nbsp;Mengmeng Xu ,&nbsp;Hangbo Yu ,&nbsp;Jingzhe Han ,&nbsp;Suyan Wang ,&nbsp;Yongzhen Liu ,&nbsp;Yuntong Chen ,&nbsp;Hongyu Cui ,&nbsp;Yanping Zhang ,&nbsp;Yulu Duan ,&nbsp;Xiaomei Wang ,&nbsp;Liuan Li ,&nbsp;Yulong Gao ,&nbsp;Xiaole Qi\",\"doi\":\"10.1016/j.tvjl.2024.106254\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Infectious bursal disease (IBD) is an important immunosuppressive disease affecting chickens and is caused by infectious bursal disease virus (IBDV) infection. VP5 is a non-essential protein for IBDV replication but plays a critical role in IBDV pathogenesis. A deeper understanding of the biological functions of VP5 is lacking. This study utilized a prokaryotic system to express and purify soluble VP5 from the dominant epidemic strain of IBDV and developed a hybridoma cell line capable of secreting IBDV VP5 monoclonal antibody (MAb). The VP5 MAb demonstrated strong specificity for IBDV VP5 and could effectively discriminate between IBDV and its VP5-deleted strain. Furthermore, the antigen epitope of 137RRDLPKPE145 from IBDV VP5 was identified, which is the first detailed report of an IBDV VP5 antigen epitope. This antigen epitope, which is located at the C-terminus of VP5, is conserved across various IBDV serotype 1 strains. The findings of this study offer valuable insights for further exploration of gene function and differential detection of VP5.</div></div>\",\"PeriodicalId\":23505,\"journal\":{\"name\":\"Veterinary journal\",\"volume\":\"308 \",\"pages\":\"Article 106254\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-10-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S109002332400193X\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary journal","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S109002332400193X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

传染性法氏囊病(IBD)是一种重要的鸡免疫抑制疾病,由传染性法氏囊病病毒(IBDV)感染引起。VP5 是 IBDV 复制的非必需蛋白,但在 IBDV 发病机制中起着关键作用。目前还缺乏对 VP5 生物功能的深入了解。本研究利用原核系统表达和纯化了 IBDV 优势流行株的可溶性 VP5,并开发了一种能分泌 IBDV VP5 单克隆抗体(MAb)的杂交瘤细胞系。VP5 MAb 对 IBDV VP5 有很强的特异性,能有效区分 IBDV 及其 VP5 缺失株。此外,还鉴定出了 IBDV VP5 的抗原表位 137RRDLPKPE145,这是首次详细报道 IBDV VP5 抗原表位。该抗原表位位于 VP5 的 C 端,在不同的 IBDV 血清 1 型毒株中具有一致性。这项研究的发现为进一步探索基因功能和 VP5 的差异检测提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5
Infectious bursal disease (IBD) is an important immunosuppressive disease affecting chickens and is caused by infectious bursal disease virus (IBDV) infection. VP5 is a non-essential protein for IBDV replication but plays a critical role in IBDV pathogenesis. A deeper understanding of the biological functions of VP5 is lacking. This study utilized a prokaryotic system to express and purify soluble VP5 from the dominant epidemic strain of IBDV and developed a hybridoma cell line capable of secreting IBDV VP5 monoclonal antibody (MAb). The VP5 MAb demonstrated strong specificity for IBDV VP5 and could effectively discriminate between IBDV and its VP5-deleted strain. Furthermore, the antigen epitope of 137RRDLPKPE145 from IBDV VP5 was identified, which is the first detailed report of an IBDV VP5 antigen epitope. This antigen epitope, which is located at the C-terminus of VP5, is conserved across various IBDV serotype 1 strains. The findings of this study offer valuable insights for further exploration of gene function and differential detection of VP5.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinary journal
Veterinary journal 农林科学-兽医学
CiteScore
4.10
自引率
4.50%
发文量
79
审稿时长
40 days
期刊介绍: The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.
期刊最新文献
Assessment of Brain Injury in Cattle with Theileria annulata: Neuron-Specific Biomarkers, Inflammation and Apoptosis. BRSV seroprevalence and associated risk factors on Dutch dairy farms. Rabbit teeth serve as a calcium source for rabbits (Oryctolagus cuniculus). The effect of peri- and postoperative antibiotic prophylaxis on surgical site infection in surgeries with elective antibiotic administration. The problem that residual Mycobacterium bovis infection poses for the eradication of bovine tuberculosis
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1