{"title":"异构体钴基金属有机框架和还原氧化石墨烯纳米复合材料用于在 HPLC-PDA 前从早产儿呼出气体冷凝物样品中分散固相萃取咖啡因","authors":"","doi":"10.1016/j.jchromb.2024.124339","DOIUrl":null,"url":null,"abstract":"<div><div>A cobalt-based metal–organic framework and graphene oxide were combined to prepare a new nanocomposite for extracting of caffeine from exhaled breath condensate (EBC) samples. Dispersive micro solid phase extraction of caffeine was conducted using the nanocomposite as a sorbent by adding 10 mg of it to the sample solution and vortexing for 3 min. After extracting of the analyte, it was eluted using the mobile phase. The analyte was then analyzed using high performance liquid chromatography-photodiode array detector. Under optimal conditions, the limit of detection, limit of quantification, and linear range of the calibration curve were found to be 1.7, 5.9, and 10–500 µg/L, respectively. To assess the precision of the method, five replicates of standard solutions containing caffeine at two different concentration levels (50 and 100 µg/L) were tested. The relative standard deviations for intra- and inter-day precisions ranged from 4.3 to 6.8 %. The applicability of the method was demonstrated by analyzing the samples obtained from premature infants undergoing caffeine treatment and caffeine concentrations were 4.9 ± 0.6, 2.7 ± 0.2 µg/L in the EBC samples of who were under treatment by a 5-mg dose. Also, caffeine concentrations were 5.9 ± 0.3 and 18 ± 0.6 µg/L in the the infants who obtained the 10-mg and 25-mg doses, respectively. The results indicated a satisfactory, extraction recovery of 86 % showcasing the method’s reliability and effectiveness in analyzing real samples.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Heteroatom cobalt-based metal-organic framework and reduced graphene oxide nanocomposite for dispersive solid phase extraction of caffeine from exhaled breath condensate samples of premature infants prior to HPLC-PDA\",\"authors\":\"\",\"doi\":\"10.1016/j.jchromb.2024.124339\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>A cobalt-based metal–organic framework and graphene oxide were combined to prepare a new nanocomposite for extracting of caffeine from exhaled breath condensate (EBC) samples. Dispersive micro solid phase extraction of caffeine was conducted using the nanocomposite as a sorbent by adding 10 mg of it to the sample solution and vortexing for 3 min. After extracting of the analyte, it was eluted using the mobile phase. The analyte was then analyzed using high performance liquid chromatography-photodiode array detector. Under optimal conditions, the limit of detection, limit of quantification, and linear range of the calibration curve were found to be 1.7, 5.9, and 10–500 µg/L, respectively. To assess the precision of the method, five replicates of standard solutions containing caffeine at two different concentration levels (50 and 100 µg/L) were tested. The relative standard deviations for intra- and inter-day precisions ranged from 4.3 to 6.8 %. The applicability of the method was demonstrated by analyzing the samples obtained from premature infants undergoing caffeine treatment and caffeine concentrations were 4.9 ± 0.6, 2.7 ± 0.2 µg/L in the EBC samples of who were under treatment by a 5-mg dose. Also, caffeine concentrations were 5.9 ± 0.3 and 18 ± 0.6 µg/L in the the infants who obtained the 10-mg and 25-mg doses, respectively. The results indicated a satisfactory, extraction recovery of 86 % showcasing the method’s reliability and effectiveness in analyzing real samples.</div></div>\",\"PeriodicalId\":348,\"journal\":{\"name\":\"Journal of Chromatography B\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Chromatography B\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1570023224003489\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography B","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1570023224003489","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Heteroatom cobalt-based metal-organic framework and reduced graphene oxide nanocomposite for dispersive solid phase extraction of caffeine from exhaled breath condensate samples of premature infants prior to HPLC-PDA
A cobalt-based metal–organic framework and graphene oxide were combined to prepare a new nanocomposite for extracting of caffeine from exhaled breath condensate (EBC) samples. Dispersive micro solid phase extraction of caffeine was conducted using the nanocomposite as a sorbent by adding 10 mg of it to the sample solution and vortexing for 3 min. After extracting of the analyte, it was eluted using the mobile phase. The analyte was then analyzed using high performance liquid chromatography-photodiode array detector. Under optimal conditions, the limit of detection, limit of quantification, and linear range of the calibration curve were found to be 1.7, 5.9, and 10–500 µg/L, respectively. To assess the precision of the method, five replicates of standard solutions containing caffeine at two different concentration levels (50 and 100 µg/L) were tested. The relative standard deviations for intra- and inter-day precisions ranged from 4.3 to 6.8 %. The applicability of the method was demonstrated by analyzing the samples obtained from premature infants undergoing caffeine treatment and caffeine concentrations were 4.9 ± 0.6, 2.7 ± 0.2 µg/L in the EBC samples of who were under treatment by a 5-mg dose. Also, caffeine concentrations were 5.9 ± 0.3 and 18 ± 0.6 µg/L in the the infants who obtained the 10-mg and 25-mg doses, respectively. The results indicated a satisfactory, extraction recovery of 86 % showcasing the method’s reliability and effectiveness in analyzing real samples.
期刊介绍:
The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis.
Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches.
Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.