{"title":"基于 D-π-A 结构的近红外开启荧光探针在小鼠肾缺血再灌注损伤中的半胱氨酸成像研究","authors":"Chunpo Ge, Feng Pei, Pengcheng Zhang, Xiaoyu Wang, Xiaopeng Jiang, Kaiwen Chang, Zhijun Yang","doi":"10.1016/j.jphotochem.2024.116114","DOIUrl":null,"url":null,"abstract":"<div><div>Cysteine (Cys) is an important biomarker, particularly relevant in studying physiological and pathological processes related to oxidative stress. Small-molecule fluorescent probes for detecting Cys are essential in these investigations. In this study, we designed and synthesized the fluorescent probe DCI-Th-Cys, utilizing dicyanoisophorone and acrylic ester as its structural components. DCI-Th-Cys exhibits near-infrared emission at 670 nm, with a large Stokes shift of 190 nm upon reacting with Cys, demonstrating high sensitivity and selectivity. We successfully imaged Cys in both cellular and mouse models, notably detecting elevated Cys levels in the kidneys of a mouse model with renal ischemia–reperfusion injury.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"460 ","pages":"Article 116114"},"PeriodicalIF":4.1000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Based on D–π–A structure of near-infrared turn-on fluorescent probe for cysteine imaging in renal ischemia–reperfusion injury in mice\",\"authors\":\"Chunpo Ge, Feng Pei, Pengcheng Zhang, Xiaoyu Wang, Xiaopeng Jiang, Kaiwen Chang, Zhijun Yang\",\"doi\":\"10.1016/j.jphotochem.2024.116114\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Cysteine (Cys) is an important biomarker, particularly relevant in studying physiological and pathological processes related to oxidative stress. Small-molecule fluorescent probes for detecting Cys are essential in these investigations. In this study, we designed and synthesized the fluorescent probe DCI-Th-Cys, utilizing dicyanoisophorone and acrylic ester as its structural components. DCI-Th-Cys exhibits near-infrared emission at 670 nm, with a large Stokes shift of 190 nm upon reacting with Cys, demonstrating high sensitivity and selectivity. We successfully imaged Cys in both cellular and mouse models, notably detecting elevated Cys levels in the kidneys of a mouse model with renal ischemia–reperfusion injury.</div></div>\",\"PeriodicalId\":16782,\"journal\":{\"name\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"volume\":\"460 \",\"pages\":\"Article 116114\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1010603024006580\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Photochemistry and Photobiology A-chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1010603024006580","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Based on D–π–A structure of near-infrared turn-on fluorescent probe for cysteine imaging in renal ischemia–reperfusion injury in mice
Cysteine (Cys) is an important biomarker, particularly relevant in studying physiological and pathological processes related to oxidative stress. Small-molecule fluorescent probes for detecting Cys are essential in these investigations. In this study, we designed and synthesized the fluorescent probe DCI-Th-Cys, utilizing dicyanoisophorone and acrylic ester as its structural components. DCI-Th-Cys exhibits near-infrared emission at 670 nm, with a large Stokes shift of 190 nm upon reacting with Cys, demonstrating high sensitivity and selectivity. We successfully imaged Cys in both cellular and mouse models, notably detecting elevated Cys levels in the kidneys of a mouse model with renal ischemia–reperfusion injury.
期刊介绍：
JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds.
All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor).
The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.
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