Xin Liu , Tianqi Shi , Jiaming Li , Huanyu Wu , Qing Zhao , Zhixin Fang , Yingying Liang , Quan Xiao , Min Chen , Qingli Dong , Hongzhi Zhang
{"title":"pLM33 使持久性单核细胞增多性李斯特菌 ST5 能够耐受各种应激条件,并增强其毒力","authors":"Xin Liu , Tianqi Shi , Jiaming Li , Huanyu Wu , Qing Zhao , Zhixin Fang , Yingying Liang , Quan Xiao , Min Chen , Qingli Dong , Hongzhi Zhang","doi":"10.1016/j.fm.2024.104675","DOIUrl":null,"url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> is a major foodborne pathogen. In our previous study, we found that <em>L. monocytogenes</em> ST5 and ST121 strains were dominant in two food plants of Shanghai. Genetic characterization revealed that the environmental tolerance of these strains was attributable to the plasmids pLM33 and pLM5578. To further evaluate the function of <em>L. monocytogenes</em> plasmids<em>,</em> we selected ST5 and ST121 wild-type strains, and used their plasmid-cured strains as controls to conduct tolerance tests. In addition, we analyzed 108 <em>L. monocytogenes</em> strains isolated from four major food categories in Shanghai. Our results showed that compared with the plasmid-cured strain, the ST5 strain carrying pLM33 showed higher tolerance to environmental stress conditions, including low acid, high salt, oxidizing, and high-temperature conditions; as well as higher virulence. Furthermore, we found that the plasmid carriage rate of food isolates was 97.22%, with the highest carriage rate of 68.57% for pLM5578, followed by 24.76% for pLM33. Notably, all <em>L. monocytogenes</em> ST5 isolates from ready-to-eat food products (n = 11) carried plasmids, suggesting that contamination of these food products may pose a serious risk to human health. In summary, the results of this study broaden our understanding regarding the role of <em>L. monocytogenes</em> plasmids in stress responses.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"126 ","pages":"Article 104675"},"PeriodicalIF":4.5000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"pLM33 provides tolerance of persistent Listeria monocytogenes ST5 to various stress conditions and also enhances its virulence\",\"authors\":\"Xin Liu , Tianqi Shi , Jiaming Li , Huanyu Wu , Qing Zhao , Zhixin Fang , Yingying Liang , Quan Xiao , Min Chen , Qingli Dong , Hongzhi Zhang\",\"doi\":\"10.1016/j.fm.2024.104675\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Listeria monocytogenes</em> is a major foodborne pathogen. In our previous study, we found that <em>L. monocytogenes</em> ST5 and ST121 strains were dominant in two food plants of Shanghai. Genetic characterization revealed that the environmental tolerance of these strains was attributable to the plasmids pLM33 and pLM5578. To further evaluate the function of <em>L. monocytogenes</em> plasmids<em>,</em> we selected ST5 and ST121 wild-type strains, and used their plasmid-cured strains as controls to conduct tolerance tests. In addition, we analyzed 108 <em>L. monocytogenes</em> strains isolated from four major food categories in Shanghai. Our results showed that compared with the plasmid-cured strain, the ST5 strain carrying pLM33 showed higher tolerance to environmental stress conditions, including low acid, high salt, oxidizing, and high-temperature conditions; as well as higher virulence. Furthermore, we found that the plasmid carriage rate of food isolates was 97.22%, with the highest carriage rate of 68.57% for pLM5578, followed by 24.76% for pLM33. Notably, all <em>L. monocytogenes</em> ST5 isolates from ready-to-eat food products (n = 11) carried plasmids, suggesting that contamination of these food products may pose a serious risk to human health. In summary, the results of this study broaden our understanding regarding the role of <em>L. monocytogenes</em> plasmids in stress responses.</div></div>\",\"PeriodicalId\":12399,\"journal\":{\"name\":\"Food microbiology\",\"volume\":\"126 \",\"pages\":\"Article 104675\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0740002024002132\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0740002024002132","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
pLM33 provides tolerance of persistent Listeria monocytogenes ST5 to various stress conditions and also enhances its virulence
Listeria monocytogenes is a major foodborne pathogen. In our previous study, we found that L. monocytogenes ST5 and ST121 strains were dominant in two food plants of Shanghai. Genetic characterization revealed that the environmental tolerance of these strains was attributable to the plasmids pLM33 and pLM5578. To further evaluate the function of L. monocytogenes plasmids, we selected ST5 and ST121 wild-type strains, and used their plasmid-cured strains as controls to conduct tolerance tests. In addition, we analyzed 108 L. monocytogenes strains isolated from four major food categories in Shanghai. Our results showed that compared with the plasmid-cured strain, the ST5 strain carrying pLM33 showed higher tolerance to environmental stress conditions, including low acid, high salt, oxidizing, and high-temperature conditions; as well as higher virulence. Furthermore, we found that the plasmid carriage rate of food isolates was 97.22%, with the highest carriage rate of 68.57% for pLM5578, followed by 24.76% for pLM33. Notably, all L. monocytogenes ST5 isolates from ready-to-eat food products (n = 11) carried plasmids, suggesting that contamination of these food products may pose a serious risk to human health. In summary, the results of this study broaden our understanding regarding the role of L. monocytogenes plasmids in stress responses.
期刊介绍:
Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.