在非急性束缚应激和急性束缚应激条件下,氯尼丁和ACPA对焦虑样行为的调节具有协同作用。

IF 2.7 4区 医学 Q3 NEUROSCIENCES Brain Research Pub Date : 2024-10-29 DOI:10.1016/j.brainres.2024.149304
Amir Chitsaz , Mohaddeseh Ebrahimi-Ghiri , Mohammad-Reza Zarrindast , Fatemeh Khakpai
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引用次数: 0

摘要

本研究探讨了α-2肾上腺素能受体药物(氯尼丁,选择性α-2肾上腺素能受体激动剂;育亨宾,竞争性α-2肾上腺素受体拮抗剂)对非急性束缚应激(NARS)和急性束缚应激(ARS)小鼠体内大麻素CB1受体激动剂花生四烯醇环丙基酰胺(ACPA)效应的可能作用。小鼠单侧左心室植入插管。急性束缚应激是通过4小时的运动束缚进行的。高架迷宫(EPM)装置用于评估焦虑样行为。结果表明,诱导4小时的ARS会导致雄性小鼠的%OAT(张开双臂的时间百分比)降低,从而诱发焦虑样行为。此外,ARS 还会导致前额叶皮层神经元退化。另一方面,单独脑室内注射ACPA(0.5微克/只小鼠)和氯尼丁(0.5微克/只小鼠)会增加OAT%,这表明NARS和ARS小鼠有类似抗焦虑的反应。与此相反,单独静注育亨宾(0.5 µg/只小鼠)会降低OAT%和OAE%(进入开放臂的百分比),这表明对NARS和ARS小鼠有致焦虑样效应。当同时注射阈下剂量的 ACPA 和不同剂量的氯尼替丁时,ACPA 会增强氯尼替丁在 ARS 小鼠中产生的抗焦虑样行为。另一方面,当无效剂量的ACPA和不同剂量的育亨宾同时注射时,ACPA能逆转育亨宾在NARS和ARS小鼠中诱导的致焦虑样效应。此外,研究结果表明,ACPA 和氯尼丁在诱导抗焦虑样行为方面具有协同作用。由此可以得出结论:氯尼丁和 ACPA 之间的相互作用可调节应激对雄性小鼠诱发的焦虑样行为。
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Synergistic interaction between clonidine and ACPA on the modulation of anxiety-like behaviors in non-acute restraint stress and acute restraint stress conditions
The present research examined the possible role of α-2 adrenergic receptor drugs (clonidine, selective α-2 adrenergic receptor agonist, and yohimbine, competitive α-2 adrenoreceptor antagonist,) on the effect of arachidonylcyclopropylamide (ACPA), a cannabinoid CB1 receptor agonist, in non-acute restraint stress (NARS) and acute restraint stress (ARS) mice. The animals were unilaterally implanted with a cannula in the left lateral ventricle. ARS was carried out by movement restraint at a period of 4 h. An elevated plus-maze (EPM) apparatus was used to evaluate anxiety-like behaviors. The results indicated that induction of ARS for 4 h induced anxiogenic-like behavior due to the reduction of %OAT (the percentage of time spent in the open arms) in male mice. Additionally, ARS caused neuronal degeneration in the prefrontal cortex. On the other hand, alone intracerebroventricularly (i.c.v.) infusions of ACPA (0.5 µg/mouse) and clonidine (0.5 µg/mouse) increased %OAT, indicating an anxiolytic-like response in the NARS and ARS mice. In contrast, alone i.c.v. infusions of yohimbine (0.5 µg/mouse) decreased %OAT and %OAE (the percentage of entries to the open arms), proposing an anxiogenic-like effect in the NARS and ARS mice. When the subthreshold dose of ACPA and different doses of clonidine were co-injected, ACPA potentiated the anxiolytic-like behavior produced by clonidine in the ARS mice. On the other hand, when the ineffective dosage of ACPA and different dosages of yohimbine were co-infused, ACPA reversed the anxiogenic-like effect induced by yohimbine in the NARS and ARS mice. Moreover, the results revealed a synergistic effect between ACPA and clonidine upon induction of anxiolytic-like behaviors. It can be concluded that the interaction between clonidine and ACPA modulates the anxiety-like behaviors induced by stress in male mice.
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来源期刊
Brain Research
Brain Research 医学-神经科学
CiteScore
5.90
自引率
3.40%
发文量
268
审稿时长
47 days
期刊介绍: An international multidisciplinary journal devoted to fundamental research in the brain sciences. Brain Research publishes papers reporting interdisciplinary investigations of nervous system structure and function that are of general interest to the international community of neuroscientists. As is evident from the journals name, its scope is broad, ranging from cellular and molecular studies through systems neuroscience, cognition and disease. Invited reviews are also published; suggestions for and inquiries about potential reviews are welcomed. With the appearance of the final issue of the 2011 subscription, Vol. 67/1-2 (24 June 2011), Brain Research Reviews has ceased publication as a distinct journal separate from Brain Research. Review articles accepted for Brain Research are now published in that journal.
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