在 OA 环境中靶向破骨细胞中的 ROS:治疗骨关节炎的新策略

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING Journal of Tissue Engineering Pub Date : 2024-09-24 eCollection Date: 2024-01-01 DOI:10.1177/20417314241279935
Seungho Jeon, Tae Min Kim, Gitae Kwon, Junyoung Park, Sung Young Park, Seoung Hoon Lee, Eun-Jung Jin
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引用次数: 0

摘要

本研究调查了二氧化锰聚合物点(MnO2-PD)包裹的水凝胶(命名为M-PD水凝胶)在调节骨关节炎(OA)环境中活性氧(ROS)方面的治疗潜力。我们的研究强调了水凝胶清除 ROS 的能力,从而影响破骨细胞的分化并保护软骨细胞,为骨关节炎(OA)的治疗提供了一种新方法。我们的研究结果表明,在破骨细胞存在的情况下,M-PD 水凝胶增加了电阻和荧光恢复,这与 ROS 水平降低和破骨细胞分化标记表达受抑制有关。共培养实验揭示了水凝胶通过减少基质降解酶的表达对软骨细胞的保护作用。水凝胶在毛刺孔和/或 OA 诱导的小鼠体内的应用显示,破骨细胞的形成和软骨的破坏显著减少,这表明水凝胶在改变关节微环境方面具有双重治疗作用。这些发现凸显了靶向破骨细胞中的 ROS 作为一种综合治疗方法的潜力,它不仅能缓解症状,还能针对 OA 疾病进展的潜在机制。
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Targeting ROS in osteoclasts within the OA environment: A novel therapeutic strategy for osteoarthritis management.

This study investigated the therapeutic potential of a manganese dioxide-polymer dot (MnO2-PD)-incorporated hydrogel, designated as M-PD hydrogel, for modulating reactive oxygen species (ROS) within the osteoarthritis (OA) environment. Our research highlights the ability of the hydrogel to scavenge ROS, thereby influencing the differentiation of osteoclasts and protecting chondrocytes, offering a novel approach to osteoarthritis (OA) management. Our results indicated that the M-PD hydrogel increased electrical resistance and fluorescence recovery in the presence of osteoclasts, correlating with decreased ROS levels and suppressed expression of osteoclast differentiation markers. Coculture experiments revealed the protective effects of the hydrogel on chondrocytes by reducing the expression of matrix-degrading enzymes. In vivo application in burr holes and/or OA-induced mice revealed a significant reduction in osteoclast formation and cartilage destruction, suggesting the dual therapeutic action of the hydrogel in altering the joint microenvironment. These findings highlight the potential of targeting ROS in osteoclasts as a comprehensive therapeutic approach, offering not only symptomatic relief but also targeting the underlying mechanisms of disease progression in OA.

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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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