从花叶茜根中提取的合成细菌群落可提高离子型稀土矿尾矿中氨氮的去除率。

IF 8.1 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Chemosphere Pub Date : 2024-11-01 DOI:10.1016/j.chemosphere.2024.143650
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引用次数: 0

摘要

硫酸铵作为主要的浸出剂,对稀土元素(REEs)矿区造成了严重的氮污染。植物修复是一种很有前景的修复方法,它依赖于植物与其根部相关微生物群之间的协同关系。然而,利用微生物群加速植物的氮转化和吸收是一项挑战。在这里,我们研究了生长在氨氮(AN)浓度高达 344.35 毫克/千克的稀土尾矿土壤中的先锋植物花叶木(Miscanthus floridulus)根部细菌微生物组的组成、活性和可培养部分。在此基础上,我们构建了一个简化的合成微生物群落(SynCom),该群落来自花叶木的根部,具有硝化和反硝化能力,可帮助稀土尾矿植物有效地将污染物氨氮转化为养分,从而促进植物生长和氨氮去除。该 SynCom 由 10 个细菌菌株组成,包括 Burkholderia 属(5 个)、Paraburkholderia 属(1 个)、Curtobacterium 属(1 个)、Leifsonia 属(1 个)和 Sinomonas 属(2 个)。结果,仅这种 SynCom 就使尾矿土壤中的 AN 含量显著降低了 24.8%。当 SynCom 与植物一起接种时,AN 的降低幅度更大(32.6%),超过了仅靠植物实现的降低幅度(25.5%)。此外,与接种死的 SynCom 相比,接种活的 SynCom 能显著增加芽和根的生物量,分别增加 39.8% 和 49.7%。这些结果表明,AN 的减少可归因于 SynCom 的硝化和反硝化能力,以及它通过刺激植物生长来提高植物氮吸收的能力。值得注意的是,SynCom 的七种硝化和反硝化菌株特别丰富,这表明植物根系会选择性地招募与氮循环相关的细菌,以加速氮的转化和吸收。这些结果为在环境修复工作中利用植物与其根部微生物群之间的协同关系提供了一个实用的解决方案。
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A synthetic bacterial community engineered from Miscanthus floridulus roots enhances ammonia nitrogen removal in ionic rare earth mine tailings
Ammonium sulfate, as the primary leaching agent, has caused significant nitrogen pollution in rare earth elements (REEs) mining areas. Phytoremediation is a promising remediation method, relying on the synergistic relationships between plants and their root-associated microbiome. Nevertheless, harnessing the microbiome to accelerate nitrogen transformation and absorption by plants is challenging. Here, we investigated the composition, activities and culturable fraction of the root bacterial microbiome of the pioneer plant Miscanthus floridulus grown in a REEs tailing soil containing a high ammonia nitrogen (AN) concentration at 344.35 mg kg−1. Based on this, we constructed a simplified synthetic microbial community (SynCom) derived from the roots of M. floridulus, possessing nitrification and denitrification capabilities, to help REEs mine plants efficiently convert pollutant AN into nutrients, thereby enhancing plant growth and AN removal. This SynCom, consisting of 10 bacterial strains, included species of the genera Burkholderia (5) Paraburkholderia (1), Curtobacterium (1), Leifsonia (1) and Sinomonas (2). As a result, this SynCom alone achieved a significant reduction of 24.8% in AN content in tailing soil. When the SynCom inoculated with plants, the reduction in AN was even more significant (32.6%), surpassing the reduction achieved solely by plants (25.5%). Moreover, live SynCom inoculation significantly increased shoot and root biomass by 39.8% and 49.7%, respectively, compared to dead SynCom inoculation. These results indicate that the reduction in AN can be attributed to the SynCom's nitrification and denitrification capabilities, as well as its ability to enhance plant nitrogen absorption by stimulating their growth. Notably, seven nitrifying and denitrifying strains of the SynCom are particularly enriched, suggesting that plant roots selectively recruit nitrogen cycle-related bacteria to accelerate nitrogen transformation and absorption. These results provide a practical solution for harnessing the synergistic relationships between plants and their root microbiome in environmental remediation efforts.
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来源期刊
Chemosphere
Chemosphere 环境科学-环境科学
CiteScore
15.80
自引率
8.00%
发文量
4975
审稿时长
3.4 months
期刊介绍: Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.
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