具有多基因活性的下一代 RNA/Protein Carrying Vector。

IF 9 2区 医学 Q1 VIROLOGY Reviews in Medical Virology Pub Date : 2024-11-01 DOI:10.1002/rmv.70008
Tetsuya Nosaka, Junpei Ohtsuka, Tomomi Ohtsuka, Masayuki Fukumura
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引用次数: 0

摘要

人副流感病毒 2 型(hPIV2)是婴幼儿普通感冒的病原体之一,它是一种非片段负义 RNA 病毒,具有强大的基因表达系统。它在人的一生中反复感染,但不会引起严重疾病。由于 hPIV2 的病毒包膜可携带异位蛋白,我们通过删除 hPIV2 中的 F 基因,开发出了一种非繁殖型 RNA/ 蛋白携带载体 BC-PIV。BC-PIV能在表达hPIV2 F基因的稳定包装细胞系Vero/BC-F细胞中旺盛增殖,但不能在其他细胞中增殖。BC-PIV 能以插入基因片段的形式在多基因 RNA 基因组上传递外源基因,同时以膜锚定形式在其包膜上传递外源蛋白质。例如,流感病毒 M2e 蛋白、埃博拉病毒 GP 蛋白和严重急性呼吸系统综合征-冠状病毒-2(SARS-CoV-2)尖峰蛋白在包装细胞中高度表达,并与病毒结合。埃博拉病毒 GP 蛋白和 SARS-CoV-2 尖峰蛋白分别通过 BC-PIV 传播,即使事先分别用重组 BC-PIV 处理小鼠和仓鼠,也能有效诱导针对每种病毒的中和抗体。在这篇综述中,我们描述了 BC-PIV 作为一种有前途的疫苗载体的特性,并展示了它作为抗肿瘤病毒的应用。
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Next Generation RNA/Protein-Carrying Vector With Pleiotropic Activity.

Human parainfluenza virus type 2 (hPIV2), one of the causative agents of infantile common cold, is a non-segmented negative-sense RNA virus with a robust gene expression system. It infects recurrently throughout human life without causing severe disease. Because hPIV2 has a viral envelope that can carry ectopic proteins, we developed a non-propagative RNA/protein-carrying vector BC-PIV by deleting the F gene from hPIV2. BC-PIV can be vigorously proliferated in the stable packaging cell line Vero/BC-F cells expressing the hPIV2 F gene but not in other cells. BC-PIV can deliver exogenous gene(s) on a multigenic RNA genome as an inserted gene fragment(s) and simultaneously deliver exogenous protein(s) on its envelope in a membrane-anchored form. For example, influenza virus M2e protein, Ebola virus GP protein, and severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) spike protein were shown to be highly expressed in packaging cells and incorporated into the virion. The Ebola virus GP protein and SARS-CoV-2 spike protein, each delivered via BC-PIV, efficiently induced neutralising antibodies against each virus, even after prior treatment with recombinant BC-PIV in mice and hamsters, respectively. In this review, we describe the properties of BC-PIV as a promising vaccine vector, and also demonstrate its application as an anti-tumour virus.

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来源期刊
Reviews in Medical Virology
Reviews in Medical Virology 医学-病毒学
CiteScore
21.40
自引率
0.90%
发文量
88
期刊介绍: Reviews in Medical Virology aims to provide articles reviewing conceptual or technological advances in diverse areas of virology. The journal covers topics such as molecular biology, cell biology, replication, pathogenesis, immunology, immunization, epidemiology, diagnosis, treatment of viruses of medical importance, and COVID-19 research. The journal has an Impact Factor of 6.989 for the year 2020. The readership of the journal includes clinicians, virologists, medical microbiologists, molecular biologists, infectious disease specialists, and immunologists. Reviews in Medical Virology is indexed and abstracted in databases such as CABI, Abstracts in Anthropology, ProQuest, Embase, MEDLINE/PubMed, ProQuest Central K-494, SCOPUS, and Web of Science et,al.
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