超声辅助深共晶溶剂萃取的优化、表征以及植物精液多糖的生物活性。

IF 2 4区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS Preparative Biochemistry & Biotechnology Pub Date : 2024-11-04 DOI:10.1080/10826068.2024.2423640
Yao Wen, Geping Chen, Lei Hu
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引用次数: 0

摘要

车前子精(PS)是车前子科植物车前子(Plantago asiatica L. 或 Plantagodepressa Willd.)的干燥成熟种子。其多糖是 PS 的重要成分。采用响应面方法对超声波辅助深共晶溶剂(DES)萃取 PS 多糖(PSP)的工艺进行了优化。结果表明,最佳萃取参数为固液比 1:35 g/mL、萃取时间 73 min、摩尔比 2:1。水浸法和超声波水提取法的PSP收率分别为0.64%和1.20%,表明DES提取法(2.21±0.06%)优于上述两种方法,优化效果良好。通过α-葡萄糖苷酶和α-淀粉酶抑制活性实验,发现PSPs-1的IC50值分别为1122和220.5 μg/mL。DPPH 和 ABTS+ 清除活性实验表明,PSPs-1 的 IC50 值分别为 19.2 和 4.3 μg/mL。其单糖组成摩尔比为鼠李糖:半乳糖:半乳糖醛酸:葡萄糖:葡萄糖醛酸:阿拉伯糖:甘露糖:木糖=33.6:13.3:6.5:3:2.6:2:1.4:1。因此,这项研究可为多糖工业化生产工艺的建立及其生物活性的研究提供实验依据。
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Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from Plantaginis Semen.

Plantaginis Semen (PS) are the dried mature seeds of Plantago asiatica L. or Plantagodepressa Willd. in the Plantago family. Its polysaccharides are important components of PS. Response surface methodology was used to optimize the ultrasonic-assisted deep eutectic solvent (DES) extraction process of PS polysaccharides (PSP). The results showed that the optimal extraction parameters were a solid-liquid ratio of 1:35 g/mL, an extraction time of 73 min, and a molar ratio of 2:1. The yield of PSP was 0.64% and 1.20% by water immersion and ultrasonic water extraction, respectively, indicating that the DES extraction method (2.21 ± 0.06%) is superior to these two methods, and the optimization effect is good. Through the α-glucosidase and α-amylase inhibition activities experiment, it was found that the IC50 values of PSPs-1 were 1122 and 220.5 μg/mL. DPPH·and ABTS+ scavenging activity experiments showed that the IC50 values of PSPs-1 were 19.2 and 4.3 μg/mL, respectively. Its molar ratio of monosaccharide composition is rhamnose: galactose: galacturonic acid: glucose: glucuronic acid: arabinose: mannose: xylose = 33.6:13.3:6.5:3:2.6:2:1.4:1. Therefore, this study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.

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来源期刊
Preparative Biochemistry & Biotechnology
Preparative Biochemistry & Biotechnology 工程技术-生化研究方法
CiteScore
4.90
自引率
3.40%
发文量
98
审稿时长
2 months
期刊介绍: Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.
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