超声辅助深共晶溶剂萃取的优化、表征以及植物精液多糖的生物活性。

IF 2 4区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS Preparative Biochemistry & Biotechnology Pub Date : 2024-11-04 DOI:10.1080/10826068.2024.2423640
Yao Wen, Geping Chen, Lei Hu
{"title":"超声辅助深共晶溶剂萃取的优化、表征以及植物精液多糖的生物活性。","authors":"Yao Wen, Geping Chen, Lei Hu","doi":"10.1080/10826068.2024.2423640","DOIUrl":null,"url":null,"abstract":"<p><p><i>Plantaginis Semen</i> (PS) are the dried mature seeds of <i>Plantago asiatica</i> L. or <i>Plantagodepressa</i> Willd. in the Plantago family. Its polysaccharides are important components of <i>PS</i>. Response surface methodology was used to optimize the ultrasonic-assisted deep eutectic solvent (DES) extraction process of PS polysaccharides (PSP). The results showed that the optimal extraction parameters were a solid-liquid ratio of 1:35 g/mL, an extraction time of 73 min, and a molar ratio of 2:1. The yield of PSP was 0.64% and 1.20% by water immersion and ultrasonic water extraction, respectively, indicating that the DES extraction method (2.21 ± 0.06%) is superior to these two methods, and the optimization effect is good. Through the α-glucosidase and α-amylase inhibition activities experiment, it was found that the IC<sub>50</sub> values of PSPs-1 were 1122 and 220.5 μg/mL. DPPH·and ABTS<sup>+</sup> scavenging activity experiments showed that the IC<sub>50</sub> values of PSPs-1 were 19.2 and 4.3 μg/mL, respectively. Its molar ratio of monosaccharide composition is rhamnose: galactose: galacturonic acid: glucose: glucuronic acid: arabinose: mannose: xylose = 33.6:13.3:6.5:3:2.6:2:1.4:1. Therefore, this study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from <i>Plantaginis Semen</i>.\",\"authors\":\"Yao Wen, Geping Chen, Lei Hu\",\"doi\":\"10.1080/10826068.2024.2423640\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Plantaginis Semen</i> (PS) are the dried mature seeds of <i>Plantago asiatica</i> L. or <i>Plantagodepressa</i> Willd. in the Plantago family. Its polysaccharides are important components of <i>PS</i>. Response surface methodology was used to optimize the ultrasonic-assisted deep eutectic solvent (DES) extraction process of PS polysaccharides (PSP). The results showed that the optimal extraction parameters were a solid-liquid ratio of 1:35 g/mL, an extraction time of 73 min, and a molar ratio of 2:1. The yield of PSP was 0.64% and 1.20% by water immersion and ultrasonic water extraction, respectively, indicating that the DES extraction method (2.21 ± 0.06%) is superior to these two methods, and the optimization effect is good. Through the α-glucosidase and α-amylase inhibition activities experiment, it was found that the IC<sub>50</sub> values of PSPs-1 were 1122 and 220.5 μg/mL. DPPH·and ABTS<sup>+</sup> scavenging activity experiments showed that the IC<sub>50</sub> values of PSPs-1 were 19.2 and 4.3 μg/mL, respectively. Its molar ratio of monosaccharide composition is rhamnose: galactose: galacturonic acid: glucose: glucuronic acid: arabinose: mannose: xylose = 33.6:13.3:6.5:3:2.6:2:1.4:1. Therefore, this study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.</p>\",\"PeriodicalId\":20401,\"journal\":{\"name\":\"Preparative Biochemistry & Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-11-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Preparative Biochemistry & Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1080/10826068.2024.2423640\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Preparative Biochemistry & Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/10826068.2024.2423640","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

摘要

车前子精(PS)是车前子科植物车前子(Plantago asiatica L. 或 Plantagodepressa Willd.)的干燥成熟种子。其多糖是 PS 的重要成分。采用响应面方法对超声波辅助深共晶溶剂(DES)萃取 PS 多糖(PSP)的工艺进行了优化。结果表明,最佳萃取参数为固液比 1:35 g/mL、萃取时间 73 min、摩尔比 2:1。水浸法和超声波水提取法的PSP收率分别为0.64%和1.20%,表明DES提取法(2.21±0.06%)优于上述两种方法,优化效果良好。通过α-葡萄糖苷酶和α-淀粉酶抑制活性实验,发现PSPs-1的IC50值分别为1122和220.5 μg/mL。DPPH 和 ABTS+ 清除活性实验表明,PSPs-1 的 IC50 值分别为 19.2 和 4.3 μg/mL。其单糖组成摩尔比为鼠李糖:半乳糖:半乳糖醛酸:葡萄糖:葡萄糖醛酸:阿拉伯糖:甘露糖:木糖=33.6:13.3:6.5:3:2.6:2:1.4:1。因此,这项研究可为多糖工业化生产工艺的建立及其生物活性的研究提供实验依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from Plantaginis Semen.

Plantaginis Semen (PS) are the dried mature seeds of Plantago asiatica L. or Plantagodepressa Willd. in the Plantago family. Its polysaccharides are important components of PS. Response surface methodology was used to optimize the ultrasonic-assisted deep eutectic solvent (DES) extraction process of PS polysaccharides (PSP). The results showed that the optimal extraction parameters were a solid-liquid ratio of 1:35 g/mL, an extraction time of 73 min, and a molar ratio of 2:1. The yield of PSP was 0.64% and 1.20% by water immersion and ultrasonic water extraction, respectively, indicating that the DES extraction method (2.21 ± 0.06%) is superior to these two methods, and the optimization effect is good. Through the α-glucosidase and α-amylase inhibition activities experiment, it was found that the IC50 values of PSPs-1 were 1122 and 220.5 μg/mL. DPPH·and ABTS+ scavenging activity experiments showed that the IC50 values of PSPs-1 were 19.2 and 4.3 μg/mL, respectively. Its molar ratio of monosaccharide composition is rhamnose: galactose: galacturonic acid: glucose: glucuronic acid: arabinose: mannose: xylose = 33.6:13.3:6.5:3:2.6:2:1.4:1. Therefore, this study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Preparative Biochemistry & Biotechnology
Preparative Biochemistry & Biotechnology 工程技术-生化研究方法
CiteScore
4.90
自引率
3.40%
发文量
98
审稿时长
2 months
期刊介绍: Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.
期刊最新文献
Engineered production of 5-aminolevulinic acid in recombinant Escherichia coli BL21. Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from Plantaginis Semen. A comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures. Kinetic and thermodynamic investigation of Rhodanese synthesized by enhanced Klebsiella oxytoca JCM 1665 strain: a comparative between the free and immobilized enzyme entrapped in alginate beads. Statistical optimization and sequential scale-up of α-galactosidase production by Actinoplanes utahensis B1 from shake flask to pilot scale.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1