膳食胆汁酸可缓解皮质酮诱导的肉鸡脂肪肝和肝糖皮质激素受体抑制。

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of animal science Pub Date : 2024-11-04 DOI:10.1093/jas/skae338
Jie Liu, Ke Zhang, Mindie Zhao, Liang Chen, Huimin Chen, Yulan Zhao, Ruqian Zhao
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引用次数: 0

摘要

本研究旨在探讨胆汁酸(BA)对皮质酮诱发肉鸡脂肪肝的缓解作用及其机制。将雄性 Arbor Acres 鸡随机分为三组:对照组(CON)、应激模型组(CORT)和 BA 处理组(CORT-BA)。CORT-BA 组从 21 日龄开始饲喂含 250 毫克/千克 BA 的饲料。从第36天到第43天,CORT组和CORT-BA组都接受皮质酮皮下注射以模拟慢性应激。结果表明,BA能明显减轻皮质酮导致的体重下降、肝脏肿大和肝脏脂质沉积(P < 0.05)。肝脏RNA-seq分析表明,BA通过抑制脂质代谢途径,包括脂肪酸的生物合成、甘油三酯的生物合成和脂肪酸的转运,缓解了皮质酮诱导的脂肪肝。此外,BA 还能改善皮质酮诱导的糖皮质激素受体(GR)表达下调(P < 0.05)。分子对接和细胞热转移试验显示,复方胆汁酸的主要成分--透明脱氧胆酸(HDCA)可与 GR 结合并增强其稳定性。总之,BA通过抑制脂质合成途径和减轻对肝脏GR表达的抑制,缓解了皮质酮诱导的肉鸡脂肪肝。
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Dietary bile acids alleviate corticosterone-induced fatty liver and hepatic glucocorticoid receptor suppression in broiler chickens.

The aim of this study was to investigate the alleviating effects and mechanisms of bile acids (BA) on corticosterone-induced fatty liver in broiler chickens. Male Arbor Acres chickens were randomly divided into three groups: control group (CON), stress model group (CORT), and BA-treated group (CORT-BA). The CORT-BA group received a diet with 250 mg/kg BA from 21 days of age. From day 36 to 43, both the CORT and CORT-BA groups received subcutaneous injections of corticosterone to simulate chronic stress. The results indicated that BA significantly mitigated the body weight loss, liver enlargement, and hepatic lipid deposition caused by corticosterone (P < 0.05). Liver RNA-seq analysis showed that BA alleviated corticosterone-induced fatty liver by inhibiting lipid metabolism pathways, including fatty acid biosynthesis, triglyceride biosynthesis, and fatty acid transport. Additionally, BA improved corticosterone-induced downregulation of glucocorticoid receptor (GR) expression (P < 0.05). Molecular docking and cellular thermal shift assays revealed that hyodeoxycholic acid (HDCA), a major component of compound bile acids, could bind to GR and enhance its stability. In conclusion, BA alleviated corticosterone-induced fatty liver in broilers by inhibiting lipid synthesis pathways and mitigating the suppression of hepatic GR expression.

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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
期刊最新文献
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