Majid Almasi, Golnaz Shafiei, Hossein Nikzad, Mohammad Karimian, Ghazaleh Moshkdanian
{"title":"左旋肉碱对环磷酰胺治疗后小鼠活性氧减少和凋亡基因表达的影响:一项实验研究。","authors":"Majid Almasi, Golnaz Shafiei, Hossein Nikzad, Mohammad Karimian, Ghazaleh Moshkdanian","doi":"10.18502/ijrm.v22i8.17262","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Cyclophosphamide (CP), a utilized anticancer drug, is known to cause infertility in women. However, L-carnitine (LC), an antioxidant, has been shown to offer protective benefits against infertility.</p><p><strong>Objective: </strong>This study aimed to evaluate the levels of reactive oxygen species (ROS) and apoptotic gene expression in mice treated with CP and LC.</p><p><strong>Materials and methods: </strong>24 NMRI female mice (6-8 wk, 30 <math><mo>±</mo></math> 5 gr) were divided into 4 groups: control group: received normal saline intraperitoneal (IP) injection for 10 days; CP group: received 75 mg/kg of CP as a single IP on the 10 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> day of the experiment; LC group: received 200 mg/kg of LC IP for 10 days; LC+CP group: received LC for 10 days and CP single IP injection on the 10 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> day of the experiment. After 10 days, mice were superovulated. The oviducts were then removed, and the oocytes of each group were collected for evaluating apoptotic gene expression B-cell lymphoma 2(<i>Bcl2</i>), <i>Bcl2</i>-associated X(<i>Bax</i>), and <i>Caspase3</i> via real-time polymerase chain reaction and intracellular ROS levels by dichloro-dihydro-fluorescein diacetate fluorescence staining.</p><p><strong>Results: </strong>Data revealed that LC in the LC+CP group significantly increased <i>Bcl2</i> gene expression (p = 0.01), and decreased <i>Bax</i> and <i>Caspase3</i> gene expression compared to the CP group (p = 0.03, p = 0.04). LC decreased the ROS level in the LC+CP group compared to the CP group (p <math><mo><</mo></math> 0.001).</p><p><strong>Conclusion: </strong>Findings suggest that LC can scavenge the ROS caused by CP and modulate the apoptotic pathway via downregulating the <i>Bax</i> and <i>Caspase3</i> genes and upregulating the <i>Bcl2</i> gene in oocytes of mice exposed to CP.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528291/pdf/","citationCount":"0","resultStr":"{\"title\":\"The effect of L-carnitine in reactive oxygen species reduction and apoptotic gene expression in mice after cyclophosphamide: An experimental study.\",\"authors\":\"Majid Almasi, Golnaz Shafiei, Hossein Nikzad, Mohammad Karimian, Ghazaleh Moshkdanian\",\"doi\":\"10.18502/ijrm.v22i8.17262\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Cyclophosphamide (CP), a utilized anticancer drug, is known to cause infertility in women. However, L-carnitine (LC), an antioxidant, has been shown to offer protective benefits against infertility.</p><p><strong>Objective: </strong>This study aimed to evaluate the levels of reactive oxygen species (ROS) and apoptotic gene expression in mice treated with CP and LC.</p><p><strong>Materials and methods: </strong>24 NMRI female mice (6-8 wk, 30 <math><mo>±</mo></math> 5 gr) were divided into 4 groups: control group: received normal saline intraperitoneal (IP) injection for 10 days; CP group: received 75 mg/kg of CP as a single IP on the 10 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> day of the experiment; LC group: received 200 mg/kg of LC IP for 10 days; LC+CP group: received LC for 10 days and CP single IP injection on the 10 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> day of the experiment. After 10 days, mice were superovulated. The oviducts were then removed, and the oocytes of each group were collected for evaluating apoptotic gene expression B-cell lymphoma 2(<i>Bcl2</i>), <i>Bcl2</i>-associated X(<i>Bax</i>), and <i>Caspase3</i> via real-time polymerase chain reaction and intracellular ROS levels by dichloro-dihydro-fluorescein diacetate fluorescence staining.</p><p><strong>Results: </strong>Data revealed that LC in the LC+CP group significantly increased <i>Bcl2</i> gene expression (p = 0.01), and decreased <i>Bax</i> and <i>Caspase3</i> gene expression compared to the CP group (p = 0.03, p = 0.04). LC decreased the ROS level in the LC+CP group compared to the CP group (p <math><mo><</mo></math> 0.001).</p><p><strong>Conclusion: </strong>Findings suggest that LC can scavenge the ROS caused by CP and modulate the apoptotic pathway via downregulating the <i>Bax</i> and <i>Caspase3</i> genes and upregulating the <i>Bcl2</i> gene in oocytes of mice exposed to CP.</p>\",\"PeriodicalId\":14386,\"journal\":{\"name\":\"International Journal of Reproductive Biomedicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528291/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Reproductive Biomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijrm.v22i8.17262\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Reproductive Biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijrm.v22i8.17262","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
The effect of L-carnitine in reactive oxygen species reduction and apoptotic gene expression in mice after cyclophosphamide: An experimental study.
Background: Cyclophosphamide (CP), a utilized anticancer drug, is known to cause infertility in women. However, L-carnitine (LC), an antioxidant, has been shown to offer protective benefits against infertility.
Objective: This study aimed to evaluate the levels of reactive oxygen species (ROS) and apoptotic gene expression in mice treated with CP and LC.
Materials and methods: 24 NMRI female mice (6-8 wk, 30 5 gr) were divided into 4 groups: control group: received normal saline intraperitoneal (IP) injection for 10 days; CP group: received 75 mg/kg of CP as a single IP on the 10 day of the experiment; LC group: received 200 mg/kg of LC IP for 10 days; LC+CP group: received LC for 10 days and CP single IP injection on the 10 day of the experiment. After 10 days, mice were superovulated. The oviducts were then removed, and the oocytes of each group were collected for evaluating apoptotic gene expression B-cell lymphoma 2(Bcl2), Bcl2-associated X(Bax), and Caspase3 via real-time polymerase chain reaction and intracellular ROS levels by dichloro-dihydro-fluorescein diacetate fluorescence staining.
Results: Data revealed that LC in the LC+CP group significantly increased Bcl2 gene expression (p = 0.01), and decreased Bax and Caspase3 gene expression compared to the CP group (p = 0.03, p = 0.04). LC decreased the ROS level in the LC+CP group compared to the CP group (p 0.001).
Conclusion: Findings suggest that LC can scavenge the ROS caused by CP and modulate the apoptotic pathway via downregulating the Bax and Caspase3 genes and upregulating the Bcl2 gene in oocytes of mice exposed to CP.
期刊介绍:
The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.