左旋肉碱对环磷酰胺治疗后小鼠活性氧减少和凋亡基因表达的影响:一项实验研究。

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY International Journal of Reproductive Biomedicine Pub Date : 2024-10-14 eCollection Date: 2024-08-01 DOI:10.18502/ijrm.v22i8.17262
Majid Almasi, Golnaz Shafiei, Hossein Nikzad, Mohammad Karimian, Ghazaleh Moshkdanian
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引用次数: 0

摘要

背景:环磷酰胺(CP)是一种常用的抗癌药物,已知会导致妇女不孕。然而,左旋肉碱(LC)作为一种抗氧化剂,已被证明对不孕症有保护作用:本研究旨在评估使用 CP 和 LC 治疗的小鼠体内活性氧(ROS)水平和凋亡基因表达。材料和方法:将 24 只 NMRI 雌性小鼠(6-8 wk,30 ± 5 gr)分为 4 组:对照组:接受正常生理盐水腹腔注射(IP),为期 10 天;CP 组:在实验的第 10 天接受 75 mg/kg CP 单次 IP 注射;LC 组:在实验的第 10 天接受 200 mg/kg LC IP 注射;LC+CP 组:在实验的第 10 天接受 LC 和 CP 单次 IP 注射,为期 10 天。10 天后,小鼠超排卵。然后取出输卵管,收集各组的卵母细胞,通过实时聚合酶链式反应评估B细胞淋巴瘤2(Bcl2)、Bcl2相关X(Bax)和Caspase3的凋亡基因表达,并通过二氯二氢荧光素二乙酸酯荧光染色评估细胞内ROS水平:数据显示,与CP组相比,LC+CP组的LC明显增加了Bcl2基因的表达(p = 0.01),降低了Bax和Caspase3基因的表达(p = 0.03,p = 0.04)。与 CP 组相比,LC+CP 组的 ROS 水平降低(p 0.001):结论:研究结果表明,LC 可以清除由 CP 引起的 ROS,并通过下调 Bax 和 Caspase3 基因以及上调 Bcl2 基因来调节暴露于 CP 的小鼠卵母细胞的凋亡途径。
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The effect of L-carnitine in reactive oxygen species reduction and apoptotic gene expression in mice after cyclophosphamide: An experimental study.

Background: Cyclophosphamide (CP), a utilized anticancer drug, is known to cause infertility in women. However, L-carnitine (LC), an antioxidant, has been shown to offer protective benefits against infertility.

Objective: This study aimed to evaluate the levels of reactive oxygen species (ROS) and apoptotic gene expression in mice treated with CP and LC.

Materials and methods: 24 NMRI female mice (6-8 wk, 30 ± 5 gr) were divided into 4 groups: control group: received normal saline intraperitoneal (IP) injection for 10 days; CP group: received 75 mg/kg of CP as a single IP on the 10 th day of the experiment; LC group: received 200 mg/kg of LC IP for 10 days; LC+CP group: received LC for 10 days and CP single IP injection on the 10 th day of the experiment. After 10 days, mice were superovulated. The oviducts were then removed, and the oocytes of each group were collected for evaluating apoptotic gene expression B-cell lymphoma 2(Bcl2), Bcl2-associated X(Bax), and Caspase3 via real-time polymerase chain reaction and intracellular ROS levels by dichloro-dihydro-fluorescein diacetate fluorescence staining.

Results: Data revealed that LC in the LC+CP group significantly increased Bcl2 gene expression (p = 0.01), and decreased Bax and Caspase3 gene expression compared to the CP group (p = 0.03, p = 0.04). LC decreased the ROS level in the LC+CP group compared to the CP group (p < 0.001).

Conclusion: Findings suggest that LC can scavenge the ROS caused by CP and modulate the apoptotic pathway via downregulating the Bax and Caspase3 genes and upregulating the Bcl2 gene in oocytes of mice exposed to CP.

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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
93
审稿时长
16 weeks
期刊介绍: The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
期刊最新文献
Aspirin before the 11 th week of pregnancy to prevent pre-eclampsia. Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study. Fixed versus flexible gonadotropin releasing hormone antagonist protocol in women with polycystic ovary syndrome undergoing in vitro fertilization: An RCT. The association among cancer-antigen-125 and pre-eclampsia and estimated fetal weight: A case-control study. The effect of L-carnitine in reactive oxygen species reduction and apoptotic gene expression in mice after cyclophosphamide: An experimental study.
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