{"title":"vhvp-2基因在引起半透明后幼虫病(TPD)的弧菌中的分布特点和多样性。","authors":"","doi":"10.1016/j.jip.2024.108228","DOIUrl":null,"url":null,"abstract":"<div><div>Translucent post-larva vibriosis (<em>V</em><sub>TPD</sub>) in <em>Penaeus vannamei</em>, caused by the virulence factor <em>Vibrio</em> high virulent protein 2 (<em>vhvp-2</em>) gene, poses a significant threat to the shrimp aquaculture industry in China. This study conducted a comprehensive analysis of the <em>vhvp-2</em> gene distribution and genetic diversity among <em>Vibrio</em>, which play a key role in the pathogenesis of translucent post-larvae disease (TPD). A total of 6281 <em>Vibrio</em> genomes from the NCBI database were screened, revealing the presence of the <em>vhvp-2</em> gene in 26 strains, predominantly in <em>V. parahaemolyticus</em>, with diverse locations on both plasmids and chromosomes. Comparative analysis of the genomes, plasmids, and the <em>vhvp-2</em> gene successfully differentiated three distinct clusters of <em>Vibrio</em> species and identified at least two major plasmid groups. However, these plasmid groups did not exhibit the same evolutionary relationships as the host bacteria themselves. The analysis also revealed the conserved presence of the <em>vhvp-2</em> gene along with genetic variations. Moreover, the widespread presence of antimicrobial resistance genes (ARGs) and virulence factors in <em>Vibrio</em> strains enhances their pathogenicity and poses increased public health risks. The findings underscore the importance of understanding the adaptability, evolution, and ecological characteristics of <em>Vibrio</em> strains harboring <em>vhvp-2</em> gene. This research provides a scientific foundation for the development of effective disease prevention and control strategies, contributing to the mitigation of TPD’s impact on the shrimp aquaculture industry and supporting its sustainable and healthy growth.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characteristics of vhvp-2 gene distribution and diversity within the Vibrio causing translucent post-larvae disease (TPD)\",\"authors\":\"\",\"doi\":\"10.1016/j.jip.2024.108228\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Translucent post-larva vibriosis (<em>V</em><sub>TPD</sub>) in <em>Penaeus vannamei</em>, caused by the virulence factor <em>Vibrio</em> high virulent protein 2 (<em>vhvp-2</em>) gene, poses a significant threat to the shrimp aquaculture industry in China. This study conducted a comprehensive analysis of the <em>vhvp-2</em> gene distribution and genetic diversity among <em>Vibrio</em>, which play a key role in the pathogenesis of translucent post-larvae disease (TPD). A total of 6281 <em>Vibrio</em> genomes from the NCBI database were screened, revealing the presence of the <em>vhvp-2</em> gene in 26 strains, predominantly in <em>V. parahaemolyticus</em>, with diverse locations on both plasmids and chromosomes. Comparative analysis of the genomes, plasmids, and the <em>vhvp-2</em> gene successfully differentiated three distinct clusters of <em>Vibrio</em> species and identified at least two major plasmid groups. However, these plasmid groups did not exhibit the same evolutionary relationships as the host bacteria themselves. The analysis also revealed the conserved presence of the <em>vhvp-2</em> gene along with genetic variations. Moreover, the widespread presence of antimicrobial resistance genes (ARGs) and virulence factors in <em>Vibrio</em> strains enhances their pathogenicity and poses increased public health risks. The findings underscore the importance of understanding the adaptability, evolution, and ecological characteristics of <em>Vibrio</em> strains harboring <em>vhvp-2</em> gene. This research provides a scientific foundation for the development of effective disease prevention and control strategies, contributing to the mitigation of TPD’s impact on the shrimp aquaculture industry and supporting its sustainable and healthy growth.</div></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S002220112400171X\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S002220112400171X","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
Characteristics of vhvp-2 gene distribution and diversity within the Vibrio causing translucent post-larvae disease (TPD)
Translucent post-larva vibriosis (VTPD) in Penaeus vannamei, caused by the virulence factor Vibrio high virulent protein 2 (vhvp-2) gene, poses a significant threat to the shrimp aquaculture industry in China. This study conducted a comprehensive analysis of the vhvp-2 gene distribution and genetic diversity among Vibrio, which play a key role in the pathogenesis of translucent post-larvae disease (TPD). A total of 6281 Vibrio genomes from the NCBI database were screened, revealing the presence of the vhvp-2 gene in 26 strains, predominantly in V. parahaemolyticus, with diverse locations on both plasmids and chromosomes. Comparative analysis of the genomes, plasmids, and the vhvp-2 gene successfully differentiated three distinct clusters of Vibrio species and identified at least two major plasmid groups. However, these plasmid groups did not exhibit the same evolutionary relationships as the host bacteria themselves. The analysis also revealed the conserved presence of the vhvp-2 gene along with genetic variations. Moreover, the widespread presence of antimicrobial resistance genes (ARGs) and virulence factors in Vibrio strains enhances their pathogenicity and poses increased public health risks. The findings underscore the importance of understanding the adaptability, evolution, and ecological characteristics of Vibrio strains harboring vhvp-2 gene. This research provides a scientific foundation for the development of effective disease prevention and control strategies, contributing to the mitigation of TPD’s impact on the shrimp aquaculture industry and supporting its sustainable and healthy growth.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.