{"title":"从消化酶的角度看暴露于 GO 纳米粒子的家养蟋蟀膳食中脆弱的本体发育阶段--成年幼体。","authors":"","doi":"10.1016/j.chemosphere.2024.143641","DOIUrl":null,"url":null,"abstract":"<div><div>Graphene oxide nanoparticles (GO) (have promising properties for, electronics, energy, medicine, water purification, agriculture and food production industry. However, their potentially hazardous effects are still not satisfactorily recognized, so they are often included in the group of contaminants of emerging concern. Therefore, the aim of this investigation was to assess the potentially harmful effects of orally administered GO on the digestive enzyme activities of the house crickets <em>Acheta domesticus</em>. The activity of digestive enzymes was measured using the API®ZYM test and the results were compared in relation to the insect age group (Larvae, Adult Young and Adult Mature) and previous selection towards longevity (‘long living’ <em>vs</em> ‘wild’ strains). General analysis, encompassing all digestive enzymes simultaneously, as well as an analysis performed for individual enzyme subgroups, revealed that both factors and their interactions significantly modified the activity of digestive enzymes of <em>A. domesticus</em>. The most pronounced effect was connected with the age factor. The study revealed that the Adult Young age group was the most sensitive; in this group, the activity of digestive enzymes was, in general, decreased in comparison with the control group, while the remaining group did not respond to GO with a similar intensity. In turn, the inter-strain comparisons demonstrated different response patterns to the GO. The control enzymatic activity of the insects from long living’ strain was, in general, higher than in the ‘wild strain’; however, it was significantly decreased in the GO-exposed groups, while in the ‘wild’ strain, the activity remained relatively homogenous. Additionally, the tendency was more pronounced in Adult Young than in the remaining age groups. The potential toxicity of GO requires further investigation, mainly with a focus on the composition and functioning of gut microbiotas.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":null,"pages":null},"PeriodicalIF":8.1000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Adult young as the fragile ontogenetic stage of the house crickets dietary exposed to GO nanoparticles – digestive enzymes perspective\",\"authors\":\"\",\"doi\":\"10.1016/j.chemosphere.2024.143641\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Graphene oxide nanoparticles (GO) (have promising properties for, electronics, energy, medicine, water purification, agriculture and food production industry. However, their potentially hazardous effects are still not satisfactorily recognized, so they are often included in the group of contaminants of emerging concern. Therefore, the aim of this investigation was to assess the potentially harmful effects of orally administered GO on the digestive enzyme activities of the house crickets <em>Acheta domesticus</em>. The activity of digestive enzymes was measured using the API®ZYM test and the results were compared in relation to the insect age group (Larvae, Adult Young and Adult Mature) and previous selection towards longevity (‘long living’ <em>vs</em> ‘wild’ strains). General analysis, encompassing all digestive enzymes simultaneously, as well as an analysis performed for individual enzyme subgroups, revealed that both factors and their interactions significantly modified the activity of digestive enzymes of <em>A. domesticus</em>. The most pronounced effect was connected with the age factor. The study revealed that the Adult Young age group was the most sensitive; in this group, the activity of digestive enzymes was, in general, decreased in comparison with the control group, while the remaining group did not respond to GO with a similar intensity. In turn, the inter-strain comparisons demonstrated different response patterns to the GO. The control enzymatic activity of the insects from long living’ strain was, in general, higher than in the ‘wild strain’; however, it was significantly decreased in the GO-exposed groups, while in the ‘wild’ strain, the activity remained relatively homogenous. Additionally, the tendency was more pronounced in Adult Young than in the remaining age groups. The potential toxicity of GO requires further investigation, mainly with a focus on the composition and functioning of gut microbiotas.</div></div>\",\"PeriodicalId\":276,\"journal\":{\"name\":\"Chemosphere\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":8.1000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemosphere\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0045653524025414\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemosphere","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0045653524025414","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
氧化石墨烯纳米粒子(GO)在电子、能源、医药、水净化、农业和食品生产等领域具有广阔的应用前景。然而,其潜在的有害影响仍未得到令人满意的认识,因此经常被列入新出现的污染物类别。因此,本研究旨在评估口服 GO 对家养蟋蟀(Acheta domesticus)消化酶活性的潜在有害影响。使用 API®ZYM 试验测量了消化酶的活性,并将结果与昆虫年龄组(幼虫、幼年成虫和成年成虫)和以前的长寿选择("长寿 "品系与 "野生 "品系)进行了比较。同时对所有消化酶进行的总体分析以及对单个酶亚群进行的分析表明,这两个因素及其相互作用显著地改变了家蝇消化酶的活性。最明显的影响与年龄因素有关。研究表明,成年幼年组最为敏感;与对照组相比,该组消化酶的活性普遍下降,而其余组对 GO 的反应强度则不尽相同。反过来,菌株间的比较也显示出对 GO 的不同反应模式。一般来说,"长寿昆虫 "菌株的对照酶活性高于 "野生菌株";然而,暴露于 GO 的菌株组的酶活性明显降低,而 "野生 "菌株的酶活性则保持相对稳定。此外,与其他年龄组相比,这种趋势在成年幼鼠中更为明显。GO 的潜在毒性需要进一步研究,主要侧重于肠道微生物群的组成和功能。
Adult young as the fragile ontogenetic stage of the house crickets dietary exposed to GO nanoparticles – digestive enzymes perspective
Graphene oxide nanoparticles (GO) (have promising properties for, electronics, energy, medicine, water purification, agriculture and food production industry. However, their potentially hazardous effects are still not satisfactorily recognized, so they are often included in the group of contaminants of emerging concern. Therefore, the aim of this investigation was to assess the potentially harmful effects of orally administered GO on the digestive enzyme activities of the house crickets Acheta domesticus. The activity of digestive enzymes was measured using the API®ZYM test and the results were compared in relation to the insect age group (Larvae, Adult Young and Adult Mature) and previous selection towards longevity (‘long living’ vs ‘wild’ strains). General analysis, encompassing all digestive enzymes simultaneously, as well as an analysis performed for individual enzyme subgroups, revealed that both factors and their interactions significantly modified the activity of digestive enzymes of A. domesticus. The most pronounced effect was connected with the age factor. The study revealed that the Adult Young age group was the most sensitive; in this group, the activity of digestive enzymes was, in general, decreased in comparison with the control group, while the remaining group did not respond to GO with a similar intensity. In turn, the inter-strain comparisons demonstrated different response patterns to the GO. The control enzymatic activity of the insects from long living’ strain was, in general, higher than in the ‘wild strain’; however, it was significantly decreased in the GO-exposed groups, while in the ‘wild’ strain, the activity remained relatively homogenous. Additionally, the tendency was more pronounced in Adult Young than in the remaining age groups. The potential toxicity of GO requires further investigation, mainly with a focus on the composition and functioning of gut microbiotas.
期刊介绍:
Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.