利用新型水平剪切流技术快速制造无细胞的各向异性胶原组织

IF 3 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY Materialia Pub Date : 2024-11-02 DOI:10.1016/j.mtla.2024.102281
Alessandra Grillo , Maria Caluianu , Augustin Barna , Avantika Mair , Arnau Garriga Casanovas , Vivek Mudera , Alvena Kureshi
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引用次数: 0

摘要

由于胶原蛋白的承重特性,胶原纤维的超微结构和组织对组织功能至关重要。目前用于制造对齐胶原组织等效物的技术利用细胞的收缩能力来重塑和对齐胶原纤维,或使用高度专业化的设备。本研究的目的是开发一种新颖、快速的方法,通过结合水平剪切流(HSFlow)和成熟的 RAFT 方法来去除水凝胶中多余的液体,从而制作细胞排列胶原蛋白片。对纤维和细胞排列的定量分析显示,HSFlow 与对照样品之间存在显著差异,与随机排列的 RAFT 对照组相比,细胞和胶原纤维都显示出沿剪切流方向排列。总之,我们开发了一种新颖而快速的技术,无需依赖细胞的收缩能力来重新排列胶原纤维,即可生成排列整齐的胶原蛋白平板。这种快速方法有望用于制造支架,以模拟再生医学中的各向异性组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Rapid biofabrication of cell-free, anisotropic collagen tissues using a novel horizontal shear flow technique
Ultrastructure and organisation of collagen fibres is essential to tissue function, due to the loadbearing properties of collagen. Current techniques used to create aligned collagen tissue equivalents use the contractile ability of cells to remodel and align collagen fibres or utilise highly specialised pieces of equipment. The aim of this study is to develop a novel and rapid method to produce acellular aligned collagen sheets by combining horizontal shear flow (HSFlow) and the established RAFT method to remove excess fluid from a hydrogel.
Force applied to the gel during the HSFlow process was measured to allow replication of the method. Quantification of fibres and cellular alignment revealed a significant difference between HSFlow and control samples, where both cells and collagen fibres showed alignment in the direction of shear flow, compared to the randomly aligned RAFT controls. Mechanical properties were also measured and revealed that HSFlow does not appear to improve the strength of the constructs despite the improved alignment, therefore further optimisation is needed to strengthen the constructs.
In conclusion, we developed a novel and rapid technique to generate flat sheets of aligned collagen without relying on the contractile ability of cells to rearrange collagen fibres. This rapid method has potential to be used in the fabrication of a scaffold to mimic anisotropic tissues for regenerative medicine.
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来源期刊
Materialia
Materialia MATERIALS SCIENCE, MULTIDISCIPLINARY-
CiteScore
6.40
自引率
2.90%
发文量
345
审稿时长
36 days
期刊介绍: Materialia is a multidisciplinary journal of materials science and engineering that publishes original peer-reviewed research articles. Articles in Materialia advance the understanding of the relationship between processing, structure, property, and function of materials. Materialia publishes full-length research articles, review articles, and letters (short communications). In addition to receiving direct submissions, Materialia also accepts transfers from Acta Materialia, Inc. partner journals. Materialia offers authors the choice to publish on an open access model (with author fee), or on a subscription model (with no author fee).
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