ABI3 可调节 ABI1 的功能,从而控制主根伸长区的细胞长度。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-04 DOI:10.1111/tpj.17121
Saptarshi Datta, Drishti Mandal, Sicon Mitra, Swarnavo Chakraborty, Ronita Nag Chaudhuri
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引用次数: 0

摘要

胚后主根的生长实际上是多种激素信号途径相互作用的结果。在这里,我们发现 ABA 响应转录因子 ABI3 通过调控 JA 信号分子 JAZ1 和 ABA 响应因子 ABI1 来控制主根的生长。在 ABI3 缺失的情况下,主根伸长区缩短,细胞长度显著减少。表达分析和基于 ChIP 的检测表明,ABI3 通过占据 JAZ1 的上游调控序列并富集抑制性组蛋白修饰标记 H3K27 三甲基化,从而阻断 RNAPII 的占据,从而负向调控 JAZ1 的表达。先前的研究表明,JAZ1 与 ABA 信号传导过程中起作用的蛋白磷酸酶 2C ABI1 相互作用。我们的研究结果表明,在没有 ABI3 的情况下,当 JAZ1 表达水平较高时,与 JAZ1 缺失或 ABI3 过表达时相比,ABI1 蛋白显示出更高的稳定性。因此,在 abi3-6 突变体中,由于 ABI1 的稳定性提高,质膜 H+-ATPase(AHA2)的磷酸化减少。HPTS 染色进一步表明,与野生型和 ABI3 过表达幼苗相比,abi3-6 根细胞凋亡体的质子化程度降低。这种质子挤出的阻碍对主根伸长区的细胞长度产生了负面影响。因此,ABI3 通过影响根细胞凋亡体的 ABI1 依赖性质子化来控制主根的细胞伸长。总之,ABI3 控制 JAZ1 的表达,进而调节 ABI1 的功能,从而在主根生长过程中调节伸长区的细胞长度。
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ABI3 regulates ABI1 function to control cell length in primary root elongation zone.

Post-embryonic primary root growth is effectively an interplay of several hormone signalling pathways. Here, we show that the ABA-responsive transcription factor ABI3 controls primary root growth through the regulation of JA signalling molecule JAZ1 along with ABA-responsive factor ABI1. In the absence of ABI3, the primary root elongation zone is shortened with significantly reduced cell length. Expression analyses and ChIP-based assays indicate that ABI3 negatively regulates JAZ1 expression by occupying its upstream regulatory sequence and enriching repressive histone modification mark H3K27 trimethylation, thereby occluding RNAPII occupancy. Previous studies have shown that JAZ1 interacts with ABI1, the protein phosphatase 2C, that works during ABA signalling. Our results indicate that in the absence of ABI3, when JAZ1 expression levels are high, the ABI1 protein shows increased stability, compared to when JAZ1 is absent, or ABI3 is overexpressed. Consequently, in the abi3-6 mutant, due to the higher stability of ABI1, reduced phosphorylation of plasma membrane H+-ATPase (AHA2) occurs. HPTS staining further indicated that abi3-6 root cell apoplasts show reduced protonation, compared to wild-type and ABI3 overexpressing seedlings. Such impeded proton extrusion negatively affects cell length in the primary root elongation zone. ABI3 therefore controls cell elongation in the primary root by affecting the ABI1-dependent protonation of root cell apoplasts. In summary, ABI3 controls the expression of JAZ1 and in turn modulates the function of ABI1 to regulate cell length in the elongation zone during primary root growth.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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