Bo Li, Yan Ge, Jianguang Liang, Zhichun Zhu, Biqin Chen, Dan Li, Yingping Zhuang, Zejian Wang
{"title":"精确调节特定耗氧量，加强水螅的 CoQ10 生物合成。","authors":"Bo Li, Yan Ge, Jianguang Liang, Zhichun Zhu, Biqin Chen, Dan Li, Yingping Zhuang, Zejian Wang","doi":"10.1186/s40643-024-00813-0","DOIUrl":null,"url":null,"abstract":"<p><p>Coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides (R. sphaeroides). Oxygen supply was identified as a bottleneck for improving CoQ<sub>10</sub> yield in R. sphaeroides. In this study, a precise regulation strategy based on dielectric spectroscopy (DS) was applied to further improve CoQ<sub>10</sub> biosynthesis by R. sphaeroide. First, a quantitative response model among viable cells, cell morphology, and oxygen uptake rate (OUR) was established. DS could be used to detect viable R. sphaeroides cells, and the relationship among cell morphology, CoQ<sub>10</sub> biosynthesis, and OUR was found to be significant. Based on this model, the online specific oxygen consumption rate (Q<sub>O2</sub>) control strategy was successfully applied to the CoQ<sub>10</sub> fermentation process. Q<sub>O2</sub> controlled at 0.07 ± 0.01 × 10<sup>- 7</sup>mmol/cell/h was most favorable for CoQ<sub>10</sub> biosynthesis, resulting in a 28.3% increase in CoQ<sub>10</sub> production. Based on the multi-parameters analysis and online Q<sub>O2</sub> control, a precise online nutrient feeding strategy was established using conductivity detected by DS. CoQ<sub>10</sub> production was improved by 35%, reaching 3384 mg/L in 50 L bioreactors. This online control strategy would be effectively applied for improving industrial CoQ<sub>10</sub> production, and the precise fermentation control strategy could also be applied to other fermentation process.</p>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534906/pdf/","citationCount":"0","resultStr":"{\"title\":\"Precise regulating the specific oxygen consumption rate to strengthen the CoQ<sub>10</sub> biosynthesis by Rhodobater sphaeroides.\",\"authors\":\"Bo Li, Yan Ge, Jianguang Liang, Zhichun Zhu, Biqin Chen, Dan Li, Yingping Zhuang, Zejian Wang\",\"doi\":\"10.1186/s40643-024-00813-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides (R. sphaeroides). Oxygen supply was identified as a bottleneck for improving CoQ<sub>10</sub> yield in R. sphaeroides. In this study, a precise regulation strategy based on dielectric spectroscopy (DS) was applied to further improve CoQ<sub>10</sub> biosynthesis by R. sphaeroide. First, a quantitative response model among viable cells, cell morphology, and oxygen uptake rate (OUR) was established. DS could be used to detect viable R. sphaeroides cells, and the relationship among cell morphology, CoQ<sub>10</sub> biosynthesis, and OUR was found to be significant. Based on this model, the online specific oxygen consumption rate (Q<sub>O2</sub>) control strategy was successfully applied to the CoQ<sub>10</sub> fermentation process. Q<sub>O2</sub> controlled at 0.07 ± 0.01 × 10<sup>- 7</sup>mmol/cell/h was most favorable for CoQ<sub>10</sub> biosynthesis, resulting in a 28.3% increase in CoQ<sub>10</sub> production. Based on the multi-parameters analysis and online Q<sub>O2</sub> control, a precise online nutrient feeding strategy was established using conductivity detected by DS. CoQ<sub>10</sub> production was improved by 35%, reaching 3384 mg/L in 50 L bioreactors. This online control strategy would be effectively applied for improving industrial CoQ<sub>10</sub> production, and the precise fermentation control strategy could also be applied to other fermentation process.</p>\",\"PeriodicalId\":9067,\"journal\":{\"name\":\"Bioresources and Bioprocessing\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-11-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534906/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresources and Bioprocessing\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1186/s40643-024-00813-0\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresources and Bioprocessing","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s40643-024-00813-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Precise regulating the specific oxygen consumption rate to strengthen the CoQ10 biosynthesis by Rhodobater sphaeroides.
Coenzyme Q10 (CoQ10) is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides (R. sphaeroides). Oxygen supply was identified as a bottleneck for improving CoQ10 yield in R. sphaeroides. In this study, a precise regulation strategy based on dielectric spectroscopy (DS) was applied to further improve CoQ10 biosynthesis by R. sphaeroide. First, a quantitative response model among viable cells, cell morphology, and oxygen uptake rate (OUR) was established. DS could be used to detect viable R. sphaeroides cells, and the relationship among cell morphology, CoQ10 biosynthesis, and OUR was found to be significant. Based on this model, the online specific oxygen consumption rate (QO2) control strategy was successfully applied to the CoQ10 fermentation process. QO2 controlled at 0.07 ± 0.01 × 10- 7mmol/cell/h was most favorable for CoQ10 biosynthesis, resulting in a 28.3% increase in CoQ10 production. Based on the multi-parameters analysis and online QO2 control, a precise online nutrient feeding strategy was established using conductivity detected by DS. CoQ10 production was improved by 35%, reaching 3384 mg/L in 50 L bioreactors. This online control strategy would be effectively applied for improving industrial CoQ10 production, and the precise fermentation control strategy could also be applied to other fermentation process.
期刊介绍：
Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology
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