Amazonins:来自内生菌株金黄毛霉的新 Peptaibol 序列。

IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemistry & Biodiversity Pub Date : 2024-11-04 DOI:10.1002/cbdv.202400611
Gleucinei Dos Santos Castro, Thiago Fernandes Sousa, Ingride Jarline Santos da Silva, Débora Sena Raposo, José Carlos Ipuchima da Silva, Evelyn Peñaloza, Rafael Garrett, Michel Eduardo Beleza Yamagishi, Gilvan Ferreira da Silva, Hector Henrique Ferreira Koolen
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引用次数: 0

摘要

通过基因组挖掘、高效液相色谱耦合高分辨串联质谱(LC-MS/MS)和分子网络,研究人员从内生真菌Trichoderma amazonicum中鉴定出了三种新的14残基肽酚推定序列,并将其命名为amazonins I-III。对 T. amazonicum 基因组的生物信息学分析发现了 63 个与次生代谢物有关的生物合成基因(BGC)群,其中包括一个负责生物合成所发现的肽序列的非核糖体肽合成酶。通过对腺苷酸化结构域的分析以及对 MS/MS 图谱的人工解读,对新的多肽序列进行了广泛的注释。生物信息学工具与 LC-MS/MS 的结合为表征和鉴定新的七叶皂苷序列提供了更好的机会。因此,对亚马逊地区毛霉菌产生的七叶酚的生产和特征进行研究的重要性就凸显出来了。
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Amazonins: New Peptaibol Sequences from an Endophytic Strain of Trichoderma amazonicum.

Three new putative sequences of 14-residue peptaibols, named amazonins I-III were characterized from the endophytic fungus Trichoderma amazonicum via genome mining, high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS), and molecular networking. Bioinformatic analysis of the T. amazonicum genome assembly revealed 63 clusters of biosynthetic genes (BGCs) related to secondary metabolites, including a nonribosomal peptide synthetase accountable for the biosynthesis of the discovered peptide sequences. Analysis of the adenylation domains, along with manual interpretation of MS/MS spectra, allowed extensive annotation of the new peptaibol sequences. The combination of bioinformatic tools and LC-MS/MS provides a better opportunity to characterize and identify new peptaibol sequences. Thus, the importance of studies on the production and characterization of peptaibols produced by Trichoderma species from the Amazon region is highlighted.

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来源期刊
Chemistry & Biodiversity
Chemistry & Biodiversity 环境科学-化学综合
CiteScore
3.40
自引率
10.30%
发文量
475
审稿时长
2.6 months
期刊介绍: Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.
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