铜的获取对根部感染维管束枯萎病真菌的植物定殖和毒力至关重要。

IF 5.5 1区 医学 Q1 MICROBIOLOGY PLoS Pathogens Pub Date : 2024-11-04 DOI:10.1371/journal.ppat.1012671
Rafael Palos-Fernández, María Victoria Aguilar-Pontes, Gema Puebla-Planas, Harald Berger, Lena Studt-Reinhold, Joseph Strauss, Antonio Di Pietro, Manuel Sánchez López-Berges
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引用次数: 0

摘要

植物病原真菌会给农业造成毁灭性损失,而且难以控制。人们对这些生物在宿主环境中生长期间如何获取微量营养元素仍然知之甚少。在这里,我们发现铜获取机制的有效调控对于土传子囊菌 Fusarium oxysporum 的植物定殖和毒力至关重要,Fusarium oxysporum 是 150 多种作物维管束枯萎病的病原菌。通过结合使用 RNA-seq 和 ChIP-seq 技术,我们确定了转录调节因子 Mac1 在激活铜缺乏响应基因中的直接作用,其中许多基因在植物感染期间被诱导。缺失 Mac1 会影响 F. oxysporum 在低铜条件下的生长,并削弱其在番茄植株和无脊椎动物宿主 Galleria mellonella 上的致病性。重要的是,过表达编码铜还原酶和铜转运体的两个 Mac1 靶基因足以恢复 mac1 突变体背景下的致病性。我们的研究结果证实了铜还原和吸收在真菌感染植物过程中的作用,并揭示了保护农作物免受植物病原体侵害的新方法。
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Copper acquisition is essential for plant colonization and virulence in a root-infecting vascular wilt fungus.

Plant pathogenic fungi provoke devastating agricultural losses and are difficult to control. How these organisms acquire micronutrients during growth in the host environment remains poorly understood. Here we show that efficient regulation of copper acquisition mechanisms is crucial for plant colonization and virulence in the soilborne ascomycete Fusarium oxysporum, the causal agent of vascular wilt disease in more than 150 different crops. Using a combination of RNA-seq and ChIP-seq, we establish a direct role of the transcriptional regulator Mac1 in activation of copper deficiency response genes, many of which are induced during plant infection. Loss of Mac1 impaired growth of F. oxysporum under low copper conditions and abolishes pathogenicity on tomato plants and on the invertebrate animal host Galleria mellonella. Importantly, overexpression of two Mac1 target genes encoding a copper reductase and a copper transporter was sufficient to restore virulence in the mac1 mutant background. Our results establish a previously unrecognized role of copper reduction and uptake in fungal infection of plants and reveal new ways to protect crops from phytopathogens.

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来源期刊
PLoS Pathogens
PLoS Pathogens MICROBIOLOGY-PARASITOLOGY
自引率
3.00%
发文量
598
期刊介绍: Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.
期刊最新文献
Suppressing neutrophil itaconate production attenuates Mycoplasma pneumoniae pneumonia. Adaptation of Candida albicans to specific host environments by gain-of-function mutations in transcription factors. CD4+ but not CD8+ T cells are required for protection against severe guinea pig cytomegalovirus infections. Copper acquisition is essential for plant colonization and virulence in a root-infecting vascular wilt fungus. Tissue specific innate immune responses impact viral infection in Drosophila.
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