Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice

Optoacoustic (OA) tomography is a powerful noninvasive preclinical imaging tool enabling high resolution whole-body visualization of biodistribution and dynamics of molecular agents. The technique yet lacks endogenous soft-tissue contrast, which often hampers anatomical navigation. Herein, we devise spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography for concurrent OA and pulse-echo ultrasound (US) imaging of whole mice. To this end, a spherical array transducer featuring a central curvilinear segment is employed. Full rotation of the array renders transverse US and OA views, while additional translation facilitates volumetric whole-body imaging with high spatial resolution down to 150 µm and 110 µm in the OA and US modes, respectively. OA imaging revealed blood-filled, vascular organs like heart, liver, spleen, kidneys, and surrounding vasculature, whilst complementary details of bones, lungs, and skin boundaries were provided by the US. The dual-modal capability of SVOPUS for label-free imaging of tissue morphology and function is poised to facilitate pharmacokinetic studies, disease monitoring, and image-guided therapies.