{"title":"利用亚 1 纳米 CuO-PMA 纳米片增强化学发光,实现对槲皮素的灵敏检测","authors":"Kuangjun Li, Chunxia Huang, Tianyou Chen, Suxing Jiao, Jing Wu","doi":"10.1016/j.aca.2024.343395","DOIUrl":null,"url":null,"abstract":"<h3>Background</h3>Chemiluminescence (CL) analysis, characterized by its simple instrumentation, high signal-to-noise ratio, wide linear range, and minimal background interference, has garnered increasing attention from researchers. Nanomaterials (NMs) have been explored to enhance CL intensity. Notably, sub-1 nanometer scale NMs are considered to hold significant untapped potential due to their size effects. The application of these sub-1 nanometer NMs in enhancing CL is anticipated to yield favorable results. Additionally, the low water solubility and bioavailability of quercetin glycosides lead to their presence in bodily fluids at only trace levels, highlighting the urgent need for efficient and rapid detection methods.<h3>Results</h3>In this work, phosphomolybdic acid (PMA) was incorporated into CuO to synthesize sub-1 nanometer CuO-PMA nanosheets (SNSs) using a cluster-core co-assembly strategy. Conformational and structural characterization confirmed the successful synthesis of these nanosheets. The CuO-PMA SNSs were employed to enhance the CL emission of the luminol-H<sub>2</sub>O<sub>2</sub> system, resulting in an increase of over 1000 times. The catalytic properties of CuO-PMA SNSs significantly facilitated the decomposition of H<sub>2</sub>O<sub>2</sub>, leading to an enhanced production of reactive oxygen species, which in turn induced the CL enhancement. Given that the antioxidant effect of quercetin would consume the reactive oxygen species generated during the catalysis, a decrease in CL intensity was anticipated. A CL sensor for quercetin detection was developed based on the CuO-PMA SNSs-luminol-H<sub>2</sub>O<sub>2</sub> system, demonstrating a strong linear relationship (R<sup>2</sup> = 0.9969) and a low detection limit of 0.31 nM.<h3>Significance</h3>This research provides a strategy to enhance the CL intensity of the luminol-H<sub>2</sub>O<sub>2</sub> system by using CuO-PMA SNSs, offering a highly sensitive assay for detecting quercetin concentrations. The method is characterized as a simple and cost-effective analytical strategy making CL analysis very attractive for chemical analysts.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced chemiluminescence with sub-1 nanometer CuO-PMA nanosheets for the sensitive detection of quercetin\",\"authors\":\"Kuangjun Li, Chunxia Huang, Tianyou Chen, Suxing Jiao, Jing Wu\",\"doi\":\"10.1016/j.aca.2024.343395\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3>Background</h3>Chemiluminescence (CL) analysis, characterized by its simple instrumentation, high signal-to-noise ratio, wide linear range, and minimal background interference, has garnered increasing attention from researchers. Nanomaterials (NMs) have been explored to enhance CL intensity. Notably, sub-1 nanometer scale NMs are considered to hold significant untapped potential due to their size effects. The application of these sub-1 nanometer NMs in enhancing CL is anticipated to yield favorable results. Additionally, the low water solubility and bioavailability of quercetin glycosides lead to their presence in bodily fluids at only trace levels, highlighting the urgent need for efficient and rapid detection methods.<h3>Results</h3>In this work, phosphomolybdic acid (PMA) was incorporated into CuO to synthesize sub-1 nanometer CuO-PMA nanosheets (SNSs) using a cluster-core co-assembly strategy. Conformational and structural characterization confirmed the successful synthesis of these nanosheets. The CuO-PMA SNSs were employed to enhance the CL emission of the luminol-H<sub>2</sub>O<sub>2</sub> system, resulting in an increase of over 1000 times. The catalytic properties of CuO-PMA SNSs significantly facilitated the decomposition of H<sub>2</sub>O<sub>2</sub>, leading to an enhanced production of reactive oxygen species, which in turn induced the CL enhancement. Given that the antioxidant effect of quercetin would consume the reactive oxygen species generated during the catalysis, a decrease in CL intensity was anticipated. A CL sensor for quercetin detection was developed based on the CuO-PMA SNSs-luminol-H<sub>2</sub>O<sub>2</sub> system, demonstrating a strong linear relationship (R<sup>2</sup> = 0.9969) and a low detection limit of 0.31 nM.<h3>Significance</h3>This research provides a strategy to enhance the CL intensity of the luminol-H<sub>2</sub>O<sub>2</sub> system by using CuO-PMA SNSs, offering a highly sensitive assay for detecting quercetin concentrations. The method is characterized as a simple and cost-effective analytical strategy making CL analysis very attractive for chemical analysts.\",\"PeriodicalId\":240,\"journal\":{\"name\":\"Analytica Chimica Acta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-11-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytica Chimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.aca.2024.343395\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.aca.2024.343395","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Enhanced chemiluminescence with sub-1 nanometer CuO-PMA nanosheets for the sensitive detection of quercetin
Background
Chemiluminescence (CL) analysis, characterized by its simple instrumentation, high signal-to-noise ratio, wide linear range, and minimal background interference, has garnered increasing attention from researchers. Nanomaterials (NMs) have been explored to enhance CL intensity. Notably, sub-1 nanometer scale NMs are considered to hold significant untapped potential due to their size effects. The application of these sub-1 nanometer NMs in enhancing CL is anticipated to yield favorable results. Additionally, the low water solubility and bioavailability of quercetin glycosides lead to their presence in bodily fluids at only trace levels, highlighting the urgent need for efficient and rapid detection methods.
Results
In this work, phosphomolybdic acid (PMA) was incorporated into CuO to synthesize sub-1 nanometer CuO-PMA nanosheets (SNSs) using a cluster-core co-assembly strategy. Conformational and structural characterization confirmed the successful synthesis of these nanosheets. The CuO-PMA SNSs were employed to enhance the CL emission of the luminol-H2O2 system, resulting in an increase of over 1000 times. The catalytic properties of CuO-PMA SNSs significantly facilitated the decomposition of H2O2, leading to an enhanced production of reactive oxygen species, which in turn induced the CL enhancement. Given that the antioxidant effect of quercetin would consume the reactive oxygen species generated during the catalysis, a decrease in CL intensity was anticipated. A CL sensor for quercetin detection was developed based on the CuO-PMA SNSs-luminol-H2O2 system, demonstrating a strong linear relationship (R2 = 0.9969) and a low detection limit of 0.31 nM.
Significance
This research provides a strategy to enhance the CL intensity of the luminol-H2O2 system by using CuO-PMA SNSs, offering a highly sensitive assay for detecting quercetin concentrations. The method is characterized as a simple and cost-effective analytical strategy making CL analysis very attractive for chemical analysts.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.