OriT 长度与 RP4 介导的大肠杆菌与革兰氏阳性菌的连接效率之间的关系。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-11-05 DOI:10.1093/bbb/zbae159

Yuta Fukada, Shunsuke Inomata, Masakazu Kataoka

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引用次数: 0

摘要

在 RP4 连接中，大约 350 bp 的转运起源（oriT）是转运所必需的。在这个 oriT 中，有转移相关蛋白 TraI、TraK 和 TraJ 的结合区。我们研究了在大肠杆菌、S. lividans 和 B. subtilis 中删除 oriT 中每个蛋白结合区对转运效率的影响。在所有物种中，TraI 结合区的缺失都会完全取消转运。部分缺失 TraK 结合区在靶向时影响很小，但在靶向枯草芽孢杆菌时影响效率。在大肠杆菌和枯草杆菌中，TraJ 结合区的缺失完全取消了转移，但在 S. lividans 中仅降低了效率。这是第一份研究 oriT 内各区域对 S. lividans 和 B. subtilis 转化效率影响的报告，表明在以革兰氏阳性菌为靶标时，有效 RP4 连接所需的 oriT 长度是不同的。

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Relationship between oriT length and efficiency of RP4-mediated conjugation from E. coli to gram-positive bacteria.

In RP4 conjugation, approximately 350 bp of the origin of transfer (oriT) is required for transfer. Within this oriT, there are binding regions for the transfer-related proteins TraI, TraK, and TraJ. We investigated the influence of deleting each protein binding region within oriT on transfer efficiency in E. coli, S. lividans, and B. subtilis. The deletion of the TraI binding region completely abolished transfer in all species. The partial deletion of the TraK binding region had a minimal impact when targeting but affected efficiency when targeting B. subtilis. The deletion of the TraJ binding region completely abolished transfer in E. coli and B. subtilis but only reduced efficiency in S. lividans. This is the first report to investigate the influence of each region within oriT on transfer efficiency in S. lividans and B. subtilis, suggesting that the length of oriT required for effective RP4 conjugation varies when targeting Gram-positive bacteria.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.