高海拔缺氧会改变女性卵泡代谢组和卵母细胞发育潜能。

IF 2.1 3区 医学 Q2 OBSTETRICS & GYNECOLOGY Archives of Gynecology and Obstetrics Pub Date : 2024-11-06 DOI:10.1007/s00404-024-07695-9
Zhengfang Xiong, Xiaolei Liu, Qingdi Wang
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引用次数: 0

摘要

目的:为了探讨高海拔缺氧对卵母细胞发育和受精潜能微环境的影响,我们比较了生活在不同海拔地区的妇女卵泡液的代谢组学模式,并追踪其卵母细胞的成熟和后续发育过程:方法:共收集了 315 例临床病例,并根据居住海拔高度将其分为三组:低海拔(2800 米)组 138 例。对临床结果进行统计,包括激素水平、卵母细胞成熟度、体外受精和胚胎发育。同时,利用超高效液相色谱法和高分辨质谱法对不同组别妇女的卵泡液进行了代谢组学分析,并通过 KEGG 通路对差异代谢物进行了分析:结果:临床数据表明,不同组的身体状况和生殖激素分泌情况相似。虽然采用了个性化的促性腺激素释放激素策略,但前房卵泡数和获得的卵母细胞数并未受到居住海拔高度变化的影响。在体外培养中,高海拔组的成熟率、受精率和卵裂率与其他组进行了比较。但是,优质胚胎率、形成囊胚率和可用囊胚率随着居住海拔的升高而逐渐降低。代谢组分析确定了女性卵泡液中的 1193 种代谢物。差异分析表明,随着居住海拔的升高,卵泡液中的代谢成分发生了显著变化。这些差异代谢物与氨基酸代谢、蛋白质消化吸收、卵母细胞减数分裂和类固醇生物合成密切相关:结论:居住海拔会改变卵泡液的微环境,从而损害卵母细胞的发育潜能。这项研究为女性卵母细胞和胚胎发育研究提供了诊断依据和治疗目标。
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High-altitude hypoxia exposure alters follicular metabolome and oocyte developmental potential in women.

Purpose: To explore the effects of high-altitude hypoxia on the microenvironment of oocyte development and fertilization potential, we compared the metabolomic patterns of follicular fluid from women living in different altitude areas and traced their oocyte maturation and subsequent development.

Methods: A total of 315 clinical cases were collected and divided into three groups according to their residence altitudes: 138 cases in low-altitude (< 2300 m) group, 100 cases in middle-altitude (2300-2800 m) group and 77 cases in high-altitude (> 2800 m) group. The clinical outcomes were statistically estimated, including hormonal level, oocyte maturation, in vitro fertilization, and embryo development. Meanwhile, a metabolomic analysis was performed on the follicular fluid of women from different groups using ultra-high-performance liquid chromatography and high-resolution mass spectrometry and differential metabolites were analyzed through the KEGG pathway.

Results: The clinical data indicated that the physical condition and reproductive hormone secretion were similar among different groups. Although personalized gonadotropin-releasing hormone strategies were applied, the numbers of antral follicles and obtained oocytes were not impacted by the residence altitude change. In in vitro culture, the maturing rate, fertility rate and cleavage rate of high-altitude group were compared with the other groups. However, the rates of high-quality embryo, formative blastocyst, and available blastocyst were gradually decreased with the rise of residence altitude. Metabolome analysis identified 1193 metabolites in female follicular fluid. Differential analysis indicated that metabolic components in follicular fluid were remarkably changed with the elevation of residence altitude. These differential metabolites were closely related with amino acid metabolism, protein digestion and absorption, oocyte meiosis and steroid biosynthesis.

Conclusion: The residence altitude alters the microenvironment of follicular fluid, which could damage the oocyte developmental potential. This study provides diagnostic basis and therapeutic targets for research on female oocyte and embryo development.

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来源期刊
CiteScore
4.70
自引率
15.40%
发文量
493
审稿时长
1 months
期刊介绍: Founded in 1870 as "Archiv für Gynaekologie", Archives of Gynecology and Obstetrics has a long and outstanding tradition. Since 1922 the journal has been the Organ of the Deutsche Gesellschaft für Gynäkologie und Geburtshilfe. "The Archives of Gynecology and Obstetrics" is circulated in over 40 countries world wide and is indexed in "PubMed/Medline" and "Science Citation Index Expanded/Journal Citation Report". The journal publishes invited and submitted reviews; peer-reviewed original articles about clinical topics and basic research as well as news and views and guidelines and position statements from all sub-specialties in gynecology and obstetrics.
期刊最新文献
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