利用电感耦合等离子体质谱法分析人体全血、血清和尿液中 33 种元素的快速前向稀释-拍摄多元素法:用于临床诊断和生物监测的简化方法。

IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Journal of Analytical Methods in Chemistry Pub Date : 2024-08-20 eCollection Date: 2024-01-01 DOI:10.1155/2024/9944995
Sandra Huber, Jörg Michel, Maurice Reijnen, Maria Averina, Bjørn Bolann, Jon Øyvind Odland, Solrunn Hansen, Jan Brox
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引用次数: 0

摘要

人体基质中有毒和必需元素的分析用于临床诊断和不同人群的生物监测,以研究相关的健康结果。这项工作旨在开发快速可靠的方法,用于分析液态人体基质(如全血、血清和尿液)中的多种元素,并针对三种基质和不同的分析需求采用相似的设置。针对 33 种元素(即 Ag、Al、As、B、Ba、Be、Bi、Cd、Ce、Co、Cr、Cu、Hg、I、Li、Mn、Mo、Ni、Pb、Pd、Pt、Sb、Se、Sn、Sr、Te、Th、Tl、U、V、W、Zn 和 Zr)开发了一种简便快捷的稀释-拍摄法。用碱性试剂将 200 µL 样品材料稀释至总体积为 4 mL。样品稀释和基质匹配校准标准的制备在 48 孔板中通过自动液体处理机进行。稀释后的样品在配备了 ESI-FAST SC2DX 自动进样器的 Perkin Elmer NexIon 300D ICP-MS 仪器上进行电感耦合等离子体质谱分析,采用动能鉴别模式,以 4.8 mL 或 5.7 mL 的氦气作为池气,射频发生器功率为 1600 W。方法验证结果表明,外部质量评估系统对新鲜人体样本的检测具有良好的准确性,测量浓度在指定浓度范围内。大多数元素的精确度和重现性良好,全血的变异系数分别低于或远低于 8%和 15%,血清的变异系数分别低于或远低于 8%和 10%,尿液的变异系数分别低于或远低于 10%和 10%。所开发的试剂和仪器设置适用于所有三种基质。这就最大限度地降低了在不同样品基质之间切换分析时出现人为错误的风险,并能在需要时在一天内快速、简便地分析全血、血清和尿液。随着时间的推移,该方法表现出很强的稳定性,能够经受住工作溶液和样品制备、仪器分析和设置方面的细微变化。对人体真实样本的分析表明,该方法适用于全血、血清和尿液中 33 种有毒和必需元素的分析,其浓度可用于临床诊断和生物监测。
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A Fast-Forward Dilute-and-Shoot Multielement Method for Analysis of 33 Elements in Human Whole Blood, Serum, and Urine by Inductively Coupled Plasma Mass Spectrometry: A Streamlined Approach for Clinical Diagnostic and Biomonitoring.

The analysis of toxic and essential elements in human matrices is used in clinical diagnostics and for biomonitoring of different populations to study related health outcomes. This work aimed to develop fast and reliable methods for the analysis of a broad range of elements in liquid human matrices, such as whole blood, serum, and urine, with a similar setup for the three matrices and different analysis needs. An easy and fast-forward dilute-and-shoot method for 33 elements (i.e., Ag, Al, As, B, Ba, Be, Bi, Cd, Ce, Co, Cr, Cu, Hg, I, Li, Mn, Mo, Ni, Pb, Pd, Pt, Sb, Se, Sn, Sr, Te, Th, Tl, U, V, W, Zn, and Zr) was developed. 200 µL of either sample material was diluted with an alkaline reagent to a volume of 4 mL in total. Sample dilution and preparation of matrix-matched calibration standards were performed in 48-well plates by an automated liquid handler. Diluted samples were analyzed by inductively coupled plasma mass spectrometry on a Perkin Elmer NexIon 300D ICP-MS instrument equipped with an ESI-FAST SC2DX autosampler in kinetic energy discrimination mode with helium as cell gas at either 4.8 mL or 5.7 mL and 1600 W RF generator power. The method validation results showed good accuracy for fresh human samples from an external quality assessment scheme with measured concentrations within the assigned concentration ranges. Good precision and reproducibility for most elements were demonstrated with variation coefficients below or far below 8% and 15% for whole blood, 8% and 10% for serum, and 10% and 10% for urine, respectively. The developed reagent and instrumental setup were applicable to all three matrices. This minimizes the risk of human errors when switching between analyses of the different sample matrices and allows a rapid and easy analysis of whole blood, serum, and urine within one day if needed. The method demonstrated robustness over time, withstanding minor changes in the preparation of working solutions and samples, instrumental analysis, and setup. Analysis of human real samples showed the method's applicability for 33 toxic and essential elements in whole blood, serum, and urine and at concentrations relevant to clinical diagnostics as well as biomonitoring.

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来源期刊
Journal of Analytical Methods in Chemistry
Journal of Analytical Methods in Chemistry CHEMISTRY, ANALYTICAL-ENGINEERING, CIVIL
CiteScore
4.80
自引率
3.80%
发文量
79
审稿时长
6-12 weeks
期刊介绍: Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical. Subject areas include (but are by no means limited to): Separation Spectroscopy Mass spectrometry Chromatography Analytical Sample Preparation Electrochemical analysis Hyphenated techniques Data processing As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.
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