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Handgrip-Ring Structure Sensing Probe Assisted Multiple Signal Amplification Strategy for Sensitive and Label-Free Single-Stranded Nucleic Acid Analysis. 用于灵敏无标记单链核酸分析的手握环结构传感探针辅助多重信号放大策略。
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-10-18 eCollection Date: 2024-01-01 DOI: 10.1155/2024/6832856
Ying Ren, Yu He, Ping Li

Precise and efficient identification of single-stranded nucleic acids is crucial for both pathological research and early diagnosis of diseases, such as cancers. Therefore, we have devised a novel biosensor that utilizes an elegantly designed handgrip-ring structure sensing probe to enhance the detection sensitivity and reduce background signals. The handgrip-ring structure sensing probe combines ring padlock-based target recognition and hairpin structure probe-based signal amplification. The target sequences form a binding interaction with the ring padlock in the sensing probe, leading to the elongation of the single-stranded chain with the assistance of polymerase. This elongation step results in the release of the hairpin probe, triggering a signal amplification process. This design significantly minimized the potential discrepancies that may occur during the signal amplification process, hence bestowing the approach with a low level of background signals. By utilizing this innovative design, the current biosensor demonstrates a remarkable ability to detect miRNA with a limit as low as 376 aM and single-stranded DNA sequences with a limit as low as 45.3 aM. In addition, it possesses exceptional discrimination capabilities. The efficacy of this approach in diagnosing targets was also effectively proved by the rational redesign of the ring padlock.

精确高效地识别单链核酸对于病理研究和癌症等疾病的早期诊断至关重要。因此,我们设计了一种新型生物传感器,利用设计优雅的手握环结构传感探针来提高检测灵敏度并减少背景信号。手握环结构传感探针结合了基于挂锁环的目标识别和基于发夹结构探针的信号放大。目标序列与传感探针中的环状挂锁形成结合作用,在聚合酶的协助下导致单链的延伸。这一延伸步骤导致发夹探针的释放,引发信号放大过程。这种设计大大减少了信号放大过程中可能出现的偏差,因此这种方法的背景信号水平很低。通过采用这种创新设计,目前的生物传感器显示出卓越的能力,可检测低至 376 aM 的 miRNA 和低至 45.3 aM 的单链 DNA 序列。此外,它还具有卓越的分辨能力。对环形挂锁的合理重新设计也有效证明了这种方法在诊断目标方面的功效。
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引用次数: 0
H-Type Indices With Applications in Chemometrics: h-Accuracy Index for Evaluating and Comparing Errors in Analytical Chemistry. H 型指数在化学计量学中的应用:用于评估和比较分析化学误差的 h 精度指数。
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.1155/2024/1580359
Lu Xu, Xihui Bian, Qin Yang

Inspired by the popular h-index in bibliometrics, the h-accuracy index (HAI) was suggested as a new indicator to evaluate and compare errors in analytical chemistry. The HAI simultaneously considers the "trueness" of analytical measurements and the frequency of measurements with high "trueness". The HAI was defined as if the "trueness" of at most M% of the total measurements is no less than M%, the value of HAI will be M%, where a specific definition of "trueness" was given to compute the HAI. The range of the HAI was between zero and one. The HAI was applied to two examples as a new error index: (1) to evaluate and compare the analytical results by different methods/labs and (2) to evaluate and compare the prediction performances of different multivariate calibration models. Data analysis indicated that the HAI was a reasonable, robust, easy-to-compute, and comprehensive index for evaluating and comparing errors in analytical chemistry. Moreover, the HAI could provide the information about how many measurements are good.

受文献计量学中流行的 h 指数的启发,提出了 h 精确度指数(HAI)作为评估和比较分析化学误差的新指标。HAI 同时考虑了分析测量的 "真实度 "和高 "真实度 "测量的频率。HAI 的定义是:如果最多有 M% 的测量结果的 "真实度 "不低于 M%,则 HAI 值为 M%,其中给出了计算 HAI 的 "真实度 "的具体定义。HAI 的范围介于 0 和 1 之间。HAI 作为一种新的误差指数被应用于两个实例:(1) 评估和比较不同方法/实验室的分析结果;(2) 评估和比较不同多元校准模型的预测性能。数据分析表明,HAI 是评估和比较分析化学误差的一个合理、稳健、易于计算且全面的指标。此外,HAI 还能提供关于有多少测量结果是好的信息。
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引用次数: 0
Screening Antioxidant Components in Yiwei Decoction Using Spectrum-Effect Relationship and Network Pharmacology. 利用谱效关系和网络药理学筛选益卫煎膏中的抗氧化成分
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.1155/2024/5514265
Lei Zhang, Wei Zhu

Yiwei decoction (YWD) is a classic prescription with the function of nourishing stomach yin. In this study, the effective components of antioxidant activity of YWD and its possible mechanism were discussed from the point of view of spectral effect relationship and network pharmacology. Firstly, the fingerprints of 10 batches of YWD were established by UPLC-PDA technique, and the 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH) scavenging rate and total antioxidant capacity (T-AOC) were used as the indicators for antioxidant activity in vitro. Then, the spectral effect relationship between the fingerprint profiles and antioxidant capacity was analyzed through grey relational analysis (GRA) and orthogonal projections to latent structures (OPLS). In addition, network pharmacology was employed to predict the potential mechanisms of YWD in the treatment of antioxidant-related diseases. The spectrum-effect relationship indicated that three common peaks were likely to be the most decisive active components, identified as verbascoside, psoralen, and vitexin, respectively. Based on network pharmacology analysis, a total of 83 target genes shared by the active components and antioxidant-related diseases were collected. AKT1, HSP90AA1, SRC, CASP3, and MTOR were closely related to antioxidant therapy and considered as core therapeutic targets. The potential mechanisms of YWD were obtained through gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Finally, molecular docking simulations were conducted to evaluate the binding activities between the core therapeutic targets and corresponding compounds. The excellent core protein-compound complexes obtained by molecular docking were simulated by molecular dynamics simulation. The results showed that the active compounds had good binding ability with the selected targets. This study successfully identified the effective components of YWD and predicted the potential targets and pathways, which provided a new idea for the application of YWD in the treatment of antioxidant stress in the future. In addition, the potential active components provide valuable implications for drug screening of related diseases.

益卫煎是具有养胃阴作用的经典方剂。本研究从谱效关系和网络药理学的角度探讨了一味地黄丸抗氧化活性的有效成分及其可能机制。首先,采用UPLC-PDA技术建立了10批次玉竹的指纹图谱,并以1,1-二苯基-2-苦基肼(DPPH)自由基清除率和总抗氧化能力(T-AOC)作为体外抗氧化活性的指标。然后,通过灰色关系分析(GRA)和正交投影潜结构(OPLS)分析了指纹图谱与抗氧化能力之间的光谱效应关系。此外,还利用网络药理学预测了 YWD 治疗抗氧化相关疾病的潜在机制。光谱-效应关系表明,三个共同峰可能是最具决定性的活性成分,分别是马鞭草苷、补骨脂素和牡荆素。根据网络药理学分析,共收集到 83 个活性成分与抗氧化相关疾病共有的靶基因。其中AKT1、HSP90AA1、SRC、CASP3和MTOR与抗氧化治疗密切相关,被认为是核心治疗靶点。通过基因本体(GO)和京都基因和基因组百科全书(KEGG)获得了 YWD 的潜在机制。最后,进行了分子对接模拟,以评估核心治疗靶点与相应化合物之间的结合活性。分子对接获得的优良核心蛋白-化合物复合物通过分子动力学模拟进行了仿真。结果表明,活性化合物与所选靶点具有良好的结合能力。该研究成功鉴定了枸杞多糖的有效成分,并预测了其潜在靶点和途径,为今后枸杞多糖在抗氧化应激治疗中的应用提供了新思路。此外,潜在的活性成分还为相关疾病的药物筛选提供了宝贵的启示。
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引用次数: 0
Characterization of Volatile Organic Compounds and Aroma Sensory Properties in Yunnan Cigar. 云南雪茄挥发性有机化合物和香气感官特性的表征。
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.1155/2024/9583022
Yuping Wu, Haiyu Zhang, Wenyuan Wang, Guanghui Kong, Zaiming Li, Tikun Zhang, Miaochang Wang, Dong Yang, Chengming Zhang, Yongping Li, Jin Wang

To characterize volatile organic compounds (VOCs) and aromatic sensory properties in Yun cigar, 27 samples from four origins were analyzed using SPME-HS-GC/MS and sensory analysis. The investigation results were analyzed using principal component analysis (PCA), Fisher linear discriminant analysis (LDA), and Pearson correlation analysis. In Yunnan cigars, the content of nicotine and neophytadiene accounted for over 90% of the total VOC content. Nicotine was significantly positively correlated with neophytadiene and phytol. The cigars from four origins were clearly classified by the PCA of VOCs. Four region discrimination functions were established through the LDA of 14 compounds, and the validation accuracy was 100%. The sensory descriptors with the highest geometric mean were woody, roasted, fresh-sweet, bean, and scorched. Acetophenone, megastigmatrienone A, and thunbergene were positively correlated with multiple aroma descriptors, while nicotine was negatively correlated with multiple aroma descriptors.

为了确定云雪茄中挥发性有机化合物(VOC)和芳香感官特性的特征,我们采用 SPME-HS-GC/MS 和感官分析方法对来自四个产地的 27 个样品进行了分析。采用主成分分析法(PCA)、费雪线性判别分析法(LDA)和皮尔逊相关分析法对调查结果进行了分析。在云南雪茄中,尼古丁和新对二甲苯的含量占挥发性有机化合物总含量的 90% 以上。尼古丁与新茶二烯和植醇呈明显的正相关。通过挥发性有机化合物的 PCA 分析,可以对四个产地的雪茄进行清晰的分类。通过对 14 种化合物进行 LDA 分析,建立了四个区域判别函数,验证准确率为 100%。几何平均值最高的感官描述符是木质、烘烤、鲜甜、豆香和焦味。乙酰苯酮、巨薯三烯酮 A 和屯伯格烯与多种香气描述指标呈正相关,而尼古丁与多种香气描述指标呈负相关。
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引用次数: 0
Electrochemical Analysis of Methanol with Nafion-Coated Copper Oxide Nanoparticles. 使用 Nafion 涂层纳米氧化铜颗粒对甲醇进行电化学分析。
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-28 eCollection Date: 2024-01-01 DOI: 10.1155/2024/1809578
Pantipa Sawatmuenwai, Keerakit Kaewket, Kamonwad Ngamchuea

This work introduces a Nafion-coated copper(I) oxide nanoparticle electrode (Nafion/Cu2O/GC) designed for the electrochemical detection of methanol (CH3OH). The responses of the composite material toward CH3OH were enhanced by the selective permeation of CH3OH through the hydrophilic channels of the Nafion membrane in combination with the electroactivity of Cu2O nanoparticles. The sensor displayed a linear detection range of 0.33-100 mM CH3OH with a sensitivity of 0.17 μA·mM-1 and a detection limit (3 s/m) of 0.10 mM. It exhibited excellent reproducibility with a relative standard deviation of <5%. The sensor's practical applicability was demonstrated through recovery studies on hand sanitizer samples, achieving ca. 100% recovery. The sensor was further used to elucidate CH3OH adsorption on activated carbon, revealing that the process conforms to the Langmuir isotherm model.

本研究介绍了一种 Nafion 涂层纳米氧化铜(I)粒子电极(Nafion/Cu2O/GC),设计用于甲醇(CH3OH)的电化学检测。由于 CH3OH 通过 Nafion 膜亲水通道的选择性渗透与 Cu2O 纳米粒子的电活性相结合,增强了复合材料对 CH3OH 的响应。该传感器的线性检测范围为 0.33-100 mM CH3OH,灵敏度为 0.17 μA-mM-1,检测限(3 s/m)为 0.10 mM。该方法的重现性极佳,3OH 在活性炭上的吸附量相对标准偏差很小,表明该过程符合 Langmuir 等温线模型。
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引用次数: 0
Evaluation of Natural Radioactivity Level in Surface Soil from Bambasi District in Benishangul Gumuz Region, Ethiopia. 埃塞俄比亚贝尼尚古尔古穆兹地区班巴西区表层土壤中天然放射性水平的评估。
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-18 eCollection Date: 2024-01-01 DOI: 10.1155/2024/6633673
Yared Birhane Kidane, Tilahun Tesfaye Deressu, Guadie Degu Belete

The study assessed the concentration of natural radionuclides in soil samples from the Bambasi district in Ethiopia's Benishangul Gumuz region using a gamma-ray spectrometer equipped with a high-purity germanium (HPGe) detector. The measured activity concentrations of 238U, 232Th, and 40K in soil samples varied from 46.2 ± 2.25 to 88.49 ± 5.73 Bq/kg, 73.4 ± 4.12 to 119.65 ± 8.45 Bq/kg, and 176.78 ± 8.63 to 396.71 ± 25.39 Bq/kg, respectively. The average concentration of 238U and 232Th exceeded the recommended worldwide population weighted average values of 32.0 and 45.0 Bq/kg, respectively, while the average concentration of 40K was below the recommended value of 420.0 Bq/kg. The mean absorbed dose rate was calculated to be 91.6 ± 5.1 nGy/h, which is above the recommended safe value of 59 nGy/h. The average annual effective dose equivalents for indoor and outdoor exposure were determined to be 2.65 ± 0.14 mSv/y and 0.66 ± 0.1 mSv/y, respectively. The calculated mean values of the internal hazard index, external hazard index, and gamma index across all soil samples were 0.72 ± 0.05, 0.55 ± 0.02, and 0.72 ± 0.02, respectively, all below the recommended safe threshold of one. These findings suggest that the activity concentrations observed in the soil samples exceed safe levels, indicating the necessity for further investigation into radioactivity levels and epidemiological studies regarding potential hazards from high background radiation.

这项研究利用配备高纯锗(HPGe)探测器的伽马射线光谱仪,评估了埃塞俄比亚贝尼山古尔古穆兹地区班巴西区土壤样本中天然放射性核素的浓度。土壤样本中测得的 238U、232Th 和 40K 放射性活度浓度分别为 46.2 ± 2.25 至 88.49 ± 5.73 Bq/kg、73.4 ± 4.12 至 119.65 ± 8.45 Bq/kg、176.78 ± 8.63 至 396.71 ± 25.39 Bq/kg。238U 和 232Th 的平均浓度分别超过了 32.0 和 45.0 Bq/kg 的全球推荐人口加权平均值,而 40K 的平均浓度则低于 420.0 Bq/kg 的推荐值。计算得出的平均吸收剂量率为 91.6 ± 5.1 nGy/h,高于建议的安全值 59 nGy/h。室内和室外照射的年均有效剂量当量分别为 2.65 ± 0.14 mSv/y 和 0.66 ± 0.1 mSv/y。所有土壤样本的内部危害指数、外部危害指数和伽马指数的计算平均值分别为 0.72 ± 0.05、0.55 ± 0.02 和 0.72 ± 0.02,均低于建议的安全阈值 1。这些结果表明,在土壤样本中观察到的放射性浓度超过了安全水平,表明有必要对放射性水平进行进一步调查,并对高本底辐射的潜在危害进行流行病学研究。
{"title":"Evaluation of Natural Radioactivity Level in Surface Soil from Bambasi District in Benishangul Gumuz Region, Ethiopia.","authors":"Yared Birhane Kidane, Tilahun Tesfaye Deressu, Guadie Degu Belete","doi":"10.1155/2024/6633673","DOIUrl":"https://doi.org/10.1155/2024/6633673","url":null,"abstract":"<p><p>The study assessed the concentration of natural radionuclides in soil samples from the Bambasi district in Ethiopia's Benishangul Gumuz region using a gamma-ray spectrometer equipped with a high-purity germanium (HPGe) detector. The measured activity concentrations of <sup>238</sup>U, <sup>232</sup>Th, and <sup>40</sup>K in soil samples varied from 46.2 ± 2.25 to 88.49 ± 5.73 Bq/kg, 73.4 ± 4.12 to 119.65 ± 8.45 Bq/kg, and 176.78 ± 8.63 to 396.71 ± 25.39 Bq/kg, respectively. The average concentration of <sup>238</sup>U and <sup>232</sup>Th exceeded the recommended worldwide population weighted average values of 32.0 and 45.0 Bq/kg, respectively, while the average concentration of <sup>40</sup>K was below the recommended value of 420.0 Bq/kg. The mean absorbed dose rate was calculated to be 91.6 ± 5.1 nGy/h, which is above the recommended safe value of 59 nGy/h. The average annual effective dose equivalents for indoor and outdoor exposure were determined to be 2.65 ± 0.14 mSv/y and 0.66 ± 0.1 mSv/y, respectively. The calculated mean values of the internal hazard index, external hazard index, and gamma index across all soil samples were 0.72 ± 0.05, 0.55 ± 0.02, and 0.72 ± 0.02, respectively, all below the recommended safe threshold of one. These findings suggest that the activity concentrations observed in the soil samples exceed safe levels, indicating the necessity for further investigation into radioactivity levels and epidemiological studies regarding potential hazards from high background radiation.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11424854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishment of HPLC Fingerprints for Feiqizhong Tablets and Simultaneous Determination of Fourteen Constituents. 费奇中片高效液相色谱指纹图谱的建立及十四种成分的同时测定
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-18 eCollection Date: 2024-01-01 DOI: 10.1155/2024/7703951
Ting Yang, Fang Deng, Xuejun Yang, Shunxiang Li

Aim: To establish the HPLC fingerprints for Feiqizhong tablets and to simultaneously determine fourteen contents in Feiqizhong tablets.

Methods: The analysis of the methanol extract of this drug was performed on a 30°C thermostatic Shimadzu Shim-pack GIST-C18 column (250 mm × 4.6 mm, 5 um), with the mobile phase comprising acetonitrile (A) -0.1% phosphoric acid (B) flowing at 1.0 mL/min in a gradient elution manner (0-10 min, 88% A; 10-30 min, 88%-50% A; 30-60 min, 50%-30% A; 60-65 min, 30% A; 65-70 min, 30%-88% A; 70-75 min, 88% A), and the detection wavelength was fixed at 246 nm.

Results: There were one hundred and five common peaks in the HPLC fingerprints of twenty batches of samples with similarities of more than 0.953. Among them, fourteen major active components with a high response value in the chromatogram were selected for quantification, including eupalinolide B, tanshinone IIA, chlorogenic acid, psoralenoside, isopsoralenoside, icariin, amygdalin, caffeic acid, calycosin-7-O-β-D-glucoside, salvianolic acid B, quercetin, psoralen, isopsoralen, and bakuchiol. Fourteen constituents showed good linear relationships within their own ranges (r ≥ 0.9985), whose average recoveries were 90.71-106.14% with RSD values of 0.23-1.22%.

Conclusion: A rapid and effective method for the separation and detection of components in Feiqizhong tablets is established, which can provide a basis for quality control and quantitative detection.

目的:建立飞蓟中片的高效液相色谱指纹图谱,同时测定飞蓟中片中的 14 种成分:采用 30°C 恒温 Shimadzu Shim-pack GIST-C18 色谱柱(250 mm × 4.6 mm, 5 um),以乙腈(A)-0.1%磷酸(B)为流动相,流速为 1.0 mL/min,梯度洗脱(0.1 mL/min-1.0 mL/min)。流动相为乙腈(A)-0.1%磷酸(B),流速为 1.0 mL/min,梯度洗脱(0-10 min,88%A;10-30 min,88%-50%A;30-60 min,50%-30%A;60-65 min,30%A;65-70 min,30%-88%A;70-75 min,88%A),检测波长为 246 nm:结果:20 批样品的高效液相色谱指纹图谱共有 115 个共同峰,相似度大于 0.953。其中14种主要活性成分在色谱图中的响应值较高,被选中进行定量分析,包括玉竹内酯B、丹参酮IIA、绿原酸、补骨脂苷、异补骨脂苷、冰片苷、苦杏仁苷、咖啡酸、花萼苷-7-O-β-D-葡萄糖苷、丹参酚酸B、槲皮素、补骨脂烯、异补骨脂烯和白屈菜醇。14种成分在各自范围内呈良好的线性关系(r ≥ 0.9985),平均回收率为90.71-106.14%,RSD值为0.23-1.22%:建立了一种快速、有效的飞蓟中片成分分离检测方法,为飞蓟中片的质量控制和定量检测提供了依据。
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引用次数: 0
POCT Detection of Pseudomonas aeruginosa by PGM and Application of Preventing Nosocomial Infection of Bronchoscopy. 用 PGM 进行铜绿假单胞菌的 POCT 检测及在预防支气管镜检查中的非医院感染中的应用。
IF 2.6 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-05 DOI: 10.1155/2024/8062001
Tao Wen,Houqi Ning,Yinping Yang,Jinze Zhang
BackgroundThe primary pathogen responsible for bronchoscope contamination is Pseudomonas aeruginosa. Conventional techniques for bronchoscopy disinfection and pathogen identification methods are characterized by time-consuming and operation complexly. The objective of this research is to establish a prompt and precise method for the identification of Pseudomonas aeruginosa, with the ultimate goal of mitigating the risk of nosocomial infections linked to this pathogen.MethodsThe magnetic nanoparticles (MNPs) were synthesized in a single step, followed by the optimization of the coating process with antibodies and invertase to produce the bifunctionalized IMIc. Monoclonal antibodies were immobilized on microplates for the specific capture and enrichment of Pseudomonas aeruginosa. Upon the presence of Pseudomonas aeruginosa, the monoclonal antibodies, the test sample, and the IMIc formed sandwich structures. The subsequent addition of a sucrose solution allowed for the detection of glucose produced through invertase hydrolysis by a personal glucose meter, enabling quantitative assessment of Pseudomonas aeruginosa concentration.ResultsTEM image demonstrates that the MNPs exhibit a consistent spherical shape. NTA determined that the grain diameter of magnetic nanoparticles was 200 nm. FTIR spectrum revealed the successful modification of two carboxyl groups on the MNPs. The optimization of the incubation pH of the microplate-coated antibody was 7. The optimization of the incubation time of the microplate-coated antibody was 2 h. The optimization of the ligation pH for the polyclonal antibody was 5. Reaction times of polyclonal antibodies linked to magnetic beads was 1 h. The pH of invertase linked by magnetic beads was 4.ConclusionThis article presents a novel qualitative and quantitative immunoassay for point-of-care monitoring of P. aeruginosa utilizing PGM as a readout. The PGM represents a convenient and accurate quantitative detection method suitable for potential clinical diagnostic applications.
背景造成支气管镜污染的主要病原体是铜绿假单胞菌。传统的支气管镜消毒技术和病原体鉴定方法具有耗时长、操作复杂等特点。本研究的目的是建立一种快速、精确的铜绿假单胞菌鉴定方法,最终目标是降低与该病原体相关的院内感染风险。方法先一步合成磁性纳米粒子(MNPs),然后用抗体和转化酶优化涂层工艺,生产出双功能化的 IMIc。单克隆抗体被固定在微孔板上,用于特异性捕获和富集铜绿假单胞菌。铜绿假单胞菌存在时,单克隆抗体、测试样本和 IMIc 形成夹层结构。随后加入蔗糖溶液,通过个人葡萄糖计检测转化酶水解产生的葡萄糖,从而定量评估铜绿假单胞菌的浓度。NTA 确定磁性纳米粒子的晶粒直径为 200 nm。傅立叶变换红外光谱显示,MNPs 上的两个羧基被成功修饰。优化微孔板包被抗体的孵育 pH 值为 7;优化微孔板包被抗体的孵育时间为 2 小时;优化多克隆抗体的连接 pH 值为 5;与磁珠连接的多克隆抗体的反应时间为 1 小时;与磁珠连接的转化酶的 pH 值为 4。PGM 是一种方便、准确的定量检测方法,适用于潜在的临床诊断应用。
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引用次数: 0
A Fast-Forward Dilute-and-Shoot Multielement Method for Analysis of 33 Elements in Human Whole Blood, Serum, and Urine by Inductively Coupled Plasma Mass Spectrometry: A Streamlined Approach for Clinical Diagnostic and Biomonitoring. 利用电感耦合等离子体质谱法分析人体全血、血清和尿液中 33 种元素的快速前向稀释-拍摄多元素法:用于临床诊断和生物监测的简化方法。
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-08-20 eCollection Date: 2024-01-01 DOI: 10.1155/2024/9944995
Sandra Huber, Jörg Michel, Maurice Reijnen, Maria Averina, Bjørn Bolann, Jon Øyvind Odland, Solrunn Hansen, Jan Brox

The analysis of toxic and essential elements in human matrices is used in clinical diagnostics and for biomonitoring of different populations to study related health outcomes. This work aimed to develop fast and reliable methods for the analysis of a broad range of elements in liquid human matrices, such as whole blood, serum, and urine, with a similar setup for the three matrices and different analysis needs. An easy and fast-forward dilute-and-shoot method for 33 elements (i.e., Ag, Al, As, B, Ba, Be, Bi, Cd, Ce, Co, Cr, Cu, Hg, I, Li, Mn, Mo, Ni, Pb, Pd, Pt, Sb, Se, Sn, Sr, Te, Th, Tl, U, V, W, Zn, and Zr) was developed. 200 µL of either sample material was diluted with an alkaline reagent to a volume of 4 mL in total. Sample dilution and preparation of matrix-matched calibration standards were performed in 48-well plates by an automated liquid handler. Diluted samples were analyzed by inductively coupled plasma mass spectrometry on a Perkin Elmer NexIon 300D ICP-MS instrument equipped with an ESI-FAST SC2DX autosampler in kinetic energy discrimination mode with helium as cell gas at either 4.8 mL or 5.7 mL and 1600 W RF generator power. The method validation results showed good accuracy for fresh human samples from an external quality assessment scheme with measured concentrations within the assigned concentration ranges. Good precision and reproducibility for most elements were demonstrated with variation coefficients below or far below 8% and 15% for whole blood, 8% and 10% for serum, and 10% and 10% for urine, respectively. The developed reagent and instrumental setup were applicable to all three matrices. This minimizes the risk of human errors when switching between analyses of the different sample matrices and allows a rapid and easy analysis of whole blood, serum, and urine within one day if needed. The method demonstrated robustness over time, withstanding minor changes in the preparation of working solutions and samples, instrumental analysis, and setup. Analysis of human real samples showed the method's applicability for 33 toxic and essential elements in whole blood, serum, and urine and at concentrations relevant to clinical diagnostics as well as biomonitoring.

人体基质中有毒和必需元素的分析用于临床诊断和不同人群的生物监测,以研究相关的健康结果。这项工作旨在开发快速可靠的方法,用于分析液态人体基质(如全血、血清和尿液)中的多种元素,并针对三种基质和不同的分析需求采用相似的设置。针对 33 种元素(即 Ag、Al、As、B、Ba、Be、Bi、Cd、Ce、Co、Cr、Cu、Hg、I、Li、Mn、Mo、Ni、Pb、Pd、Pt、Sb、Se、Sn、Sr、Te、Th、Tl、U、V、W、Zn 和 Zr)开发了一种简便快捷的稀释-拍摄法。用碱性试剂将 200 µL 样品材料稀释至总体积为 4 mL。样品稀释和基质匹配校准标准的制备在 48 孔板中通过自动液体处理机进行。稀释后的样品在配备了 ESI-FAST SC2DX 自动进样器的 Perkin Elmer NexIon 300D ICP-MS 仪器上进行电感耦合等离子体质谱分析,采用动能鉴别模式,以 4.8 mL 或 5.7 mL 的氦气作为池气,射频发生器功率为 1600 W。方法验证结果表明,外部质量评估系统对新鲜人体样本的检测具有良好的准确性,测量浓度在指定浓度范围内。大多数元素的精确度和重现性良好,全血的变异系数分别低于或远低于 8%和 15%,血清的变异系数分别低于或远低于 8%和 10%,尿液的变异系数分别低于或远低于 10%和 10%。所开发的试剂和仪器设置适用于所有三种基质。这就最大限度地降低了在不同样品基质之间切换分析时出现人为错误的风险,并能在需要时在一天内快速、简便地分析全血、血清和尿液。随着时间的推移,该方法表现出很强的稳定性,能够经受住工作溶液和样品制备、仪器分析和设置方面的细微变化。对人体真实样本的分析表明,该方法适用于全血、血清和尿液中 33 种有毒和必需元素的分析,其浓度可用于临床诊断和生物监测。
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引用次数: 0
Determination of Ca, P, K, Na, and Mg in Australian Retail Pasteurised Milk Using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP OES). 使用电感耦合等离子体原子发射光谱 (ICP OES) 测定澳大利亚零售巴氏杀菌奶中的钙、磷、钾、钠和镁。
IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL Pub Date : 2024-08-12 eCollection Date: 2024-01-01 DOI: 10.1155/2024/4417607
Duc Doan Nguyen, Vicky Solah, Suzanne Daubney, Saijel Jani

A rapid and simple inductively coupled plasma atomic emission spectrometry (ICP OES) method was developed and validated for the determination of macroelements including calcium (Ca), phosphorus (P), potassium (K), sodium (Na), and magnesium (Mg) in Australian retail pasteurised milk. The milk samples were digested using the mixture of 70% HNO3 and 30% H2O2 (2 : 1, v/v) in an open-tube digester block at 120°C for 4 h. The validated ICP OES method showed good linearity for all elements (R 2 > 0.9993). The method limits of quantification (LOQ) for Ca, P, K, Na, and Mg were 19.85, 8.97, 100.8, 41.92, and 11.56 µg·g-1, respectively. Recoveries were in the range of 91.54-116.0%. Repeatability and interday reproducibility expressed as the relative standard deviation (% RSD) was below 5.0%. The contents of macroelements in 6 retail pasteurised milk samples were between 1099.32 and 1348.65 µg·g-1 (Ca), 914.01 and 1091.21 µg·g-1 (P), 1362.76 and 1549.74 µg·g-1 (K), 288.89 and 323.22 µg·g-1 (Na), and 97.62 and 110.57 µg·g-1 (Mg). Principal component analysis (PCA) revealed that retail pasteurised milk samples were distinctly separated into four groups on the first two principal components (PCs). The difference in the macroelement content between milk brands might be affected by milk regions.

针对澳大利亚零售巴氏杀菌牛奶中钙(Ca)、磷(P)、钾(K)、钠(Na)和镁(Mg)等宏量元素的测定,开发并验证了一种快速简便的电感耦合等离子体原子发射光谱法(ICP OES)。牛奶样品用 70% HNO3 和 30% H2O2(2:1,v/v)的混合物在 120°C 的开放管式消化器中消化 4 小时。Ca、P、K、Na 和 Mg 的方法定量限(LOQ)分别为 19.85、8.97、100.8、41.92 和 11.56 µg-g-1。回收率在 91.54-116.0% 之间。以相对标准偏差(% RSD)表示的重复性和日间重现性低于 5.0%。6 个零售巴氏杀菌奶样品中的宏量元素含量分别为 1099.32 和 1348.65 微克/克-1(钙)、914.01 和 1091.21 微克/克-1(磷)、1362.76 和 1549.74 微克/克-1(钾)、288.89 和 323.22 微克/克-1(钠)以及 97.62 和 110.57 微克/克-1(镁)。主成分分析(PCA)显示,零售巴氏杀菌奶样品在前两个主成分(PCA)上明显分为四组。不同品牌牛奶之间宏量元素含量的差异可能受到牛奶产区的影响。
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Journal of Analytical Methods in Chemistry
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