嗜中性沙门氏菌的特征以及用于区分非典型菌株和典型菌株的 PCR 的开发。

IF 2.2 3区 农林科学 Q2 FISHERIES Journal of fish diseases Pub Date : 2024-11-06 DOI:10.1111/jfd.14028
Teresa Wilson, Mark Green, Vivianne Dunn, David Cummins, Matthew Neave
{"title":"嗜中性沙门氏菌的特征以及用于区分非典型菌株和典型菌株的 PCR 的开发。","authors":"Teresa Wilson, Mark Green, Vivianne Dunn, David Cummins, Matthew Neave","doi":"10.1111/jfd.14028","DOIUrl":null,"url":null,"abstract":"<p><p>This study describes the identification and characterisation of a new mesophilic Aeromonas salmonicida strain, named HMes1 isolated from Atlantic salmon (Salmo salar L.) in Tasmania. Isolates were identified as Aeromonas salmonicida through phenotypic, phylogenetic and genetic characterisation. After characterisation, the diagnostic phenotypic identification system MicroSys A24 was updated and a new multiplex conventional PCR was developed to enable rapid and inexpensive identification of atypical A. salmonicida, and exclusion of the exotic strain, A. salmonicida ssp. salmonicida.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14028"},"PeriodicalIF":2.2000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterisation of a Mesophilic Aeromonas salmonicida and the Development of a PCR to Differentiate Atypical and Typical Strains.\",\"authors\":\"Teresa Wilson, Mark Green, Vivianne Dunn, David Cummins, Matthew Neave\",\"doi\":\"10.1111/jfd.14028\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study describes the identification and characterisation of a new mesophilic Aeromonas salmonicida strain, named HMes1 isolated from Atlantic salmon (Salmo salar L.) in Tasmania. Isolates were identified as Aeromonas salmonicida through phenotypic, phylogenetic and genetic characterisation. After characterisation, the diagnostic phenotypic identification system MicroSys A24 was updated and a new multiplex conventional PCR was developed to enable rapid and inexpensive identification of atypical A. salmonicida, and exclusion of the exotic strain, A. salmonicida ssp. salmonicida.</p>\",\"PeriodicalId\":15849,\"journal\":{\"name\":\"Journal of fish diseases\",\"volume\":\" \",\"pages\":\"e14028\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-11-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of fish diseases\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/jfd.14028\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of fish diseases","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/jfd.14028","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

摘要

本研究描述了从塔斯马尼亚州的大西洋鲑鱼(Salmo salar L.)中分离出的一种新的嗜中性鲑鱼单胞菌(Aeromonas salmonicida)菌株的鉴定和特征描述,该菌株被命名为 HMes1。通过表型、系统发育和遗传特征鉴定,分离物被确定为嗜盐单胞菌。表型鉴定后,对诊断表型鉴定系统 MicroSys A24 进行了更新,并开发了一种新的多重常规 PCR,以快速、低成本地鉴定非典型鲑单胞菌,并排除外来菌株鲑单胞菌(A. salmonicida ssp. salmonicida)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Characterisation of a Mesophilic Aeromonas salmonicida and the Development of a PCR to Differentiate Atypical and Typical Strains.

This study describes the identification and characterisation of a new mesophilic Aeromonas salmonicida strain, named HMes1 isolated from Atlantic salmon (Salmo salar L.) in Tasmania. Isolates were identified as Aeromonas salmonicida through phenotypic, phylogenetic and genetic characterisation. After characterisation, the diagnostic phenotypic identification system MicroSys A24 was updated and a new multiplex conventional PCR was developed to enable rapid and inexpensive identification of atypical A. salmonicida, and exclusion of the exotic strain, A. salmonicida ssp. salmonicida.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of fish diseases
Journal of fish diseases 农林科学-海洋与淡水生物学
CiteScore
4.60
自引率
12.00%
发文量
170
审稿时长
6 months
期刊介绍: Journal of Fish Diseases enjoys an international reputation as the medium for the exchange of information on original research into all aspects of disease in both wild and cultured fish and shellfish. Areas of interest regularly covered by the journal include: -host-pathogen relationships- studies of fish pathogens- pathophysiology- diagnostic methods- therapy- epidemiology- descriptions of new diseases
期刊最新文献
Development of Three Recombinase Polymerase Amplification Assays for the Rapid Visual Detection of Spiroplasma eriocheiris. Does the Infestation by Trematode Parasites Influence Trade-Offs Between Somatic Condition and Male Reproductive Traits in a Viviparous Fish? Inter-Laboratory Comparison of qPCR Assays for Piscirickettsia salmonis in Atlantic Salmon (Salmo salar L.) in 11 Chilean Laboratories. Just Hitching a Ride: Stable Isotopes Reveal Non-Feeding Behaviour of Anisakis simplex Within Its Host Fish. Ray-Resorption Syndrome in European Seabass, Dicentrarchus labrax (Linnaeus, 1758).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1