Carla Vidal - San Martín, José Miguel Bastías-Montes, Yanara Tamarit-Pino, Juan Esteban Reyes-Parra, Mario Pérez-Won
{"title":"脉冲电场(PEF)和低温浓缩(c)对马葵(Aristotelia chilensis (Mol.) Stuntz)和红叶小檗(Berberis microphylla G.)水提取物的理化性质、微生物质量和生物活性化合物的影响","authors":"Carla Vidal - San Martín, José Miguel Bastías-Montes, Yanara Tamarit-Pino, Juan Esteban Reyes-Parra, Mario Pérez-Won","doi":"10.1016/j.fbp.2024.10.021","DOIUrl":null,"url":null,"abstract":"<div><div>The objective of this study was to evaluate the effect of PEF treatment followed by cryoconcentration (c) in aqueous extracts of maqui (<em>Aristotelia chilensis</em> (Mol.) Stuntz) and calafate (<em>Berberis microphylla</em> G.) on their physicochemical properties, microbiological quality, and bioactive compounds. Extracts of maqui and calafate were obtained by aqueous extraction of water-soluble compounds, and then samples were treated by PEF (10 kV/cm, 70 Hz and a pulse width of 10 µs) and pasteurization (90°C/30 s); untreated samples were used as controls. The treated extracts were cryoconcentrated using centrifugation-filtration, and the cryoconcentration parameters included separation efficiency (EFF) and recovered solute (RS). Physicochemical properties were also evaluated, such as soluble solids (SS), titratable acidity (TTA), and viscosity (ƞ), as well as bioactive compound content: total polyphenols (TP), total anthocyanins (TA), total flavonoids (TF) and antioxidant capacity (AOXC). Microbiological quality was evaluated by the counts of the mesophilic aerobic microorganism (MAM) and molds and yeasts (M&Y). The results showed that the EFF of the solutes was higher than 95 % in maqui and 97 % in calafate. As expected, the cryoconcentration process increased TA, ƞ, and SS content. It also significantly increased (p<0.05) in TP, TF, TA, and AOXC content. The microbiological results showed that PEF treatment, together with cryoconcentration and refrigerated storage, maintained the microbiological quality of maqui cryoconcentrates for more than 50 days, whereas in calafate cryoconcentrates, it was maintained for ∼20 days. Moreover, the physicochemical parameters and bioactive compounds of both cryoconcentrates remained stable during the refrigerated storage period. These results demonstrate that the combined use of PEF with cryconcentration seems to be a good option for microbiological and physiochemical stabilization of fruit juices with a high AOXC and for increasing the content of bioactive compounds.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Pulsed Electric Field (PEF) and cryoconcentration (c) in aqueous extracts of maqui (Aristotelia chilensis (Mol.) Stuntz) and calafate (Berberis microphylla G.) on physicochemical properties, microbiological quality, and bioactive compounds\",\"authors\":\"Carla Vidal - San Martín, José Miguel Bastías-Montes, Yanara Tamarit-Pino, Juan Esteban Reyes-Parra, Mario Pérez-Won\",\"doi\":\"10.1016/j.fbp.2024.10.021\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The objective of this study was to evaluate the effect of PEF treatment followed by cryoconcentration (c) in aqueous extracts of maqui (<em>Aristotelia chilensis</em> (Mol.) Stuntz) and calafate (<em>Berberis microphylla</em> G.) on their physicochemical properties, microbiological quality, and bioactive compounds. Extracts of maqui and calafate were obtained by aqueous extraction of water-soluble compounds, and then samples were treated by PEF (10 kV/cm, 70 Hz and a pulse width of 10 µs) and pasteurization (90°C/30 s); untreated samples were used as controls. The treated extracts were cryoconcentrated using centrifugation-filtration, and the cryoconcentration parameters included separation efficiency (EFF) and recovered solute (RS). Physicochemical properties were also evaluated, such as soluble solids (SS), titratable acidity (TTA), and viscosity (ƞ), as well as bioactive compound content: total polyphenols (TP), total anthocyanins (TA), total flavonoids (TF) and antioxidant capacity (AOXC). Microbiological quality was evaluated by the counts of the mesophilic aerobic microorganism (MAM) and molds and yeasts (M&Y). The results showed that the EFF of the solutes was higher than 95 % in maqui and 97 % in calafate. As expected, the cryoconcentration process increased TA, ƞ, and SS content. It also significantly increased (p<0.05) in TP, TF, TA, and AOXC content. The microbiological results showed that PEF treatment, together with cryoconcentration and refrigerated storage, maintained the microbiological quality of maqui cryoconcentrates for more than 50 days, whereas in calafate cryoconcentrates, it was maintained for ∼20 days. Moreover, the physicochemical parameters and bioactive compounds of both cryoconcentrates remained stable during the refrigerated storage period. These results demonstrate that the combined use of PEF with cryconcentration seems to be a good option for microbiological and physiochemical stabilization of fruit juices with a high AOXC and for increasing the content of bioactive compounds.</div></div>\",\"PeriodicalId\":12134,\"journal\":{\"name\":\"Food and Bioproducts Processing\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food and Bioproducts Processing\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0960308524002207\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioproducts Processing","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960308524002207","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Effect of Pulsed Electric Field (PEF) and cryoconcentration (c) in aqueous extracts of maqui (Aristotelia chilensis (Mol.) Stuntz) and calafate (Berberis microphylla G.) on physicochemical properties, microbiological quality, and bioactive compounds
The objective of this study was to evaluate the effect of PEF treatment followed by cryoconcentration (c) in aqueous extracts of maqui (Aristotelia chilensis (Mol.) Stuntz) and calafate (Berberis microphylla G.) on their physicochemical properties, microbiological quality, and bioactive compounds. Extracts of maqui and calafate were obtained by aqueous extraction of water-soluble compounds, and then samples were treated by PEF (10 kV/cm, 70 Hz and a pulse width of 10 µs) and pasteurization (90°C/30 s); untreated samples were used as controls. The treated extracts were cryoconcentrated using centrifugation-filtration, and the cryoconcentration parameters included separation efficiency (EFF) and recovered solute (RS). Physicochemical properties were also evaluated, such as soluble solids (SS), titratable acidity (TTA), and viscosity (ƞ), as well as bioactive compound content: total polyphenols (TP), total anthocyanins (TA), total flavonoids (TF) and antioxidant capacity (AOXC). Microbiological quality was evaluated by the counts of the mesophilic aerobic microorganism (MAM) and molds and yeasts (M&Y). The results showed that the EFF of the solutes was higher than 95 % in maqui and 97 % in calafate. As expected, the cryoconcentration process increased TA, ƞ, and SS content. It also significantly increased (p<0.05) in TP, TF, TA, and AOXC content. The microbiological results showed that PEF treatment, together with cryoconcentration and refrigerated storage, maintained the microbiological quality of maqui cryoconcentrates for more than 50 days, whereas in calafate cryoconcentrates, it was maintained for ∼20 days. Moreover, the physicochemical parameters and bioactive compounds of both cryoconcentrates remained stable during the refrigerated storage period. These results demonstrate that the combined use of PEF with cryconcentration seems to be a good option for microbiological and physiochemical stabilization of fruit juices with a high AOXC and for increasing the content of bioactive compounds.
期刊介绍:
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.