Longxiao Hu , Dandan Li , Qingqing Wei , Li Kang , Yi Sun , Yunliang Jiang
{"title":"鸡卵泡颗粒细胞中新型 IGFBP-2 转录本的特征:mRNA 表达、功能以及生殖激素和 IGF1 的影响","authors":"Longxiao Hu , Dandan Li , Qingqing Wei , Li Kang , Yi Sun , Yunliang Jiang","doi":"10.1016/j.psj.2024.104501","DOIUrl":null,"url":null,"abstract":"<div><div>Insulin-like growth factor binding protein-2 (<strong>IGFBP-2</strong>), a binding protein of insulin-like growth factor (<strong>IGF</strong>) system, regulates the activity of IGFs and also influences cellular function with endogenous activity. In mammals, IGFBP-2 is reported to affect ovarian follicle development and steroidogenesis; however, its role in the chicken ovary is unknown. In this study, we investigated the mRNA expression and function of a novel <em>IGFBP-2</em> transcript and the effect of reproductive hormones and insulin-like growth factor 1 (<strong>IGF1</strong>) on its expression in the ovarian granulosa cells of chicken follicles. The mRNA expression of <em>IGFBP-2</em> was significantly increased in granulosa cells after follicle selection and was higher in hierarchical granulosa cells (<strong>Post-GCs</strong>) than in pre-hierarchical granulosa cells (<strong>Pre-GCs</strong>). IGFBP-2 promoted the proliferation and inhibited the apoptosis of both Pre-GCs and Post-GCs, enhanced the mRNA expression of genes involved in progesterone (<strong>P4</strong>) synthesis in Pre-GCs. However, in Post-GCs, IGFBP-2 inhibited the mRNA expression of these genes and suppressed P4 secretion. The mRNA expression of <em>IGFBP-2</em> was inhibited by estradiol (<strong>E2</strong>) and follicle-stimulating hormone (<strong>FSH</strong>), but enhanced by P4 in Pre-GCs. In Post-GCs, FSH and IGF1 stimulated the mRNA expression of <em>IGFBP-2</em> synergistically. Knockdown of <em>IGFBP-2</em> attenuated the stimulatory effect of IGF1 on the mRNA expression of the side chain cleavage enzyme cytochrome P450 family 11 subfamily A member 1 (<strong><em>CYP11A1</em></strong>). These findings indicate that IGFBP-2 is regulated by FSH and IGF1, exerts different functions in Pre-GCs and Post-GCs in regulating IGF1 and plays an important role in chicken follicle development by affecting granulosa cell proliferation and P4 synthesis.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104501"},"PeriodicalIF":3.8000,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of a novel IGFBP-2 transcript in the ovarian granulosa cells of chicken follicles: mRNA expression, function and effect of reproductive hormones and IGF1\",\"authors\":\"Longxiao Hu , Dandan Li , Qingqing Wei , Li Kang , Yi Sun , Yunliang Jiang\",\"doi\":\"10.1016/j.psj.2024.104501\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Insulin-like growth factor binding protein-2 (<strong>IGFBP-2</strong>), a binding protein of insulin-like growth factor (<strong>IGF</strong>) system, regulates the activity of IGFs and also influences cellular function with endogenous activity. In mammals, IGFBP-2 is reported to affect ovarian follicle development and steroidogenesis; however, its role in the chicken ovary is unknown. In this study, we investigated the mRNA expression and function of a novel <em>IGFBP-2</em> transcript and the effect of reproductive hormones and insulin-like growth factor 1 (<strong>IGF1</strong>) on its expression in the ovarian granulosa cells of chicken follicles. The mRNA expression of <em>IGFBP-2</em> was significantly increased in granulosa cells after follicle selection and was higher in hierarchical granulosa cells (<strong>Post-GCs</strong>) than in pre-hierarchical granulosa cells (<strong>Pre-GCs</strong>). IGFBP-2 promoted the proliferation and inhibited the apoptosis of both Pre-GCs and Post-GCs, enhanced the mRNA expression of genes involved in progesterone (<strong>P4</strong>) synthesis in Pre-GCs. However, in Post-GCs, IGFBP-2 inhibited the mRNA expression of these genes and suppressed P4 secretion. The mRNA expression of <em>IGFBP-2</em> was inhibited by estradiol (<strong>E2</strong>) and follicle-stimulating hormone (<strong>FSH</strong>), but enhanced by P4 in Pre-GCs. In Post-GCs, FSH and IGF1 stimulated the mRNA expression of <em>IGFBP-2</em> synergistically. Knockdown of <em>IGFBP-2</em> attenuated the stimulatory effect of IGF1 on the mRNA expression of the side chain cleavage enzyme cytochrome P450 family 11 subfamily A member 1 (<strong><em>CYP11A1</em></strong>). These findings indicate that IGFBP-2 is regulated by FSH and IGF1, exerts different functions in Pre-GCs and Post-GCs in regulating IGF1 and plays an important role in chicken follicle development by affecting granulosa cell proliferation and P4 synthesis.</div></div>\",\"PeriodicalId\":20459,\"journal\":{\"name\":\"Poultry Science\",\"volume\":\"103 12\",\"pages\":\"Article 104501\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0032579124010794\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0032579124010794","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Characterization of a novel IGFBP-2 transcript in the ovarian granulosa cells of chicken follicles: mRNA expression, function and effect of reproductive hormones and IGF1
Insulin-like growth factor binding protein-2 (IGFBP-2), a binding protein of insulin-like growth factor (IGF) system, regulates the activity of IGFs and also influences cellular function with endogenous activity. In mammals, IGFBP-2 is reported to affect ovarian follicle development and steroidogenesis; however, its role in the chicken ovary is unknown. In this study, we investigated the mRNA expression and function of a novel IGFBP-2 transcript and the effect of reproductive hormones and insulin-like growth factor 1 (IGF1) on its expression in the ovarian granulosa cells of chicken follicles. The mRNA expression of IGFBP-2 was significantly increased in granulosa cells after follicle selection and was higher in hierarchical granulosa cells (Post-GCs) than in pre-hierarchical granulosa cells (Pre-GCs). IGFBP-2 promoted the proliferation and inhibited the apoptosis of both Pre-GCs and Post-GCs, enhanced the mRNA expression of genes involved in progesterone (P4) synthesis in Pre-GCs. However, in Post-GCs, IGFBP-2 inhibited the mRNA expression of these genes and suppressed P4 secretion. The mRNA expression of IGFBP-2 was inhibited by estradiol (E2) and follicle-stimulating hormone (FSH), but enhanced by P4 in Pre-GCs. In Post-GCs, FSH and IGF1 stimulated the mRNA expression of IGFBP-2 synergistically. Knockdown of IGFBP-2 attenuated the stimulatory effect of IGF1 on the mRNA expression of the side chain cleavage enzyme cytochrome P450 family 11 subfamily A member 1 (CYP11A1). These findings indicate that IGFBP-2 is regulated by FSH and IGF1, exerts different functions in Pre-GCs and Post-GCs in regulating IGF1 and plays an important role in chicken follicle development by affecting granulosa cell proliferation and P4 synthesis.
期刊介绍:
First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers.
An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.