鸡卵泡颗粒细胞中新型 IGFBP-2 转录本的特征:mRNA 表达、功能以及生殖激素和 IGF1 的影响

IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Poultry Science Pub Date : 2024-11-02 DOI:10.1016/j.psj.2024.104501
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引用次数: 0

摘要

胰岛素样生长因子结合蛋白-2(IGFBP-2)是胰岛素样生长因子(IGF)系统的一种结合蛋白,它调节 IGFs 的活性,并以内源性活性影响细胞功能。据报道,IGFBP-2 在哺乳动物中影响卵泡发育和类固醇生成,但在鸡卵巢中的作用尚不清楚。本研究调查了一种新型 IGFBP-2 转录本的 mRNA 表达和功能,以及生殖激素和胰岛素样生长因子 1(IGF1)对其在鸡卵泡颗粒细胞中表达的影响。卵泡选择后颗粒细胞中IGFBP-2的mRNA表达量明显增加,分层颗粒细胞(Post-GCs)中IGFBP-2的mRNA表达量高于分层前颗粒细胞(Pre-GCs)。IGFBP-2能促进Pre-GCs和Post-GCs的增殖并抑制其凋亡,增强Pre-GCs中参与孕酮(P4)合成的基因的mRNA表达。然而,在后前叶细胞中,IGFBP-2 会抑制这些基因的 mRNA 表达并抑制 P4 的分泌。雌二醇(E2)和促卵泡激素(FSH)会抑制 IGFBP-2 的 mRNA 表达,但 P4 则会增强前 GCs 中 IGFBP-2 的 mRNA 表达。在Post-GCs中,FSH和IGF1协同刺激IGFBP-2的mRNA表达。敲除 IGFBP-2 可减轻 IGF1 对侧链裂解酶细胞色素 P450 家族 11 亚家族 A 成员 1(CYP11A1)mRNA 表达的刺激作用。这些研究结果表明,IGFBP-2受FSH和IGF1调控,在前GCs和后GCs中发挥不同的调节IGF1的功能,并通过影响颗粒细胞增殖和P4合成在鸡卵泡发育中发挥重要作用。
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Characterization of a novel IGFBP-2 transcript in the ovarian granulosa cells of chicken follicles: mRNA expression, function and effect of reproductive hormones and IGF1
Insulin-like growth factor binding protein-2 (IGFBP-2), a binding protein of insulin-like growth factor (IGF) system, regulates the activity of IGFs and also influences cellular function with endogenous activity. In mammals, IGFBP-2 is reported to affect ovarian follicle development and steroidogenesis; however, its role in the chicken ovary is unknown. In this study, we investigated the mRNA expression and function of a novel IGFBP-2 transcript and the effect of reproductive hormones and insulin-like growth factor 1 (IGF1) on its expression in the ovarian granulosa cells of chicken follicles. The mRNA expression of IGFBP-2 was significantly increased in granulosa cells after follicle selection and was higher in hierarchical granulosa cells (Post-GCs) than in pre-hierarchical granulosa cells (Pre-GCs). IGFBP-2 promoted the proliferation and inhibited the apoptosis of both Pre-GCs and Post-GCs, enhanced the mRNA expression of genes involved in progesterone (P4) synthesis in Pre-GCs. However, in Post-GCs, IGFBP-2 inhibited the mRNA expression of these genes and suppressed P4 secretion. The mRNA expression of IGFBP-2 was inhibited by estradiol (E2) and follicle-stimulating hormone (FSH), but enhanced by P4 in Pre-GCs. In Post-GCs, FSH and IGF1 stimulated the mRNA expression of IGFBP-2 synergistically. Knockdown of IGFBP-2 attenuated the stimulatory effect of IGF1 on the mRNA expression of the side chain cleavage enzyme cytochrome P450 family 11 subfamily A member 1 (CYP11A1). These findings indicate that IGFBP-2 is regulated by FSH and IGF1, exerts different functions in Pre-GCs and Post-GCs in regulating IGF1 and plays an important role in chicken follicle development by affecting granulosa cell proliferation and P4 synthesis.
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来源期刊
Poultry Science
Poultry Science 农林科学-奶制品与动物科学
CiteScore
7.60
自引率
15.90%
发文量
0
审稿时长
94 days
期刊介绍: First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
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