利用腊菊水提取物绿色合成的氧化锌纳米颗粒的抗糖尿病、抗炎、抗氧化和细胞毒性潜力。

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI:10.1007/s13205-024-04125-0
Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju
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引用次数: 0

摘要

目前的研究涉及利用腊菊嫩枝的水提取物合成氧化锌纳米颗粒(ZnO-NPs),并随后通过不同的分析方法进行表征,如紫外可见光谱、扫描电子显微镜(SEM)、能量色散 X 射线光谱(EDX)、X 射线衍射(XRD)、透射电子显微镜(TEM)和 zeta 电位。然后,通过一系列分析,包括细胞毒性、抗氧化、抗炎和酶抑制抗糖尿病作用,测试了 ZnO-NPs 对 C3A 肝细胞和 L6 肌细胞的生物效应。紫外可见光谱分析显示,该化合物在 360 波长处有最大吸收光谱;TEM 分析显示,该化合物呈球形和六边形结构,平均尺寸为 28.05-58.3 nm;XRD 显示,该化合物呈结晶六边形结构。zeta电位评估表明,ZnO-NPs在- 20 mV下相对稳定,傅立叶变换红外光谱分析确定了一些与酚类、羧酸和酰胺相关的重要官能团,它们是还原和稳定ZnO-NPs的主要成分。合成的 ZnO-NPs 在较高浓度(125 微克/毫升和 250 微克/毫升)时对细胞系有细胞毒性作用,这使得炎症和抗氧化检测结果的解释变得复杂。然而,在较高浓度下(125 微克/毫升和 250 微克/毫升),锰氧化物对细胞株的细胞毒性作用明显(p
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Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of Helichrysum cymosum.

The current research involved the synthesis of zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of Helichrysum cymosum shoots, and subsequent characterization via different analytical methods, such as UV-Vis spectroscopy, Scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Transmission electron microscope (TEM), and zeta potential. The biological effects of the ZnO-NPs were then tested against C3A hepatocyte cells and L6 myocyte cell lines via series of analysis, including cytotoxicity, antioxidant, anti-inflammatory, and antidiabetic effect via enzymatic inhibition. The UV-Vis analysis showed a maximum absorption spectrum at 360, and the TEM analysis reveals a spherical and hexagonal structures, with an average dimension of 28.05-58.3 nm, and the XRD reveals a crystalline hexagonal structure. The zeta potential evaluation indicated that the ZnO-NPs are relatively stable at - 20 mV, and the FTIR analysis identified some important functional group associated with phenolics, carboxylic acid, and amides that are responsible for reducing and stabilizing the ZnO-NPs. The synthesized ZnO-NPs demonstrated cytotoxic effects on the cell lines at higher concentrations (125 µg/mL and 250 µg/mL), complicating the interpretation of the results of the inflammatory and antioxidant assays. However, there was a significant (p < 0.05) increase in the inhibitions of pancreatic lipase, alpha-glucosidase, and alpha-amylase, indicating beneficial antidiabetic effects.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
期刊最新文献
Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of Helichrysum cymosum. Bioinformatics analysis and experimental validation of the oncogenic role of COL11A1 in pan-cancer. Nanomaterial-enabled drug transport systems: a comprehensive exploration of current developments and future avenues in therapeutic delivery. Comparative genomics of zoonotic pathogen Clostridioides difficile of animal origin to understand its diversity. Discovery and characterization of novel lipopeptides produced by Virgibacillus massiliensis with biosurfactant and antimicrobial activities.
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