用于皮肤美容的海薰衣草(Limonium algarvense Erben)种子提取物的抗氧化活性、酶抑制作用、光保护作用、细胞毒性和植物化学成分分析

IF 2.5 Q2 MULTIDISCIPLINARY SCIENCES Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2024-11-08 DOI:10.1186/s43088-024-00576-9
Leonardo Lescano, Zoltán Cziáky, İnci Kurt-Celep, Gökhan Zengin, Eliana Fernandes, Riccardo Trentin, Catarina G. Pereira, Luísa Custódio, Maria João Rodrigues
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引用次数: 0

摘要

背景尽管海熏衣草是一种药用植物,但对其种子的生物特性和化学成分的研究尚未开展。因此,本研究评估了不同萃取溶剂对温室栽培的海薰衣草种子的生物活性和化学成分的影响,旨在研究其作为皮肤美容成分的潜在用途。因此,研究人员检测了乙酸乙酯、丙酮、乙醇和水提取物的抗氧化活性、酶抑制作用、光保护作用和细胞毒性,然后通过分光光度法进行植物化学分析,并通过超高效液相色谱-电喷雾离子化质谱法(UHPLC-Esi-MS/MS)进行进一步详细分析。结果水提取物在清除 1,1-二苯基-2-苦基肼(DPPH)和 2,2′-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基、还原铁和螯合铜(分别为 296、478、230 和 678 µg/mL)方面的半数最大有效浓度(EC50)值较低,证明其具有显著的抗氧化活性。乙醇提取物在抑制化妆品相关酶方面更为有效,尤其是弹性蛋白酶和透明质酸酶(分别为 2.18 微克/毫升和 3.21 微克/毫升)。水和丙酮提取物的防晒系数最高(分别为 23.2 和 18.9)。所有萃取物对哺乳动物细胞系都没有或只有微弱的细胞毒性(细胞存活率为 70-120%)。水提取物中的酚类和缩合单宁含量最高(分别为 115 毫克/克和 78.30 毫克/克),而乙醇提取物中的黄酮类含量最高(62.73 毫克/克)。UHPLC-ESI-MS/MS分析确定没食子酸乙酯、杨梅素、芦丁和槲皮素是乙醇提取物的主要成分,而杨梅素-O-芸香苷异构体在水提取物中占主导地位。
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Antioxidant activity, enzyme inhibition, photoprotection, cytotoxicity, and phytochemical profiling of sea lavender (Limonium algarvense Erben) seed extracts for dermo-cosmetic use

Background

Despite sea lavender being a medicinal species, research on its seeds’ biological properties and chemical composition is unexplored. Thus, this study evaluated the effect of different extraction solvents on the biological activities and chemical profile of greenhouse-cultivated sea lavender seeds, aiming at their potential use as a dermo-cosmetic ingredient. Therefore, ethyl acetate, acetone, ethanol, and water extracts were examined for their antioxidant activity, enzyme inhibition, photoprotection, and cytotoxicity, followed by phytochemical analysis through spectrophotometric methods, further detailed by Ultrahigh-Performance Liquid Chromatography Coupled with Electrospray Ionization Mass/Mass Spectrometry (UHPLC-Esi-MS/MS).

Results

The water extract demonstrated significant antioxidant activity, evidenced by low half maximal effective concentration (EC50) values in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing iron and chelating copper (296, 478, 230 and 678 µg/mL, respectively). The ethanol extract was more effective in inhibiting cosmetic-related enzymes, particularly elastase and hyaluronidase (2.18 and 3.21 µg/mL, respectively). The water and acetone extracts had the highest sun protection factors (23.2 and 18.9, respectively). All the extracts had nil to weak cytotoxicity (70–120% cell viability) towards mammalian cell lines. The water extract had the highest phenolics and condensed tannins (115 and 78.30 mg/g extract, respectively), while the ethanol contained the most flavonoids (62.73 mg/g extract). UHPLC-ESI–MS/MS analysis identified ethyl gallate, myricetin, rutin, and quercetin as major components of the ethanol extract, whereas myricetin-O-rutinoside isomers are predominant in the water extract.

Conclusions

These findings highlight the potential of greenhouse-cultivated sea lavender seeds as potential dermo-cosmetic ingredients, with ethanol and water extracts demonstrating superior biological activities and chemical profiles, significantly contributing to general skin health and protection.

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期刊介绍: Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.
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