Kang Wei, Jin-Li Ding, Hang-Rong Xu, Ming-Guang Feng, Sheng-Hua Ying
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引用次数: 0
摘要
昆虫病原真菌会排泄一组蛋白质，以吸收营养并击败宿主的免疫防御。在重组菌株中有效分泌毒力相关蛋白需要功能性分泌信号序列。本研究利用分泌组分析来探索 Beauveria bassiana 的分泌蛋白。富集分析表明，B. bassiana分泌组主要与葡萄糖苷、多糖、胞外酯类化合物等的代谢有关。此外，还富集了与细胞成分的生物生成有关的蛋白质，包括参与细胞壁和液泡生物生成的蛋白质。然后，以绿色荧光蛋白为报告基因，对四个分泌信号序列进行了功能验证。最后，利用一个信号序列在 B. bassiana 中排泄三种昆虫毒液蛋白血清素，其中血清素 8 基因的过度表达导致真菌毒力显著增强。这项研究强调，功能性分泌信号序列是昆虫致病真菌排泄毒力相关蛋白的潜在分子元素。
Exploring secretory signal sequences useful in excreting recombinant proteins in Beauveria bassiana as biocontrol fungus
Entomopathogenic fungi excrete a group of proteins to assimilate nutrients and defeat the host immune defense. Functional secretory signal sequences are needed for efficient secretion of the virulence-related proteins in recombinant strain. In this study, secretome analysis was used to explore the secreted proteins of Beauveria bassiana. Enrichment analysis indicated that B. bassiana secretome was mainly associated with metabolism of glucoside, polysaccharide, extracellular ester compound, and so on. In addition, proteins associated with biogenesis of cellular components were also enriched, including those involved in biogenesis of cell wall and vacuole. Then, four secretory signal sequences were functionally verified with green fluorescent protein as reporter. Finally, a signal sequence was used to excrete three insect venom protein serpins in B. bassiana, in which over-expression of serpin 8 gene resulted in a significant increase in fungal virulence. This study highlights that functional secretory signal sequences are potential molecular elements useful in excretion of virulence-related proteins in insect pathogenic fungi.
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