Timur Sh. Atabaev, Dinara Askar, Zarina Baranchiyeva, Balnur A. Zhainsabayeva, Timur Elebessov, Moon Sung Kang, Bakyt Duisenbayeva, Ellina A. Mun, Tri Thanh Pham and Dong-Wook Han
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引用次数: 0
摘要
以碳点为基础的放射对比剂因其较好的对比能力、简单的合成方案、较高的胶体稳定性和良好的生物相容性,最近引发了研究人员的兴趣。在本研究中,我们首次提出使用柠檬酸(C6H8O7)、尿素(CH4N2O)和碘化钾(KI)等低成本试剂合成碘掺杂碳点(I-CDs)。所制备的 I-CD 具有优异的胶体稳定性(zeta 电位值为 -64.7 mV)、激发相关荧光特性(最大量子产率为 8.9%)和平均碘浓度为 4.67 wt%。值得注意的是,与市场上使用的碘普罗米德放射性对比剂(30.98 HU mL mg-1)相比,制备的 I-CD 具有更高的 X 射线衰减效率(42.87 HU mL mg-1)。此外,ATPase 活性、HeLa、NHDF、HEK293 和 A549 细胞系的细胞毒性分析以及果蝇神经细胞在完整脑叶中的活细胞成像实验表明,制备的 I-CD 具有很高的生物相容性和无毒性。总之,生物相容性好且成本低廉的 I-CDs 作为双功能放射对比剂和荧光剂在生物医学应用中大有可为。
Biocompatible and low-cost iodine-doped carbon dots as a bifunctional fluorescent and radiocontrast agent for X-ray CT imaging†
Carbon dot-based radiocontrast agents have recently sparked the interest of researchers owing to their better contrasting capabilities, simple synthesis protocols, high colloidal stability, and good biocompatibility. In this study, we propose for the first time the synthesis of iodine-doped carbon dots (I-CDs) using low-cost reagents such as citric acid (C6H8O7), urea (CH4N2O) and potassium iodide (KI). The as-prepared I-CDs demonstrated excellent colloidal stability (with a zeta potential value of −64.7 mV), excitation-dependent fluorescent properties (with a maximum quantum yield of ∼8.9%), and a mean iodine concentration of ∼4.67 wt%. Notably, the as-prepared I-CDs displayed greater X-ray attenuation efficiency (42.87 HU mL mg−1) as compared to the commercially employed iopromide radiocontrast agent (30.98 HU mL mg−1). Furthermore, ATPase activity, cytotoxicity analysis with HeLa, NHDF, HEK293, and A549 cell lines, and live-cell imaging experiments of the Drosophila neuroblasts in intact brain lobes suggested high biocompatibility and nontoxicity of the prepared I-CDs. Overall, biocompatible and low-cost I-CDs show great promise as bifunctional radiocontrast and fluorescent agents for biomedical applications.